Register      Login
Crop and Pasture Science Crop and Pasture Science Society
Plant sciences, sustainable farming systems and food quality
RESEARCH ARTICLE

Nitrogen fertilisation affected zinc and selenium biofortification in silage maize

Klara Petković https://orcid.org/0000-0002-3784-3736 A , Maja Manojlović A , Ranko Čabilovski https://orcid.org/0000-0001-9798-9908 A * , Zdenko Lončarić https://orcid.org/0000-0003-4927-3969 B , Đorđe Krstić A , Dragan Kovačević https://orcid.org/0000-0001-9602-088X A and Marko Ilić C
+ Author Affiliations
- Author Affiliations

A Faculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovića 8, 21000 Novi Sad, Serbia.

B Faculty of Agrobiotechnical Sciences Osijek, Josip Juraj Strossmayer University of Osijek, Vladimira Preloga 1, 31000 Osijek, Croatia.

C Faculty of Technology Novi Sad, University of Novi Sad, Bulevar Cara Lazara 1, 21000, Novi Sad, Serbia.

* Correspondence to: ranko.cabilovski@polj.uns.ac.rs

Handling Editor: Shahid Hussain

Crop & Pasture Science - https://doi.org/10.1071/CP21735
Submitted: 30 June 2021  Accepted: 26 November 2021   Published online: 13 May 2022

© 2022 The Author(s) (or their employer(s)). Published by CSIRO Publishing

Abstract

Context: Biofortification of forage crops has become even more important, due to the improvement in livestock nutrition, but it has also had an indirect positive impact on the human diet.

Aim: This study investigated the effect of nitrogen and microelement (Zn and Se) fertilisation on yield and on the microelement composition of maize (Zea mays L.) silage.

Methods: Two field experiments were conducted using a two-factorial split-plot design with nitrogen fertilisation in three doses: 0, 120, 180 and 240 kg N/ha. The first experiment included foliar Zn fertilisation as the second factor (0, 1.5 kg Zn/ha and 1.5 kg Zn/ha + urea solution). The second experiment studied the effect of Se (10 g Se/ha).

Key results: Nitrogen fertilisation increased biomass yield, Cu and Mn concentration in silage maize. Application of Se and Zn did not affect the biomass yield, but it had a positive effect on Se and Zn concentration in plants. Zn and urea application in combination proved to be more efficient in increasing Zn concentration in plants when compared to Zn applied alone.

Conclusions: Nitrogen and fertilisation with Zn and Se can be a good tool in fodder plant biofortification because their application led to a yield increase (Zn), but at the same time to an improvement in the mineral composition of maize biomass, with essential elements (Zn and Se).

Implications: Although biofortification with 1.5 kg Zn/ha has achieved the concentration in maize biomass that can meet the nutritional needs of dairy cows, further research is needed to examine the adjuvant doses and forms of Zn to obtain high yields and Zn concentration in forage crops.

Keywords: forage management, micronutrients, mineral composition, ruminant nutrition, silage.


References

Ahmad R, Waraich EA, Ashraf MY, Ahmad S, Aziz T (2014) Does nitrogen fertilization enhance drought tolerance in sunflower? A review. Journal of Plant Nutrition 37, 942–963.
Does nitrogen fertilization enhance drought tolerance in sunflower? A review.Crossref | GoogleScholarGoogle Scholar |

Bukvić G, Antunović M, Popović S, Rastija M (2003) Effect of P and Zn fertilisation on biomass yield and its uptake by maize lines (Zea mays L.). Plant, Soil and Environment 49, 505–510.
Effect of P and Zn fertilisation on biomass yield and its uptake by maize lines (Zea mays L.).Crossref | GoogleScholarGoogle Scholar |

Cakmak I, Kutman UB (2018) Agronomic biofortification of cereals with zinc: a review. European Journal of Soil Science 69, 172–180.
Agronomic biofortification of cereals with zinc: a review.Crossref | GoogleScholarGoogle Scholar |

Cakmak I, Pfeiffer WH, McClafferty B (2010) Review: Biofortification of durum wheat with zinc and iron. Cereal Chemistry 87, 10–20.
Review: Biofortification of durum wheat with zinc and iron.Crossref | GoogleScholarGoogle Scholar |

Chaudhary DP, Jat SL, Kumar R, Kumar A, Kumar B (2014) Fodder quality of maize: its preservation. In ‘Maize: nutrition dynamics and novel uses’. (Eds DP Chaudhary, S Kumar, S Langyan) pp. 153–160. (Springer: New Delhi, India)

Das S, Green A (2016) Zinc in crops and human health. In ‘Biofortification of food crops’. (Eds U Singh, CS Praharaj, SS Singh, NP Singh) pp. 31–40. (Springer: New Delhi, India)

Dinh QT, Wang M, Tran TAT, Zhou F, Wang D, Zhai H, Peng Q, Xue M, Du Z, Bañuelos GS, Lin Z-Q, Liang D (2019) Bioavailability of selenium in soil-plant system and a regulatory approach. Critical Reviews in Environmental Science and Technology 49, 443–517.
Bioavailability of selenium in soil-plant system and a regulatory approach.Crossref | GoogleScholarGoogle Scholar |

Dynes RA, Henry DA, Masters DG (2003) Characterising forages for ruminant feeding. Asian-Australasian Journal of Animal Sciences 16, 116–123.
Characterising forages for ruminant feeding.Crossref | GoogleScholarGoogle Scholar |

Dzomba E, Djikic M, Gadzo D, Cengic-Dzomba S, Loncaric Z, Singh BR (2018) Effect of different doses and application methods of sodium selenate on selenium status in maize for silage. Agricultural and Food Science 27, 255–263.
Effect of different doses and application methods of sodium selenate on selenium status in maize for silage.Crossref | GoogleScholarGoogle Scholar |

Egner H, Riehm H, Domingo WR (1960) Investigations of the chemical soil analysis as a basis for the evaluation of nutrient status in soil. II. Chemical extraction methods for phosphorus and potassium determination. Kungliga Lantbrukshügskolans Annaler 26, 195–215.

Fahad S, Hussain S, Saud S, Hassan S, Shan D, Chen Y, Deng N, Khan F, Wu C, Wu W, Shah F, Ullah B, Yousaf M, Ali S, Huang J (2015) Grain cadmium and zinc concentrations in maize influenced by genotypic variations and zinc fertilization. Clean – Soil, Air, Water 43, 1433–1440.
Grain cadmium and zinc concentrations in maize influenced by genotypic variations and zinc fertilization.Crossref | GoogleScholarGoogle Scholar |

Feil B, Moser SB, Jampatong S, Stamp P (2005) Mineral composition of the grains of tropical maize varieties as affected by pre-anthesis drought and rate of nitrogen fertilization. Crop Science 45, 516–523.
Mineral composition of the grains of tropical maize varieties as affected by pre-anthesis drought and rate of nitrogen fertilization.Crossref | GoogleScholarGoogle Scholar |

Fernández V, Sotiropoulos T, Brown PH (2013) ‘Foliar fertilization: scientific principles and field practices’, (International Fertilizer Industry Association: Paris, France)

Fixen P, Brentrup F, Bruulsema T, Garcia F, Norton R, Zingore S (2015) Nutrient/fertilizer use efficiency: measurement, current situation and trends. In ‘Managing water and fertilizer for sustainable agricultural intensification’. (Eds P Drechsel, P Heffer, H Magen, R Mikkelsen, D Wichelns) pp. 8–37. (International Fertilizer Industry Association: Paris, France)

Fordyce FM (2013) Selenium deficiency and toxicity in the environment. In ‘Essentials of medical geology’. (Ed. O Selinus) pp. 375–416. (Springer: Dordrecht, Netherlands)

Grujcic D, Hansen TH, Husted S, Drinic M, Singh BR (2018) Effect of nitrogen and zinc fertilization on zinc and iron bioavailability and chemical speciation in maize silage. Journal of Trace Elements in Medicine and Biology 49, 269–275.
Effect of nitrogen and zinc fertilization on zinc and iron bioavailability and chemical speciation in maize silage.Crossref | GoogleScholarGoogle Scholar | 29449108PubMed |

Grujcic D, Yazici AM, Tutus Y, Cakmak I, Singh BR (2021) Biofortification of silage maize with zinc, iron and selenium as affected by nitrogen fertilization. Plants 10, 391
Biofortification of silage maize with zinc, iron and selenium as affected by nitrogen fertilization.Crossref | GoogleScholarGoogle Scholar | 33670608PubMed |

Hall JA, Bobe G, Hunter JK, Vorachek WR, Stewart WC, Vanegas JA, Estill CT, Wayne D, Mosher WD, Pirelli GJ (2013) Effect of feeding selenium-fertilized alfalfa hay on performance of weaned beef calves. PLoS ONE 8, e58188
Effect of feeding selenium-fertilized alfalfa hay on performance of weaned beef calves.Crossref | GoogleScholarGoogle Scholar | 23536788PubMed |

Hamnér K, Weih M, Eriksson J, Kirchmann H (2017) Influence of nitrogen supply on macro- and micronutrient accumulation during growth of winter wheat. Field Crops Research 213, 118–129.
Influence of nitrogen supply on macro- and micronutrient accumulation during growth of winter wheat.Crossref | GoogleScholarGoogle Scholar |

Hasanuzzaman M, Bhuyan MHMB, Raza A, Hawrylak-Nowak B, Matraszek-Gawron R, Mahmud JA, Nahar K, Fujita M (2020) Selenium in plants: boon or bane? Environmental and Experimental Botany 178, 104170
Selenium in plants: boon or bane?Crossref | GoogleScholarGoogle Scholar |

Imran M, Rehim A (2017) Zinc fertilization approaches for agronomic biofortification and estimated human bioavailability of zinc in maize grain. Archives of Agronomy and Soil Science 63, 106–116.
Zinc fertilization approaches for agronomic biofortification and estimated human bioavailability of zinc in maize grain.Crossref | GoogleScholarGoogle Scholar |

ISO International Organization for Standardization (2005) ‘ISO 10693:2005, Soil quality determination of carbonate content volumetric method,’ (International Organization for Standardization: Geneva, Switzerland)

Jaaf S, Batty B, Krueger A, Estill CT, Bionaz M (2020) Selenium biofortified alfalfa hay fed in low quantities improves selenium status and glutathione peroxidase activity in transition dairy cows and their calves. Journal of Dairy Research 87, 184–190.
Selenium biofortified alfalfa hay fed in low quantities improves selenium status and glutathione peroxidase activity in transition dairy cows and their calves.Crossref | GoogleScholarGoogle Scholar | 32295653PubMed |

Kalra Y (1997) ‘Handbook of reference methods for plant analysis’, (CRC Press: Boca Raton, FL, USA)

Keskin B, Akdeniz H, Yilmaz IH, Turan N (2005) Yield and quality of forage corn (Zea mays L.) as influenced by cultivar and nitrogen rate. Journal of Agronomy 4, 138–141.
Yield and quality of forage corn (Zea mays L.) as influenced by cultivar and nitrogen rate.Crossref | GoogleScholarGoogle Scholar |

Kieliszek M (2019) Selenium–fascinating microelement, properties and sources in food. Molecules 24, 1298
Selenium–fascinating microelement, properties and sources in food.Crossref | GoogleScholarGoogle Scholar |

Kutman UB, Yildiz B, Ozturk L, Cakmak I (2010) Biofortification of durum wheat with zinc through soil and foliar applications of nitrogen. Cereal Chemistry 87, 1–9.
Biofortification of durum wheat with zinc through soil and foliar applications of nitrogen.Crossref | GoogleScholarGoogle Scholar |

Kutman UB, Yildiz B, Cakmak I (2011) Effect of nitrogen on uptake, remobilization and partitioning of zinc and iron throughout the development of durum wheat. Plant and Soil 342, 149–164.
Effect of nitrogen on uptake, remobilization and partitioning of zinc and iron throughout the development of durum wheat.Crossref | GoogleScholarGoogle Scholar |

Lindsay WL, Norvell WA (1978) Development of a DTPA soil test for zinc, iron, manganese, and copper. Soil Science Society of America Journal 42, 421–428.
Development of a DTPA soil test for zinc, iron, manganese, and copper.Crossref | GoogleScholarGoogle Scholar |

Liu D-Y, Zhang W, Yan P, Chen X-P, Zhang F-S, Zou C-Q (2017) Soil application of zinc fertilizer could achieve high yield and high grain zinc concentration in maize. Plant and Soil 411, 47–55.
Soil application of zinc fertilizer could achieve high yield and high grain zinc concentration in maize.Crossref | GoogleScholarGoogle Scholar |

Losak T, Hlusek J, Martinec J, Jandak J, Szostkova M, Filipcik R, Manasek J, Prokes K, Peterka J, Varga L, Ducsay L, Orosz F, Martensson A (2011) Nitrogenfertilization does not affect micronutrient uptake in grain maize (Zea mays L.). Acta Agriculturae Scandinavica, Section B — Soil & Plant Science 61, 543–550.
Nitrogenfertilization does not affect micronutrient uptake in grain maize (Zea mays L.).Crossref | GoogleScholarGoogle Scholar |

Lyons G, Cakmak I (2012) Agronomic biofortification of food crops with micronutrients. In ‘Fertilizing crops to improve human health: a scientific review. Vol. 1’. (Eds TW Bruulsema, P Heffer, RM Welch, I Cakmak, K Moran) pp. 97–122. (International Plant Nutrition Institute)

Manojlović MS, Lončarić Z, Cabilovski RR, Popović B, Karalić K, Ivezić V, Ademi A, Singh BR (2019) Biofortification of wheat cultivars with selenium. Acta Agriculturae Scandinavica, Section B – Soil & Plant Science 69, 715–724.
Biofortification of wheat cultivars with selenium.Crossref | GoogleScholarGoogle Scholar |

National Research Council (2001) ‘Nutrient requirements of dairy cows’, (The National Academies Press: Washington, DC, USA)

National Research Council (2005) ‘Mineral tolerance of animals’, (National Academies Press: Washington, DC, USA)

Nieder R, Benbi DK, Reichl FX (2018) ‘Soil components and human health’, (Springer: Berlin)
| Crossref |

Pavlovic Z, Miletic I, Zekovic M, Nikolic M, Glibetic M (2018) Impact of selenium addition to animal feeds on human selenium status in Serbia. Nutrients 10, 225
Impact of selenium addition to animal feeds on human selenium status in Serbia.Crossref | GoogleScholarGoogle Scholar |

Petković K, Manojlović M, Lombnæs P, Čabilovski R, Krstıć Đ, Lončarić Z, Lombnæs P (2019) Foliar application of selenium, zinc and copper in alfalfa (Medicago sativa L.) biofortification. Turkish Journal of Field Crops 24, 81–90.
Foliar application of selenium, zinc and copper in alfalfa (Medicago sativa L.) biofortification.Crossref | GoogleScholarGoogle Scholar |

Popovic D, Bozic T, Stevanovic J, Frontsyeva M, Todorovic D, Ajtic J, Spacic Jokic V (2010) Concentration of trace elements in blood and feed of homebred animals in Southern Serbia. Environmental Science and Pollution Research 17, 1119–1128.
Concentration of trace elements in blood and feed of homebred animals in Southern Serbia.Crossref | GoogleScholarGoogle Scholar | 20012897PubMed |

Potarzycki J, Grzebisz W (2009) Effect of zinc foliar application on grain yield of maize and its yielding component. Plant, Soil and Environment 55, 519–527.
Effect of zinc foliar application on grain yield of maize and its yielding component.Crossref | GoogleScholarGoogle Scholar |

Reynolds-Marzal MD, Rivera-Martín AM, Rodrigo SM, Santamaria O, Poblaciones MJ (2021) Biofortification of forage peas with combined application of selenium and zinc under Mediterranean conditions. Journal of Soil Science and Plant Nutrition 21, 286–300.
Biofortification of forage peas with combined application of selenium and zinc under Mediterranean conditions.Crossref | GoogleScholarGoogle Scholar |

Rojas LX, McDowell LR, Cousins RJ, Martin FG, Wilkinson NS, Johnson AB, Velasquez JB (1995) Relative bioavailability of two organic and two inorganic zinc sources fed to sheep. Journal of Animal Science 73, 1202–1207.
Relative bioavailability of two organic and two inorganic zinc sources fed to sheep.Crossref | GoogleScholarGoogle Scholar | 7628965PubMed |

Roohani N, Hurrell R, Kelishadi R, Schulin R (2013) Zinc and its importance for human health: an integrative review. Journal of Research in Medical Sciences 18, 144

Séboussi R, Tremblay GF, Ouellet V, Chouinard PY, Chorfi Y, Bélanger G, Charbonneau É (2016) Selenium-fertilized forage as a way to supplement lactating dairy cows. Journal of Dairy Science 99, 5358–5369.
Selenium-fertilized forage as a way to supplement lactating dairy cows.Crossref | GoogleScholarGoogle Scholar | 27085399PubMed |

Skrajnowska D, Bobrowska-Korczak B (2019) Role of zinc in immune system and anti-cancer defense mechanisms. Nutrients 11, 2273
Role of zinc in immune system and anti-cancer defense mechanisms.Crossref | GoogleScholarGoogle Scholar |

Smoleń S, Skoczylas Ł, Rakoczy R, Ledwożyw-Smoleń I, Kopeć A, Piątkowska E, Bieżanowska-Kopeć R, Pysz M, Koronowicz A, Kapusta-Duch J, Sady W (2015) Mineral composition of field-grown lettuce (Lactuca sativa L.) depending on the diversified fertilization with iodine and selenium compounds. Acta Scientarium Polonorum - Hortorum Cultus 14, 97–114.

Spears JW (2003) Trace mineral bioavailability in ruminants. The Journal of Nutrition 133, 1506S–1509S.
Trace mineral bioavailability in ruminants.Crossref | GoogleScholarGoogle Scholar | 12730454PubMed |

Spears JW, Kegley EB (2002) Effect of zinc source (zinc oxide vs zinc proteinate) and level on performance, carcass characteristics, and immune response of growing and finishing steers. Journal of Animal Science 80, 2747–2752.
Effect of zinc source (zinc oxide vs zinc proteinate) and level on performance, carcass characteristics, and immune response of growing and finishing steers.Crossref | GoogleScholarGoogle Scholar | 12413098PubMed |

Suttle NF (2010) ‘Mineral nutrition of livestock’, 4th edn. (CABI Publishing: Cambridge, UK)
| Crossref |

Tremblay GF, Bélanger G, Lajeunesse J, Chouinard PY, Charbonneau É (2015) Timothy response to increasing rates of selenium fertilizer in Eastern Canada. Agronomy Journal 107, 211–220.
Timothy response to increasing rates of selenium fertilizer in Eastern Canada.Crossref | GoogleScholarGoogle Scholar |

Wakeel A, Farooq M, Bashir K, Ozturk L (2018) Chapter 13 - Micronutrient malnutrition and biofortification: recent advances and future perspectives. In ‘Plant micronutrient use efficiency’. (Eds MA Hossain, T Kamiya, DJ Burritt, LSP Tran, T Fujiwara) pp. 225–243. (Elsevier: Amsterdam)
| Crossref |

Wang J, Wang Z, Mao H, Zhao H, Huang D (2013) Increasing Se concentration in maize grain with soil- or foliar-applied selenite on the Loess Plateau in China. Field Crops Research 150, 83–90.
Increasing Se concentration in maize grain with soil- or foliar-applied selenite on the Loess Plateau in China.Crossref | GoogleScholarGoogle Scholar |

Wang S, Li M, Liu K, Tian X, Li S, Chen Y, Jia Z (2017) Effects of Zn, macronutrients, and their interactions through foliar applications on winter wheat grain nutritional quality. PLoS ONE 12, e0181276
Effects of Zn, macronutrients, and their interactions through foliar applications on winter wheat grain nutritional quality.Crossref | GoogleScholarGoogle Scholar | 28746402PubMed |

Wang Y, Zhang X, Chen J, Chen A, Wang L, Guo X, Niu Y, Liu S, Mi G, Gao Q (2019) Reducing basal nitrogen rate to improve maize seedling growth, water and nitrogen use efficiencies under drought stress by optimizing root morphology and distribution. Agricultural Water Management 212, 328–337.
Reducing basal nitrogen rate to improve maize seedling growth, water and nitrogen use efficiencies under drought stress by optimizing root morphology and distribution.Crossref | GoogleScholarGoogle Scholar |

Wang S, Sun N, Yang S, Tian X, Liu Q (2021) The effectiveness of foliar applications of different zinc source and urea to increase grain zinc of wheat grown under reduced soil nitrogen supply. Journal of Plant Nutrition 44, 644–659.
The effectiveness of foliar applications of different zinc source and urea to increase grain zinc of wheat grown under reduced soil nitrogen supply.Crossref | GoogleScholarGoogle Scholar |

Wehrmann J, Scharpf HC (1979) Der mineralstickstoffgehalt des bodens als maßstab für den stickstoffdüngungsbedarf (Nmin-methode). Plant and Soil 52, 109–126.
Der mineralstickstoffgehalt des bodens als maßstab für den stickstoffdüngungsbedarf (Nmin-methode).Crossref | GoogleScholarGoogle Scholar |

White PJ, Broadley MR (2005) Biofortifying crops with essential mineral elements. Trends in Plant Science 10, 586–593.
Biofortifying crops with essential mineral elements.Crossref | GoogleScholarGoogle Scholar | 16271501PubMed |

Wyszkowski M, Brodowska MS (2021) Potassium and nitrogen fertilization vs. trace element content of maize (Zea mays L.). Agriculture 11, 96
Potassium and nitrogen fertilization vs. trace element content of maize (Zea mays L.).Crossref | GoogleScholarGoogle Scholar |

Xue Y-F, Yue S-C, Liu D-Y, Zhang W, Chen X-P, Zou C-Q (2019) Dynamic zinc accumulation and contributions of pre- and/or post-silking zinc uptake to grain zinc of maize as affected by nitrogen supply. Frontiers in Plant Science 10, 1203
Dynamic zinc accumulation and contributions of pre- and/or post-silking zinc uptake to grain zinc of maize as affected by nitrogen supply.Crossref | GoogleScholarGoogle Scholar | 31632429PubMed |

Zhang Y-Q, Sun Y-X, Ye Y-L, Karim M-R, Xue Y-F, Yan P, Meng Q-F, Cui ZL, Cakmak I, Zhang F-S, Zou C-Q (2012) Zinc biofortification of wheat through fertilizer applications in different locations of China. Field Crops Research 125, 1–7.
Zinc biofortification of wheat through fertilizer applications in different locations of China.Crossref | GoogleScholarGoogle Scholar |

Zhao F-J, McGrath SP (2009) Biofortification and phytoremediation. Current Opinion in Plant Biology 12, 373–380.
Biofortification and phytoremediation.Crossref | GoogleScholarGoogle Scholar | 19473871PubMed |