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Crop and Pasture Science Crop and Pasture Science Society
Plant sciences, sustainable farming systems and food quality
RESEARCH ARTICLE

Fate of fertiliser nitrogen in a ryegrass–kikuyu dairy pasture system

Michael Fitzgerald https://orcid.org/0000-0003-1605-3513 A * , Deirdre Harvey A , Johannes Friedl https://orcid.org/0000-0003-0468-916X B , David Rowlings B , Jason Condon C D and Warwick Dougherty A
+ Author Affiliations
- Author Affiliations

A NSW Department of Primary Industries, Elizabeth Macarthur Agricultural Institute, Menangle, NSW 2569, Australia.

B Institute for Future Environments, Queensland University of Technology, 2 George Street, Brisbane, Qld 4000, Australia.

C NSW Department of Primary Industries, Wagga Wagga Agricultural Institute, Wagga Wagga, NSW 2560, Australia.

D School of Agricultural, Environmental and Veterinary Sciences, Charles Sturt University, Wagga Wagga, NSW 2678, Australia.


Handling Editor: Caixian Tang

Crop & Pasture Science 74(12) 1272-1286 https://doi.org/10.1071/CP22410
Submitted: 6 January 2023  Accepted: 29 May 2023  Published: 22 June 2023

© 2023 The Author(s) (or their employer(s)). Published by CSIRO Publishing

Abstract

Context

Dairy pasture production is reliant on fertiliser to supply nitrogen (N); however, fertiliser N-use efficiency (FNUE) is low and N can be lost to the environment.

Aims

The aim of this study was to track the fate of N fertiliser applied in a pasture system of ryegrass (Lolium multiflorum, temperate grass) oversown into kikuyu (Pennisetum clandestinum, tropical grass).

Methods

We used 15N-labelled urea to track the residual plant uptake of a one-off application of 15N over three pasture cuts subsequent to the first cut in the kikuyu growing season from February 2018 to April 2018 (Experiment 1), followed by total soil and plant recoveries of 15N over a 12-month period (Experiment 2). Total N treatment rates were 0, 120, 240 and 480 kg N ha−1 year−1, consisting of application events of 40 kg N ha−1. In Experiment 1, 15N was applied only at the first fertilisation, whereas in Experiment 2, 15N-labelled urea was applied at each fertilisation event.

Key results

In Experiment 1, uptake of residual 15N fertiliser in the pasture biomass was <6%. In Experiment 2, FNUE was 29–32% and unaccounted 15N fertiliser was 22–142 kg N ha−1, across the 120, 240 and 480 kg N ha−1 year−1 treatments.

Conclusions

Recovery of 15N residual fertiliser did not increase with N rate and was attributed to the mass increase in soil 15N recovery. FNUE in the pasture did not decrease with N rate. Unaccounted 15N increased with N rate.

Implications

Existing and alternative N and pasture management strategies such as clover and multi-species pasture need to be implemented and explored to reduce the amount of unaccounted N in dairy pasture production.

Keywords: dairy systems, efficiency, New South Wales, Nitrogen-15, pasture production, profitability, soil-plant interaction, sustainability.

References

Benbrook CM, Davis DR, Heins BJ, Latif MA, Leifert C, Peterman L, Butler G, Faergeman O, Abel-Caines S, Baranski M (2018) Enhancing the fatty acid profile of milk through forage-based rations, with nutrition modeling of diet outcomes. Food Science & Nutrition 6, 681-700.
| Crossref | Google Scholar |

Billingham K (2015) Nitrogen mineralisation and availability under kikuyu dairy pastures on the Mid-North Coast of NSW. Dairy Australia, Melbourne, Vic., Australia. Available at http://www.dairyingfortomorrow.com.au/wp-content/uploads/Literature-review-Nitrogen-mineralisation-and-availability-under-kikuyu.pdf%0Ahttp://www.dairyingfortomorrow.com.au/tackling-specific-issues/soils/trial/

Bilotto F, Harrison MT, Migliorati MDA, Christie KM, Rowlings DW, Grace PR, Smith AP, Rawnsley RP, Thorburn PJ, Eckard RJ (2021) Can seasonal soil N mineralisation trends be leveraged to enhance pasture growth? Science of The Total Environment 772,.
| Crossref | Google Scholar |

Box LA, Edwards GR, Bryant RH (2017) Milk production and urinary nitrogen excretion of dairy cows grazing plantain in early and late lactation. New Zealand Journal of Agricultural Research 60, 470-482.
| Crossref | Google Scholar |

Bryant RH, Snow VO, Shorten PR, Welten BG (2020) Can alternative forages substantially reduce N leaching? findings from a review and associated modelling. New Zealand Journal of Agricultural Research 63, 3-28.
| Crossref | Google Scholar |

Bureau of Meteorology (2020) Climate statistics for Australian locations. Monthly climate statistics. Summary statistics Camden airport. pp 45-47 Available at http://www.bom.gov.au/climate/averages/tables/cw_068192_All.shtml [Verified 17 March 2023].
| Google Scholar |

Burkitt LL (2014) A review of nitrogen losses due to leaching and surface runoff under intensive pasture management in Australia. Soil Research 52, 621-636.
| Crossref | Google Scholar |

Carran RA, Clough T (1996) Environmental impacts of nitrogen in pastoral agriculture. In ‘White clover: New Zealand’s competitive edge proceedings joint symposium Agronomy Society of New Zealand and New Zealand Grassland Association’. Lincoln University, New Zealand, 21–22 November 1995. pp. 99–102. (New Zealand Grassland Association)

Catchpoole VR, Oxenham DJ, Harper LA (1983) Transformation and recovery of urea applied to a grass pasture in south-eastern Queensland. Australian Journal of Experimental Agriculture 23, 80-86.
| Crossref | Google Scholar |

Chapman D, Pinxterhuis I, Ledgard S, Parsons T, Chapman D, Pinxterhuis I, Ledgard S, Parsons T (2020) White clover or nitrogen fertiliser for dairying under nitrate leaching limits? Animal Production Science 60, 78-83.
| Crossref | Google Scholar |

Chen D, Lam SK, Mosier AR, Eckard R, Vitousek P (2016) Should soil nitrogen be mined? In ‘Proceedings of the 2016 International Nitrogen Initiative Conference, Solutions to improve nitrogen use efficiency for the world’. Available at www.ini2016.com [Verified 1 April 2023]

Christie KM, Smith AP, Rawnsley RP, Harrison MT, Eckard RJ (2018) Simulated seasonal responses of grazed dairy pastures to nitrogen fertilizer in SE Australia: pasture production. Agricultural Systems 166, 36-47.
| Crossref | Google Scholar |

Cookson WR, Rowarth JS, Cameron KC (2000) The fate of residual nitrogen fertiliser applied to a ryegrass (Lolium perenne L.) seed crop. Australian Journal of Agricultural Research 51, 287-294.
| Crossref | Google Scholar |

Cranston LM, Kenyon PR, Morris ST, Lopez-Villalobos N, Kemp PD (2016) Morphological and physiological responses of plantain (Plantago lanceolata) and chicory (Cichorium intybus) to water stress and defoliation frequency. Journal of Agronomy and Crop Science 202, 13-24.
| Crossref | Google Scholar |

Cullen BR, Eckard RJ, Callow MN, Johnson IR, Chapman DF, Rawnsley RP, Garcia SC, White T, Snow VO (2008) Simulating pasture growth rates in Australian and New Zealand grazing systems. Australian Journal of Agricultural Research 59, 761-768.
| Crossref | Google Scholar |

Dairy Australia (2013) ‘Dairy soils and fertiliser manual: Australian nutrient management guidelines.’ (Dairy Australia: Melbourne, Vic., Australia)

Dairy Australia (2018) ‘Best management practices for nitrogen use on dairy pastures.’ (Dairy Australia: Melbourne, Vic., Australia)

Dairy Australia (2021a) NSW dairy farm monitor project 2020/2021. Dairy Australia, Melbourne, Vic., Australia. Available at https://www.dairyaustralia.com.au/industry-statistics/dairy-farm-monitor-project#.ZBBqbHZByUl. [Verified 14 March 2023]

Dairy Australia (2021b) Dairy farm monitor project. Dairy Australia, Melbourne, Vic., Australia. Available at https://www.dairyaustralia.com.au/industry-statistics/dairy-farm-monitor-project#.ZCfA5nZBw2w [Verified 1 April 2023]

de Klein CAM, Monaghan RM, Alfaro M, Gourley CJP, Oenema O, Powell JM (2017) Nitrogen performance indicators for dairy production systems. Soil Research 55, 479-488.
| Crossref | Google Scholar |

Dhamala NR, Rasmussen J, Carlsson G, Søegaard K, Eriksen J (2018) Effects of including forbs on N2-fixation and N yield in red clover-ryegrass mixtures. Plant and Soil 424, 525-537.
| Crossref | Google Scholar |

Distel RA, Arroquy JI, Lagrange S, Villalba JJ (2020) Designing diverse agricultural pastures for improving ruminant production systems. Frontiers in Sustainable Food Systems 4, 596869.
| Crossref | Google Scholar |

Dougherty WJ, Collins D, Van Zwieten L, Rowlings DW (2016) Nitrification (DMPP) and urease (NBPT) inhibitors had no effect on pasture yield, nitrous oxide emissions, or nitrate leaching under irrigation in a hot-dry climate. Soil Research 54, 675-683.
| Crossref | Google Scholar |

Eckard R (2006) Nitrogen-growth promotant for pastures. In ‘Proceedings of the 47th annual conference of the Grassland Society of Southern Australia’. pp. 31–42. (Grassland Society of Southern Australia: Warragul, Vic., Australia)

Eckard RJ, Clark H (2020) Potential solutions to the major greenhouse-gas issues facing Australasian dairy farming. Animal Production Science 60, 10-16.
| Crossref | Google Scholar |

Eckard RJ, Chapman DF, White RE, Chen D (2004) The environmental impact of nitrogen fertiliser use on dairy pastures. Australian Journal of Dairy Technology 59, 145-148.
| Google Scholar |

Eckard RJ, Chapman DF, White RE (2007) Nitrogen balances in temperate perennial grass and clover dairy pastures in south-eastern Australia. Australian Journal of Agricultural Research 58, 1167-1173.
| Crossref | Google Scholar |

FAO (2018) Chapter 7. Dairy and dairy products. In ‘OECD-FAO Agricultural Outlook 2018–2027’. pp. 163–174. (FAO: Rome, Italy)

Friedl J, Scheer C, Rowlings DW, Mumford MT, Grace PR (2017) The nitrification inhibitor DMPP (3,4-dimethylpyrazole phosphate) reduces N2 emissions from intensively managed pastures in subtropical Australia. Soil Biology and Biochemistry 108, 55-64.
| Crossref | Google Scholar |

Fulkerson B (2007) ‘Kikuyu grass (Pennisetum clandestinum).’ (Future Dairy, University of Sydney: Camden, NSW, Australia)

Gourley CJP, Dougherty WJ, Weaver DM, Aarons SR, Awty IM, Gibson DM, Hannah MC, Smith AP, Peverill KI (2012) Farm-scale nitrogen, phosphorus, potassium and sulfur balances and use efficiencies on Australian dairy farms. Animal Production Science 52, 929-944.
| Crossref | Google Scholar |

Henzell EF (1971) Recovery of nitrogen from four fertilizers applied to Rhodes grass in small plots. Australian Journal of Experimental Agriculture 11, 420-430.
| Crossref | Google Scholar |

IAEA (2001) ‘Use of isotope and radiation methods in soil and water management and crop nutrition.’ Training Course Series No. 14. (International Atomic Energy Agency: Vienna, Austria)

Isbell RF (1996) ‘The Australian Soil Classification.’ (CSIRO Publishing: Melbourne, Vic., Australia) 10.1016/s0016-7061(96)00096-1

Jenkinson DS, Fox RH, Rayner JH (1985) Interactions between fertilizer nitrogen and soil nitrogen-the so-called ‘priming’ effect. Journal of Soil Science 36, 425-444.
| Crossref | Google Scholar |

Ledgard SF, Penno JW, Sprosen MS (1999) Nitrogen inputs and losses from clover/grass pastures grazed by dairy cows, as affected by nitrogen fertilizer application. The Journal of Agricultural Science 132, 215-225.
| Crossref | Google Scholar |

Liu W, Yang H, Liu J, Azevedo LB, Wang X, Xu Z, Abbaspour KC, Schulin R (2016) Global assessment of nitrogen losses and trade-offs with yields from major crop cultivations. Science of The Total Environment 572, 526-537.
| Crossref | Google Scholar |

Lowe KF, Fulkerson WJ, Walker RG, Armour JD, Bowdler TM, Slack K, Knight RI, Moody PW, Pepper PM (2005) Comparative productivity of irrigated short-term ryegrass (Lolium multiflorum) pasture receiving nitrogen, grown alone or in a mixture with white (Trifolium repens) and Persian (T. resupinatum) clovers. Australian Journal of Experimental Agriculture 45, 21-39.
| Crossref | Google Scholar |

Lüscher A, Mueller-Harvey I, Soussana JF, Rees RM, Peyraud JL (2014) Potential of legume-based grassland-livestock systems in Europe: a review. Grass and Forage Science 69, 206-228.
| Crossref | Google Scholar |

Mccarthy S, Wims C, Lee J (2016) ‘Ryegrass-spring grazing management paddock guide.’ (Dairy Australia: Melbourne, Vic., Australia). Available at https://www.dairyaustralia.com.au/resource-repository/2020/07/09/ryegrass-spring-grazing-management-paddock-guide#.ZCjs_nZBw2w [Verified 2 April 2023]

McDowell RW, Rotz CA, Oenema J, Macintosh KA (2022) Limiting grazing periods combined with proper housing can reduce nutrient losses from dairy systems. Nature Food 3(12), 1065-1074.
| Crossref | Google Scholar |

McKenzie N, Coughlan K, Cresswell H (2002) ‘Soil physical measurement and interpretation for land evaluation.’ (CSIRO Publishing: Melbourne, Vic., Australia)

McNally SR, Laughlin DC, Rutledge S, Dodd MB, Six J, Schipper LA (2015) Root carbon inputs under moderately diverse sward and conventional ryegrass-clover pasture: implications for soil carbon sequestration. Plant and Soil 392, 289-299.
| Crossref | Google Scholar |

Moir JL, Edwards GR, Berry LN (2013) Nitrogen uptake and leaching loss of thirteen temperate grass species under high N loading. Grass and Forage Science 68, 313-325.
| Crossref | Google Scholar |

Mudge PL, Kelliher FM, Knight TL, O’Connell D, Fraser S, Schipper LA (2017) Irrigating grazed pasture decreases soil carbon and nitrogen stocks. Global Change Biology 23, 945-954.
| Crossref | Google Scholar |

Mulvaney RL, Khan SA, Ellsworth TR (2009) Synthetic nitrogen fertilizers deplete soil nitrogen: a global dilemma for sustainable cereal production. Journal of Environmental Quality 38, 2295-2314.
| Crossref | Google Scholar |

Oenema O, Brentrup F, Lammel J, Bascou P, Billen G, Dobermann A, Erisman JW, Garnett T, Hammel M, Haniotis T, Hillier J, Hoxha A, Jensen LS, Oleszek W, Pallière C, Powlson D, Quemada M, Schulman M, Sutton MA, Van Grinsven HJM, Winiwarter W (2015) Nitrogen use efficiency (NUE)-an indicator for the utilization of nitrogen in agriculture and food systems. Prepared by EU Nitrogen Expert Panel. Available at https://www.ipipotash.org/uploads/udocs/nue-indicator-peport-nitrogen-expert-panel-18-12-2015.pdf [Verified 11 June 2023]

Pembleton KG, Tozer KN, Edwards GR, Jacobs JL, Turner LR (2015) Simple versus diverse pastures: opportunities and challenges in dairy systems. Animal Production Science 55, 893-901.
| Crossref | Google Scholar |

Pembleton KG, Hills JL, Freeman MJ, McLaren DK, French M, Rawnsley RP (2016) More milk from forage: milk production, blood metabolites, and forage intake of dairy cows grazing pasture mixtures and spatially adjacent monocultures. Journal of Dairy Science 99, 3512-3528.
| Crossref | Google Scholar |

Rabobank (2020) Southeast Asia beckons – dairy exports into ASEAN-6 nations set to boom over next decade. pp. 4–7. Available at https://www.rabobank.com.au/media-releases/2020/201015-southeast-asia-beckons-dairy-exports-into-asean-6-nations-set-to-boom-over-next-decade/ [Verified 16 November 2020]

Rabobank (2022) The opportunities and challenges of de-intensifying your dairy system. Smash Trust, Cambridge, New Zealand. Available at https://www.smallerherds.co.nz/knowledge-hub/feed/the-opportunities-and-challenges-of-de-intensifying-your-dairy-system/ [Verified 2 January 2021]

Rao ACS, Smith JL, Papendick RI, Parr JF (1991) Influence of added nitrogen interactions in estimating recovery efficiency of labeled nitrogen. Soil Science Society of America Journal 55, 1616-1621.
| Crossref | Google Scholar |

Rawnsley RP, Smith AP, Christie KM, Harrison MT, Eckard RJ (2019) Current and future direction of nitrogen fertiliser use in Australian grazing systems. Crop & Pasture Science 70, 1034-1043.
| Crossref | Google Scholar |

Rayment GE, Lyons DJ (2010) ‘Soil chemical methods – Australasia.’ (CSIRO Publishing: Melbourne, Vic., Australia) doi:10.1071/9780643101364

Reeves M, Fulkerson WJ (1996) Establishment of an optimal grazing time of kikuyu pastures for dairy cows. In ‘Proceedings of the 8th Australian agronomy conference, The University of Southern Queensland, Toowoomba, Queensland 30 January−2 February’. (Australian Society of Agronomy)

Reuter DJ, Robinson JB (1997) ‘Plant analysis: an interpretation manual’. p. 572. (CSIRO Publishing: Melbourne, Vic., Australia). Available at https://www.publish.csiro.au/book/437 [Verified 25 March 2023]

Roche JR, Berry DP, Bryant AM, Burke CR, Butler ST, Dillon PG, Donaghy DJ, Horan B, Macdonald KA, Macmillan KL (2017) A 100-Year review: a century of change in temperate grazing dairy systems. Journal of Dairy Science 100, 10189-10233.
| Crossref | Google Scholar |

Rowlings DW, Scheer C, Liu S, Grace PR (2016) Annual nitrogen dynamics and urea fertilizer recoveries from a dairy pasture using 15N; effect of nitrification inhibitor DMPP and reduced application rates. Agriculture, Ecosystems & Environment 216, 216-225.
| Crossref | Google Scholar |

Scheer C, Rütting T (2023) Use of 15N tracers to study nitrogen flows in agro-ecosystems: transformation, losses and plant uptake. Nutrient Cycling in Agroecosystems 125, 89-93.
| Crossref | Google Scholar |

Scheer C, Grace PR, Rowlings DW, Kimber S, Van Zwieten L (2011) Effect of biochar amendment on the soil-atmosphere exchange of greenhouse gases from an intensive subtropical pasture in northern New South Wales, Australia. Plant and Soil 345, 47-58.
| Crossref | Google Scholar |

Smedman A, Lindmark-Månsson H, Drewnowski A, Edman A-KM (2010) Nutrient density of beverages in relation to climate impact. Food & Nutrition Research 54, 5170.
| Crossref | Google Scholar |

Smith AP, Christie KM, Rawnsley RP, Eckard RJ (2018) Fertiliser strategies for improving nitrogen use efficiency in grazed dairy pastures. Agricultural Systems 165, 274-282.
| Crossref | Google Scholar |

Smith AP, Beale P, Fulkerson BJ, Eckard RJ (2019) Managing the nitrogen status of subtropical dairy pastures for production, efficiency and profit. Agricultural Systems 176, 102677.
| Crossref | Google Scholar |

Staines MVH, Morris RJ, Bolland MDA, McDonnell RP (2018) Performance of five pasture-based dairy systems with increasing levels of nitrogen fertiliser and associated stocking rates. New Zealand Journal of Agricultural Research 61, 1-26.
| Crossref | Google Scholar |

Suter H, Sultana H, Turner D, Davies R, Walker C, Chen D (2013) Influence of urea fertiliser formulation, urease inhibitor and season on ammonia loss from ryegrass. Nutrient Cycling in Agroecosystems 95, 175-185.
| Crossref | Google Scholar |

Suter H, Belyaeva O, Ward G, Li Y (2018) Nitrogen mineralisation and impacts on fertiliser efficiency in pastures. Motivation for Research Program. (Agronomy Community Forums, Nutrient Advantage: Werribee, Victoria Australia) Available at https://www.nutrientadvantage.com.au/~/media/Files/NA/AgronomyCommunity/Suter_IPF Agronomy meeting_July_2018v2.pdf

Suter H, Lam SK, Walker C, Chen D (2020) Enhanced efficiency fertilisers reduce nitrous oxide emissions and improve fertiliser 15N recovery in a Southern Australian pasture. Science of The Total Environment 699, 134147.
| Crossref | Google Scholar |

Totty VK, Greenwood SL, Bryant RH, Edwards GR (2013) Nitrogen partitioning and milk production of dairy cows grazing simple and diverse pastures. Journal of Dairy Science 96, 141-149.
| Crossref | Google Scholar |

Unkovich M (2012) Nitrogen fixation in Australian dairy systems: review and prospect. Crop & Pasture Science 63, 787-804.
| Crossref | Google Scholar |

Van Groenigen JW, Velthof GL, Oenema O, Van Groenigen KJ, Van Kessel C (2010) Towards an agronomic assessment of N2O emissions: a case study for arable crops. European Journal of Soil Science 61, 903-913.
| Crossref | Google Scholar |

Weier KL, Grace PR (2012) ‘A review of denitrification on Australian dairy farms.’ (Dairy Australia: Melbourne, Vic., Australia)

Whitehead DC (1986) Sources and transformations of organic nitrogen in intensively managed grassland soils. In ‘Nitrogen fluxes intensive grassland systems’. (Eds HG Van Der Meer, JC Ryden, GC Ennik) pp. 47–58. (Springer: Dordrecht, Netherlands) doi:10.1007/978-94-009-4394-0_5

Wilkinson JM, Lee MRF, Rivero MJ, Chamberlain AT (2020) Some challenges and opportunities for grazing dairy cows on temperate pastures. Grass and Forage Science 75, 1-17.
| Crossref | Google Scholar |