Nitrogen fertiliser effects on nutritive characteristics of perennial ryegrass during late autumn, and mid- and late winter in western Victoria

Abstract

A study determined the effects of differing rates of nitrogen fertiliser [0 (N0), 25 (N1), 50 (N2) and 75 kg N/ha (N3)] during late autumn (T1) and mid- (T2) and late (T3) winter on the nutritive characteristics of perennial ryegrass over a 28-day period after each application. All nitrogen applications were made to pastures with a post-grazed residual mass (dry matter) of 1400 kg/ha. Changes in metabolisable energy followed similar patterns for all treatments within a given period. Metabolisable energy was highest in T1, ranging from 11.8 to 13.1 MJ/kg dry matter, followed by T2 (11.5-12.3 MJ/kg dry matter) and T3 (10.6-11.5 MJ/kg dry matter). Changes in crude protein for all treatments at each application time were similar, irrespective of rate of nitrogen application. At the commencement of treatment application times, the existing crude protein content (%DM) was highest in N3 (T1 19, T2 23, T3 22), followed by N2 (T1 18, T2 21, T3 21), N1 (T1 17, T2 20, T3 20) and N0 (T1 16, T2 17, T3 18). During both T1 and T2, neutral detergent fibre content decreased by 4 percentage units and increased by a similar amount during T3. Generally, neutral detergent fibre content (%DM) was highest during T3 (53-58%), followed by T2 (45-54%) and T1 (43-49%). Water-soluble carbohydrate content (%DM) increased during all treatment periods with the highest level observed during T1 (18-31%) followed by T2 (3-14%) and T3 (1-6%). Nitrate content (measured as nitrate-nitrogen) decreased throughout T1, primarily due to dry conditions, while during T2, levels for N3 and N2 were significantly (P<0.05) higher than for N1 and N0 following nitrogen fertiliser application. During T3, nitrate content increased for all treatments throughout the 28-day period, with highest nitrate levels being observed during T3. The effect of applied nitrogen on mineral content was variable within and across treatment periods. The study indicates that nitrogen fertiliser did not affect metabolisable (apart from N3 elevating metabolisable energy during T3), neutral detergent fibre or water-soluble carbohydrate contents of perennial ryegrass during the 28 days after nitrogen application, but increased crude protein content. Also, nitrogen fertiliser elevated nitrate content in perennial ryegrass. While the elevated nitrate content observed may result in subclinical effects, these levels are not considered fatal for dairy cows. Crude protein content was generally above 20% of dry matter throughout the study and close to 30% of dry matter for short periods during T2. Minimising the effect of excess nitrogen ingested by the grazing animal may require appropriate supplementation of low crude protein containing feeds such as cereal grains. It is argued that the effects of rain and temperature, which impact on soil nitrogen mineralisation, may have a greater influence on perennial ryegrass nitrate content than nitrogen fertiliser.