Free Standard AU & NZ Shipping For All Book Orders Over $80!
Register      Login
Animal Production Science Animal Production Science Society
Food, fibre and pharmaceuticals from animals
RESEARCH ARTICLE

Effect of timing of protein supplementation on the performance of lactating dairy cows

B. C. Granzin
+ Author Affiliations
- Author Affiliations

NSW Department of Primary Industries, Wollongbar Agricultural Institute, Wollongbar, NSW 2477, Australia. Email: brad.granzin@dpi.nsw.gov.au

Australian Journal of Experimental Agriculture 45(4) 337-345 https://doi.org/10.1071/EA03209
Submitted: 28 October 2003  Accepted: 4 June 2004   Published: 23 May 2005

Abstract

Two experiments were undertaken to determine the effect of timing of protein supplementation on performance of grazing, lactating Holstein–Friesian cows fed maize silage and grain-based concentrate. In experiment 1, 36 cows were fed 0.8 kg DM/day of solvent-extracted cottonseed meal (CSM) either as 1 meal at 1200 hours with maize silage (CSM 1200) or at 1530 hours with concentrate (CSM 1530), or in 2 meals at 0600 and 1530 hours with concentrate (CSM 600 + 1530). In experiment 2, 36 cows were either fed no CSM (control) or fed 1.0 kg DM/day as either CSM 1200 or CSM 600 + 1530.

In experiment 1, daily yields of 4% fat-corrected milk (FCM) and milk fat for CSM 600 + 1530 were significantly higher than for CSM 1530 with respective means of 22.8 v. 20.7 L and 895 v. 804 g. Daily yields of FCM and milk fat for CSM 1200 were intermediate (21.7 L and 841 g/cow, respectively). A similar trend for daily protein yield per cow was noted (712, 695 and 666 g for CSM 600 + 1530, 1200 and 1530, respectively). In experiment 2, milk yield differed numerically between CSM 600 + 1530 and other treatments, with means (± s.e.d.) of 24.7 ± 0.78, 22.9 ± 0.78 and 22.9 ± 0.78 L/cow.day for CSM 600 + 1530, CSM 1200 and control, respectively. Mean (± s.e.d.) net energy requirements for milk production and liveweight change tended to be lower for the control (68 ± 3.6 MJ/cow.day) as opposed to CSM 600 + 1530 (79 ± 3.6 MJ/cow.day) and CSM 1200 (76 ± 3.6 MJ/cow.day).

Cumulative time where rumen degradable nitrogen:rumen degradable dry matter was less than 22 g/kg were 2, 2 and 3 h for CSM 600 + 1530, CSM 1200 and CSM 1530, respectively, in experiment 1, and 6, 4 and 2 h for the control, 1200 and CSM 600 + 1530, respectively, in experiment 2. No differences in rumen ammonia-N concentrations were noted between treatments in experiment 1. In experiment 2, a significantly lower mean (± s.e.d.) rumen ammonia-N concentration was recorded for the control at 1530 hours (62 ± 14.1 mg/dL) in comparison to CSM 600 + 1530 (114 ± 14.1 mg/dL) and CSM 1200 (119 ± 14.1 mg/dL).

These experiments show that for grazing dairy cows supplemented with maize silage and grain-based concentrate, feeding a daily aliquot of CSM as 2 meals at 0600 and 1530 hours rather than 1 meal at 1200 or 1530 hours improves milk production.


Acknowledgments

The author thanks Dick Bryant, Bill Davis, Scott Petty and Neil McAnelly for their technical assistance throughout this experiment. The editorial comments of Professor Tom Cowan, Professor Bill Fulkerson, Dr Alan Kaiser and Mr Robin Dobos are acknowledged.


References


AOAC (1970) Qualitative tests for protein. Dumas method (7.017). In ‘Official methods of analysis’. 11th edn. p. 123. (Association of Official Analytical Chemists: Washington DC)

Chen XB (1995) ‘NEWAY Excel: an excel application program for processing feed degradability data.’ (International Feed Resources Unit, Rowett Research Institute: Bucksburn, Aberdeen)

Cowan RT, Moss RJ, Goodwin PG, Fulkerson WJ (1998) Efficiency in feeding systems for subtropical dairy production. Proceedings of the Australian Society of Animal Production 22, 125–128. open url image1

CPM Dairy (1998) ‘CPM Dairy version 1.0: a system for evaluating and formulating dairy cow rations’. (Center for Animal Health and Productivity, School of Veterinary Medicine, University of Pennsylvania: Kennett Square, PA; The Department of Animal Science, Cornell University: Ithaca, NY and; the William H. Miner Agricultural Research Institute: Chazy, NY)

Czerkawski JW (1986) ‘An introduction to rumen studies.’ (Permagmon Press: Oxford)

Dewhurst RJ, Davies DR, Merry RJ (2000) Microbial protein supply from the rumen. Animal Feed Science and Technology 85, 1–21.
Crossref | GoogleScholarGoogle Scholar | open url image1

Earle DF, McGowan AA (1979) Evaluation and calibration of an automated rising plate meter for estimating dry matter yield of pasture. Australian Journal of Experimental Agriculture and Animal Husbandry 19, 337–343.
Crossref |
open url image1

Fulkerson WJ, Slack K (1993) Estimating mass of temperate and tropical pastures in the subtropics. Australian Journal of Experimental Agriculture 33, 865–869.
Crossref |
open url image1

Fulkerson WJ, Fennell J, Slack K (2000) Production and forage quality of prairie grass (Bromus willdenowii) in comparison to perennial ryegrass (Lolium perenne) and tall fescue (Festuca arundinacea) in subtropical dairy pastures. Australian Journal of Experimental Agriculture 40, 1059–1067.
Crossref | GoogleScholarGoogle Scholar | open url image1

Gaines WL, Davidson FA (1923) Relationship between percent fat content and yield of milk. Illinios Agricultural Experimental Station Bulletin No. 245.

Granzin BC (2000) Rumen degradation rates of protein and carbohydrate fractions of kikuyu and ryegrass grown in a subtropical region. Asian–Australasian Journal of Animal Sciences 13, 217. open url image1

Granzin BC (2004) The rumen degradability of grains subjected to various forms of processing. Proceedings of the Australian Dairy Science Symposium 1, 41. open url image1

Kaiser AG, Havilah EJ, Chopping GD, Walker RG (1993) Northern dairy feedbase 2001. 4. Feeding systems during winter and spring. Tropical Grasslands 27, 180–211. open url image1

Kolver E, Muller LD, Varga GA, Cassidy TJ (1998) Synchronisation of ruminal degradation of supplemental carbohydrates with pasture nitrogen in lactating dairy cows. Journal of Dairy Science 81, 2017–2028.
PubMed |
open url image1

Minson DJ, McLeod MN (1972) The in vitro technique: its modification for estimating digestibility of large numbers of tropical pasture samples. CSIRO Division of Tropical Pastures Paper No. 8, Brisbane.

Nocek JE, Russell JB (1988) Protein and energy as an integrated system. Relationship of ruminal protein and carbohydrate availability to microbial synthesis and milk production. Journal of Dairy Science 71, 2070–2107. open url image1

Nocek JE, Hart SP, Polan CE (1987) Rumen ammonia concentration as influenced by storage time, freezing and thawing, acid preservative, and method of ammonia determination. Journal of Dairy Science 70, 601–607. open url image1

NRC (1985) ‘Ruminant nitrogen usage. Subcommittee on nitrogen usage in ruminants.’ (National Research Council, National Academic Press: Washington DC)

NRC (1989) ‘Nutrient requirements of dairy cattle.’ 6th edn. (National Research Council, National Academy Press: Washington DC)

NRC (2001) ‘Nutrient requirements of dairy cattle.’ 7th edn. (National Research Council, National Academy Press: Washington DC)

Ostrowski-Meissner HT (Ed.) (1987) ‘Australian feed composition tables. National collection (1970–1987).’ (AFIC–CSIRO: Sydney, Australia)

Orskov ER, McDonald I (1979) The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. The Journal of Agricultural Science 92, 499–503. open url image1

Reeves M, Fulkerson WJ, Kellaway RC, Dove H (1996) A comparison of three techniques to determine the herbage intake of dairy cows grazing kikuyu (Pennisetum clandestinum) pasture. Australian Journal of Experimental Agriculture 36, 23–30.
Crossref |
open url image1

Robertson JB, van Soest PJ (1981) The detergent system of analysis. In ‘The analysis of dietary fibre in food’. (Eds WPT James, O Theander) pp. 123 and 158. (Marcel Dekker: NY and Basel)

Ryan BF, Joiner BL, Ryan TA (1985) ‘Minitab handbook.’ 2nd edn. (PWS–KENT Publishing Company: Boston)

Satter LD, Slyter LL (1974) Effect of ammonia concentration on rumen microbial protein production in vitro. The British Journal of Nutrition 32, 199–208.
PubMed |
open url image1

SCA (1990) ‘Feeding standards for Australian livestock. Ruminants subcommittee.’ (Standing Committee on Agriculture, CSIRO Publications: East Melbourne, Australia)

Shaver RD, Nytes AJ, Satter LD, Jorgensen NA (1986) Influence of amount of feed intake and forage physical form on digestion and passage of prebloom alfalfa hay in dairy cows. Journal of Dairy Science 69, 1545–1559. open url image1

Sinclair LA, Garnsworthy PC, Newbold JR, Buttery PJ (1993) Effect of synchronizing the rate of dietary energy and nitrogen release on rumen fermentation and microbial protein synthesis in sheep. The Journal of Agricultural Science 120, 251–263. open url image1

Snedecor GW, Cochran WG (1967) ‘Statistical methods.’ (The Iowa State University Press: Ames, Iowa)

van Soest PJ, van Robertson JB, Lewis BA (1991) Methods of dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74, 3583–3597.
PubMed |
open url image1

Stobbs TH (1970) Automatic measurement of grazing time by dairy cows on tropical grass and legume pastures. Tropical Grasslands 4, 237–244. open url image1

USEPA (1997) Methods for the determination of metals in environmental samples, USEPA Method 200.7. United States Environmental Protection Agency, Washington DC.

Weatherburn MW (1967) Phenol-hypochlorite reaction for determination of ammonia. Analytical Chemistry 39, 971–974.
Crossref |
open url image1