Gene expression profiling of Hereford Shorthorn cattle following challenge with Boophilus microplus tick larvae
Y. H. Wang A C , A. Reverter A , D. Kemp A , S. M. McWilliam A , A. Ingham A , C. A. Davis A , R. J. Moore B and S. A. Lehnert AA CSIRO Livestock Industries, Queensland Bioscience Precinct, 306 Carmody Road, St Lucia, Brisbane, Qld 4067, Australia.
B CSIRO Livestock Industries, Australian Animal Health Laboratory, Private Bag 24, Geelong, Vic. 3220, Australia.
C Corresponding author. Email: yonghong.wang@csiro.au
Australian Journal of Experimental Agriculture 47(12) 1397-1407 https://doi.org/10.1071/EA07012
Submitted: 15 January 2007 Accepted: 9 May 2007 Published: 16 November 2007
Abstract
The ability of cattle to resist tick infestations is partly genetically determined. In order to better define the nature of Bos taurus resistance to the cattle tick Boophilus microplus, skin gene expression was studied using a cattle skin derived cDNA microarray. Expression profiles were determined in skin biopsies sampled from three highly tick resistant animals (HR) and two animals with lower tick resistance (LR) at time 0, immediately before challenge, and again 24 h after challenge. The analysis of the resulting expression data addressed two biological questions: first, for any animal exposed to ticks, which genes are differentially expressed in the 24 h following challenge; and second, which genes are differentially expressed between animals of high and low resistance at 24 h after challenge? In total, 214 genes were found to be differentially expressed in response to larval challenge across all the animals. Seventy-two genes were upregulated and 76 were downregulated at 24 h after challenge. Genes with significantly altered gene expression levels following tick infestation were predominantly keratin genes or mitochondrial genes, as well as odorant binding protein (OBP) and Bos taurus major allergen BDA20. In addition, we identified 66 genes with differential expression between HR and LR animals at 24 h. Of these, genes representing the extracellular matrix and immunoglobulin gene expression pathways were overrepresented. Three differentially expressed genes, OBP, Bos taurus major allergen BDA20 and dendritic cell protein HFL-B5 were further analysed by quantitative reverse transcription PCR (qRT-PCR). The qRT-PCR assay results closely mirrored the expression profiles found in the microarray experiment.
Acknowledgements
After this manuscript was submitted to AJEA, our coauthor, Dr David Kemp lost his battle against illness. The authors would like to dedicate this manuscript to commemorate David’s great contribution during the experimental design, sample collection, manuscript preparation and discussion. The authors would also like to acknowledge Peter Willadsen, Bill Barendse and Ross Tellam for helpful scientific discussion, Nick Corbet, Graeme Halford, John Quilty, Warren Sim and Paul Williams for tick counts and cattle management and Rex Holmes, George Riding and Gustavo Sabatini for technical support in skin sampling.
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