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Environmental Chemistry Environmental Chemistry Society
Environmental problems - Chemical approaches
RESEARCH ARTICLE

Reactivity of antimony(V) and its effect on the pro-inflammatory response in the RAW 264.7 monocyte/macrophage cell line

Nicole Canto A , Luis Mercado A and Waldo Quiroz https://orcid.org/0000-0003-4727-1089 A B
+ Author Affiliations
- Author Affiliations

A Pontificia Universidad Católica de Valparaíso. Facultad de Ciencias, Instituto de Química, Instituto de Biología, Avenida Universidad 330, Curauma, Valparaíso 3100000, Chile.

B Corresponding author. Email: waldo.quiroz@pucv.cl

Environmental Chemistry 17(2) 173-181 https://doi.org/10.1071/EN19173
Submitted: 8 June 2019  Accepted: 9 September 2019   Published: 30 October 2019

Environmental context. Antimony is found at high concentrations in airborne particulate matter in urban cities. Contaminants that enter humans through the breathing of fine particulate matter are released directly into the blood after overcoming the first barrier of the immune system including macrophages. We investigated the effects of antimony on the inflammatory response in macrophages and its potentially harmful effects.

Abstract. Antimony (Sb) is a metalloid whose increased presence in atmospheric particulate matter is of concern since its effects on living beings are unknown. The present study aims to characterise the redox chemical changes of SbV in vitro using a murine macrophage cell line, RAW 264.7. Analysis of the redox chemical changes revealed that SbV is partially reduced to SbIII when in culture. SbV induces the formation of reactive oxygen species, which may be causally related to the reduction of this chemical species. The expression of pro-inflammatory genes, which includes TNF-α and COX-2, was upregulated in response to SbV. The secretion of TNF-α protein was significantly higher from cells treated with SbV than the untreated controls. The pro-inflammatory response to SbV was determined to be dose-dependent. The signalling pathway underlying the cellular response to SbV is likely to be independent from the TLR4-mediated mechanism of lipopolysaccharide activation. This is the first study to describe the potentially pro-inflammatory effects of SbV on mammalian cells.

Additional keywords: antimony, cytokines, metalloid, speciation, toxicity.


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