Characterisation of naturalised strains of Trifolium subterraneum var. brachycalycinum cv. Clare in northern New South Wales

Abstract

Burrs were collected from paddocks on 3 properties in northern New South Wales where the age of the Trifolium subterraneum var. brachycalycinum cv. Clare swards varied from 19 to 28 years. At 1 site burrs were also sampled from swards sown 2 and 10 years previously. Twenty seedlings from these burrs and 20 plants of certified cv. Clare were grown as spaced plants in a nursery. These were assessed for vegetative and floral characters, flowering time, number of seeds per burr, seed weight, and percentage hardseed after storage at 25/25¦C for 6 months and 25/45¦C for a further 6 months. For most plants the mean number of days from sowing to first flower was similar to that of Clare. Compared with the naturalised strains, Clare had the lowest (P<0.05) mean number of seeds per burr: about 25% below the mean of the strains (2.7 seeds per burr). While the lowest mean seed weights of the strains were not significantly different from those of Clare, the seed weights of plants from 3 sites were higher (P<0.05) than those of Clare. After storage for either 6 or 12 months, hardseed levels were also lowest (P<0.05) for Clare. Plants from the 2-year-old sward had the same median number of seeds per burr (2.0) as Clare. As sward age increased, the median number of seeds per burr increased to 2.8. Hardseed percentages were lowest for plants of Clare and for those from the 2-year-old sward after 6 months, and for Clare after 12 months. These studies indicated the presence of divergent strains in old swards of Clare in a summer rainfall environment. Natural selection among variability within Clare is the most likely reason for the development of these strains in an environment marginal for the long-term persistence of this softseeded cultivar. Although strains had the same vegetative and floral markings as Clare, differences in ecologically important characters such as number of seeds per burr, seed weight, and hardseededness may result in plants that are better adapted to the environment in which they evolved. From these studies 23 plants of T. subterraneum var. brachycalycinum were selected for further evaluation.