Agronomic assessment of the durum Rht18 dwarfing gene in bread wheat
G. J. Rebetzke
A * , A. R. Rattey B , W. D. Bovill A , R. A. Richards A , B. J. Brooks A and M. Ellis C
+ Author Affiliations
- Author Affiliations
A CSIRO Agriculture and Food, PO Box 1700, Canberra, ACT 2601 Australia.
B Formerly CSIRO now Intergrain, 19 Ambitious Link, Bibra Lake WA 6162 Australia.
C Formerly CSIRO now 8 Avenue Piaton, Villeurbanne, France.
Crop & Pasture Science 73(4) 325-336 https://doi.org/10.1071/CP21645
Submitted: 29 August 2021 Accepted: 3 November 2021 Published: 14 February 2022
© 2022 The Author(s) (or their employer(s)). Published by CSIRO Publishing. This is an open access article distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND)
Abstract
The wheat Green Revolution Rht-B1b and Rht-D1b dwarfing alleles are associated with increased grain yields but also with reduced early growth and seedling emergence, especially if sowing conditions are unfavourable. The gibberellic acid-responsive, mutagen-derived Rht18 dwarfing gene was backcrossed from durum wheat (Triticum turgidum subsp. durum L.) cv. Icaro into tall bread wheat (Triticum aestivum L.) cv. Halberd using phenotypic selection for reduced plant height. The Rht18 allele was confirmed among homozygous BC1F2-derived, F5:7 recombinant inbred lines by using a chromosome 6AS-linked, microsatellite molecular marker (Xwms4608), and then assessed for agronomic performance across multiple field sites ranging in yield from 3.6 to 6.4 t/ha. The Rht18-containing lines were significantly (P < 0.05) shorter in height (−24%) and reduced in plant lodging (−51%) compared with tall sister lines. Reductions in plant height were associated with significant increases in grain yield (+16%), reflecting increases in grain number (+21%), number of spikes (+7%) and number of grains per spike (+12%). Coleoptile length, early shoot biomass and ground cover percentage were unaffected by the presence of the Rht18 dwarfing gene. Comparisons of effects of gibberellic acid-insensitive Rht-B1b and Rht18 on early growth and agronomic performance were assessed separately for a set of 30 BC5F6-derived Halberd near-isogenic lines in the field in 2015. Ground cover and coleoptile length were significantly greater for Rht18 lines, whereas plant height, lodging, harvest index, grain number and yield were similar for Rht-B1b and Rht18 sister lines. Reduced lodging and increased grain number and yield, together with greater coleoptile length, indicate a potentially useful role for Rht18 in improving wheat performance.
Keywords: coleoptile, dwarf, early vigour, establishment, germplasm, harvest index, lodging, physiology.
References
Addisu M, Snape JW, Simmonds JR, Gooding MJ (2009) Reduced height (Rht) and photoperiod insensitivity (Ppd) allele associations with establishment and early growth of wheat in contrasting production systems. Euphytica 166, 249–267.| Reduced height (Rht) and photoperiod insensitivity (Ppd) allele associations with establishment and early growth of wheat in contrasting production systems.Crossref | GoogleScholarGoogle Scholar |
Allan RE (1989) Agronomic comparisons between Rht1 and Rht2 semidwarf genes in winter wheat. Crop Science 29, 1103–1108.
| Agronomic comparisons between Rht1 and Rht2 semidwarf genes in winter wheat.Crossref | GoogleScholarGoogle Scholar |
Botwright TL, Rebetzke GJ, Condon AG, Richards RA (2005) Influence of the gibberellin-sensitive Rht8 dwarfing gene on leaf epidermal cell dimensions and early vigour in wheat (Triticum aestivum L.). Annals of Botany 95, 631–639.
| Influence of the gibberellin-sensitive Rht8 dwarfing gene on leaf epidermal cell dimensions and early vigour in wheat (Triticum aestivum L.).Crossref | GoogleScholarGoogle Scholar | 15655105PubMed |
Brandle JE, Knott DR (1986) The effect of a gene for semi-dwarfism (Rht1) on various characters in a spring wheat cross. Canadian Journal of Plant Science 66, 529–533.
| The effect of a gene for semi-dwarfism (Rht1) on various characters in a spring wheat cross.Crossref | GoogleScholarGoogle Scholar |
Butler JD, Byrne PF, Mohammadi V, Chapman PL, Haley SD (2005) Agronomic performance of Rht alleles in a spring wheat population across a range of moisture levels. Crop Science 45, 939–947.
| Agronomic performance of Rht alleles in a spring wheat population across a range of moisture levels.Crossref | GoogleScholarGoogle Scholar |
Chen L, Phillips AL, Condon AG, Parry MAJ, Hu Y-G (2013) GA-responsive dwarfing gene Rht12 affects the developmental and agronomic traits in common bread wheat. PloS ONE 8, e62285
| GA-responsive dwarfing gene Rht12 affects the developmental and agronomic traits in common bread wheat.Crossref | GoogleScholarGoogle Scholar | 23658622PubMed |
Coleman RK, Gill GS, Rebetzke GJ (2001) Identification of quantitative trait loci (QTL) for traits conferring weed competitiveness in wheat (Triticum aestivum L.). Australian Journal of Agricultural Research 52, 1235–1246.
Cullis BR, Thomson FM, Fisher JA, Gilmour AR, Thompson R (1996) The analysis of the NSW wheat variety database. II. Variance component estimation. Theoretical and Applied Genetics 92, 28–39.
| The analysis of the NSW wheat variety database. II. Variance component estimation.Crossref | GoogleScholarGoogle Scholar | 24166113PubMed |
Daoura BG, Chen L, Du Y, Hu Y-G (2014) Genetic effects of dwarfing gene Rht5 on agronomic traits in common wheat (Triticum aestivum L.) and QTL analysis on its linked traits. Field Crops Research 156, 22–29.
| Genetic effects of dwarfing gene Rht5 on agronomic traits in common wheat (Triticum aestivum L.) and QTL analysis on its linked traits.Crossref | GoogleScholarGoogle Scholar |
Ellis M, Spielmeyer W, Gale K, Rebetzke G, Richards R (2002) ‘Perfect‘ markers for the Rht-B1b and Rht-D1b dwarfing mutations in wheat (Triticum aestivum L.). Theoretical and Applied Genetics 105, 1038–1042.
| ‘Perfect‘ markers for the Rht-B1b and Rht-D1b dwarfing mutations in wheat (Triticum aestivum L.).Crossref | GoogleScholarGoogle Scholar |
Ellis MH, Rebetzke GJ, Chandler P, Bonnett D, Spielmeyer W, Richards RA (2004) The effect of different height reducing genes on the early growth of wheat. Functional Plant Biology 31, 583–589.
| The effect of different height reducing genes on the early growth of wheat.Crossref | GoogleScholarGoogle Scholar | 32688930PubMed |
Evans LT (1998) Feeding the ten billion: plants and population growth. Cambridge University Press.
Fischer RA, Stockman YM (1986) Increased kernel number in Norin 10-derived dwarf wheat: evaluation of the cause. Australian Journal of Plant Physiology 13, 767–784.
| Increased kernel number in Norin 10-derived dwarf wheat: evaluation of the cause.Crossref | GoogleScholarGoogle Scholar |
Flintham JE, Borner A, Worland AJ, Gale MD (1997) Optimizing wheat grain yield: effects of Rht (gibberellin-insenstive) dwarfing genes. The Journal of Agricultural Science (Camb.) 128, 11–25.
| Optimizing wheat grain yield: effects of Rht (gibberellin-insenstive) dwarfing genes.Crossref | GoogleScholarGoogle Scholar |
Flohr BM, Ouzman J, McBeath TM, Rebetzke GJ, Kirkegaard JA, Llewellyn RS (2021) Spatial analysis of the seasonal break and implications for crop establishment in southern Australia. Agricultural Systems 190, 103105
Ford BA, Foo E, Sharwood R, Karafiatova M, Vrána J, MacMillan C, Nichols DS, Steuernagel B, Uauy C, Doležel J, Chandler PM, Spielmeyer W (2018) Rht18 semidwarfism in wheat is due to increased GA 2-oxidaseA9 expression and reduced GA content. Plant Physiology 177, 168–180.
| Rht18 semidwarfism in wheat is due to increased GA 2-oxidaseA9 expression and reduced GA content.Crossref | GoogleScholarGoogle Scholar | 29545269PubMed |
Haque M, Martinek P, Watanabe N, Kuboyama T (2011) Genetic mapping of gibberellic acid-sensitive genes for semi-dwarfism in durum wheat. Cereal Research Communications 39, 171–178.
| Genetic mapping of gibberellic acid-sensitive genes for semi-dwarfism in durum wheat.Crossref | GoogleScholarGoogle Scholar |
Hedden P (2003) The genes of the green revolution. Trends in Genetics 19, 5–9.
| The genes of the green revolution.Crossref | GoogleScholarGoogle Scholar | 12493241PubMed |
Holland JB (2006) Estimating genotypic correlations and their standard errors using multivariate restricted maximum likelihood estimation with SAS Proc MIXED. Crop Science 46, 642–654.
| Estimating genotypic correlations and their standard errors using multivariate restricted maximum likelihood estimation with SAS Proc MIXED.Crossref | GoogleScholarGoogle Scholar |
Hoogendoorn J, Rickson JM, Gale MD (1990) Differences in leaf and stem anatomy related to plant height of tall and dwarf wheat (Triticum aestivum L.). Journal of Plant Physiology 136, 72–77.
| Differences in leaf and stem anatomy related to plant height of tall and dwarf wheat (Triticum aestivum L.).Crossref | GoogleScholarGoogle Scholar |
Keyes GJ, Paolillo DJ, Sorrells ME (1989) The effects of dwarfing genes Rht1 and Rht2 on cellular dimensions and rate of leaf elongation in wheat. Annals of Botany 64, 683–690.
| The effects of dwarfing genes Rht1 and Rht2 on cellular dimensions and rate of leaf elongation in wheat.Crossref | GoogleScholarGoogle Scholar |
Konzak CF (1988) Genetic analysis, genetic improvement and evaluation of induced semi-dwarf mutants in wheat. In ‘Semi-dwarf cereal mutants and their use in cross-breeding III. Research coordination meeting, 16–20 December 1985’. pp. 76–94. (International Atomic Energy Agency: Vienna, Austria)
Li Y, Chen D, Walker CN, Angus JF (2010) Estimating the nitrogen status of crops using a digital camera. Field Crops Research 118, 221–227.
| Estimating the nitrogen status of crops using a digital camera.Crossref | GoogleScholarGoogle Scholar |
Loskutova NP (1998) The influence of Rht 1–5, Rht 8–9 and Rht 13 genes on morphological characters and yield productivity of wheat. In ‘Proceedings of the 9th International wheat genetics symposium’. (Ed. AE Slinkard) pp. 283–284. (University Extension Press, University of Saskatchewan: Saskatoon, SK, Canada)
Mathews KL, Chapman SC, Trethowan R, Singh RP, Crossa J, Pfeiffer W, van Ginkel M, DeLacy I (2006) Global adaptation of spring bread and durum wheat lines near-isogenic for major reduced height genes. Crop Science 46, 603–613.
| Global adaptation of spring bread and durum wheat lines near-isogenic for major reduced height genes.Crossref | GoogleScholarGoogle Scholar |
McCartney CA, Somers DJ, Lukow O, Ames N, Noll J, Cloutier S, Humphreys DG, McCallum BD (2006) QTL analysis of quality traits in the spring wheat cross RL4452 × ‘AC Domain’. Plant Breeding 125, 565–575.
| QTL analysis of quality traits in the spring wheat cross RL4452 × ‘AC Domain’.Crossref | GoogleScholarGoogle Scholar |
McIntosh MS (1983) Analysis of combined experiments. Agronomy Journal 75, 153–155.
| Analysis of combined experiments.Crossref | GoogleScholarGoogle Scholar |
McIntosh RA, Hart GE, Devos KM, Gale MD, Rogers WJ (1998) Catalogue of gene symbols for wheat. In: ‘Proceedings 9th International Wheat Genetics Symposium, Vol. 5’. Saskatoon: Canada. pp. 1–235
Pang J, Palta JA, Rebetzke GJ, Milroy SP (2014) Wheat genotypes with high early vigour accumulate more nitrogen and have higher photosynthetic nitrogen use efficiency during early growth. Functional Plant Biology 41, 215–222.
| Wheat genotypes with high early vigour accumulate more nitrogen and have higher photosynthetic nitrogen use efficiency during early growth.Crossref | GoogleScholarGoogle Scholar | 32480980PubMed |
Perry MW, D’Antuono MF (1989) Yield improvement and associated characteristics of some Australian spring wheat cultivars introduced between 1860 and 1982. Australian Journal of Agricultural Research 40, 457–472.
| Yield improvement and associated characteristics of some Australian spring wheat cultivars introduced between 1860 and 1982.Crossref | GoogleScholarGoogle Scholar |
Rebetzke GJ, Richards RA (1999) Genetic improvement of early vigour in wheat. Australian Journal of Agricultural Research 50, 291–301.
| Genetic improvement of early vigour in wheat.Crossref | GoogleScholarGoogle Scholar |
Rebetzke GJ, Richards RA (2000) Gibberellic acid-sensitive dwarfing genes reduce plant height to increase kernel number and grain yield of wheat. Australian Journal of Agricultural Research 51, 235–245.
| Gibberellic acid-sensitive dwarfing genes reduce plant height to increase kernel number and grain yield of wheat.Crossref | GoogleScholarGoogle Scholar |
Rebetzke GJ, Appels R, Morrison A, Richards RA, McDonald G, Ellis MH, Spielmeyer W, Bonnett DG (2001) Quantitative trait loci on chromosome 4B for coleoptile length and early vigour in wheat (Triticum aestivum L.). Australian Journal of Agricultural Research 52, 1221–1234.
Rebetzke GJ, Ellis MH, Bonnett DG, Richards RA (2007) Molecular mapping of genes for coleoptile growth in bread wheat (Triticum aestivum L.). Theoretical and Applied Genetics 114, 1173–1183.
| Molecular mapping of genes for coleoptile growth in bread wheat (Triticum aestivum L.).Crossref | GoogleScholarGoogle Scholar | 17294164PubMed |
Rebetzke GJ, Ellis MH, Bonnett DG, Condon AG, Falk D, Richards RA (2011) The Rht13 dwarfing gene reduces peduncle length and plant height to increase grain number and yield of wheat. Field Crops Research 124, 323–331.
| The Rht13 dwarfing gene reduces peduncle length and plant height to increase grain number and yield of wheat.Crossref | GoogleScholarGoogle Scholar |
Rebetzke GJ, Ellis MH, Bonnett DG, Mickelson B, Condon AG, Richards RA (2012) Height reduction and agronomic performance for selected gibberellin-responsive dwarfing genes in bread wheat (Triticum aestivum L.). Field Crops Research 126, 87–96.
| Height reduction and agronomic performance for selected gibberellin-responsive dwarfing genes in bread wheat (Triticum aestivum L.).Crossref | GoogleScholarGoogle Scholar |
Rebetzke GJ, Chenu K, Biddulph B, Moeller C, Deery DM, Rattey AR, Bennett D, Barrett-Lennard EG, Mayer JE (2013) A multisite managed environment facility for targeted trait and germplasm phenotyping. Functional Plant Biology 40, 1–13.
| A multisite managed environment facility for targeted trait and germplasm phenotyping.Crossref | GoogleScholarGoogle Scholar |
Rebetzke GJ, Verbyla AP, Verbyla KL, Morell MK, Cavanagh CR (2014) Use of a large multiparent wheat mapping population in genomic dissection of coleoptile and seedling growth. Plant Biotechnology Journal 12, 219–230.
| Use of a large multiparent wheat mapping population in genomic dissection of coleoptile and seedling growth.Crossref | GoogleScholarGoogle Scholar | 24151921PubMed |
Richards RA (1992) The effect of dwarfing genes in spring wheat in dry environments. I. Agronomic characteristics. Australian Journal of Agricultural Research 43, 517–527.
| The effect of dwarfing genes in spring wheat in dry environments. I. Agronomic characteristics.Crossref | GoogleScholarGoogle Scholar |
Ryan PR, Liao M, Delhaize E, Rebetzke GJ, Weligama K, Spielmeyer W, James R (2015) Early vigour and phosphate uptake in bread wheat. Journal of Experimental Botany 66, 7089–7100.
Singh RP, Huerta-Espino J, Rajaram S, Crossa J (2001) Grain yield and other traits of tall and dwarf isolines of modern bread and durum wheats. Euphytica 119, 241–244.
| Grain yield and other traits of tall and dwarf isolines of modern bread and durum wheats.Crossref | GoogleScholarGoogle Scholar |
Tang T (2016) Physiological and genetic studies of an alternative semi-dwarfing gene Rht18 in wheat. PhD Thesis, University of Tasmania, Hobart, Tas., Australia.
Yang Z, Zheng J, Liu C, Wang Y, Condon AG, Chen Y, Hu Y-G (2015) Effects of the GA-responsive dwarfing gene Rht18 from tetraploid wheat on agronomic traits of common wheat. Field Crops Research 183, 92–101.
| Effects of the GA-responsive dwarfing gene Rht18 from tetraploid wheat on agronomic traits of common wheat.Crossref | GoogleScholarGoogle Scholar |
Zadoks JC, Chang TT, Konzak CF (1974) A decimal code for the growth stages of cereals. Weed Research 14, 415–421.
| A decimal code for the growth stages of cereals.Crossref | GoogleScholarGoogle Scholar |
Zerner RK, Gill GS, Rebetzke GJ (2016) Stability of wheat cultivars in weed competitive ability in differing environments in southern Australia. Crop and Pasture Science 67, 695–702.
