An isotope injection technique to measure agronomic effectiveness of different phosphate sources in undisturbed soils

Abstract

This paper describes the use of an isotope injection technique for assessing the agronomic effectiveness of phosphate sources in undisturbed soils in field and glasshouse experiments. A special injection apparatus consisting of 20 syringe needles linked to a common reservoir was used to label soil cores 150 mm in diameter and 150 mm in height, without significantly disturbing the soil structure and established perennial ryegrass (Lolium perenne) root patterns. Variations in the amount of phosphorus (P) taken up by plants from single superphosphate (applied at 30, 60, and 100 kg P/ha), from 30% acidulated North Carolina phosphate rock (60 g P/ha), and from 'as received' North Carolina phosphate rock (60 kg P/ha) were indicated by the specific activity of the plant P. Percentages of plant P derived from the fertilisers were calculated on the basis of the specific activity of the fertilised treatments and the controls. The technique provides a simple method of comparing the agronomic effectiveness of phosphate sources in undisturbed soils and, unlike total dry matter yield and P uptake, is relatively unaffected by environmental factors.