Molecular and bioinformatic strategies for gene discovery for meat traits: a reverse genetics approach
I. J. Hagen A , A. Zadissa A , J. C. McEwan B , B. A. Veenvliet B , S. M. Hickey C , N. G. Cullen C , C. A. Morris C and T. Wilson A DA AgResearch Molecular Biology Unit, Department of Biochemistry, University of Otago, Dunedin, New Zealand.
B AgResearch Invermay Research Centre, Mosgiel, New Zealand.
C AgResearch Ruakura Research Centre, Hamilton, New Zealand.
D Corresponding author. Email: theresa.wilson@agresearch.co.nz
Australian Journal of Experimental Agriculture 45(8) 801-807 https://doi.org/10.1071/EA05044
Submitted: 15 February 2005 Accepted: 23 May 2005 Published: 26 August 2005
Abstract
The identification of genes that influence meat quality and meat yield has relevance for several livestock species. Candidate genes include those involved in the biochemical pathways controlling muscle differentiation, growth and development. Mutations in one such gene, myostatin, have previously been reported to have dramatic effects on muscle phenotype in cattle. Here we report a screening strategy for the discovery of novel mutations in 10 genes involved in muscle development using single-stand conformation polymorphism gels and DNA sequencing. Several novel mutations, both non-synonymous and synonymous were discovered, and some of these may alter gene function. In addition, we also conducted a meta-analysis of published quantitative trait loci from cattle, sheep, pigs and mice, identifying those muscle development genes most likely to contribute to variation in muscle traits within species. From this strategy we found several genes that map into regions that are part of the extended muscle development pathway.
Additional keywords: meat yield, mutation screening, myostatin.
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