Salinity tolerance and avoidance mechanisms at germination among messina (Melilotus siculus) accessions
Robert P. Jeffery
A C , Megan H. Ryan A , Natasha L. Ayers A B and Phillip G. H. Nichols A
+ Author Affiliations
- Author Affiliations
A School of Agriculture and Environment and Institute of Agriculture, University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.
B Current address: AgriStart, 1/14 Burler Drive, Vasse, WA 6280, Australia.
C Corresponding author. Email: robert.peter.jeffery@gmail.com
Crop and Pasture Science - https://doi.org/10.1071/CP20427
Submitted: 10 November 2020 Accepted: 17 February 2021 Published online: 21 June 2021
Journal Compilation © CSIRO 2021 Open Access CC BY
Abstract
Messina (Melilotus siculus) cv. Neptune, an annual pasture legume native to the Mediterranean Basin, has recently been released for saltland pastures in southern Australia following demonstration of biomass production and persistence superior to other commercial pasture legumes in saline environments prone to winter waterlogging. Self-regenerating annual pasture legumes also require seed adaptations for both tolerating and avoiding salinity at germination in these environments. This study examined diversity within Neptune and 20 other messina accessions for salt tolerance at germination, recovery of germinability from temporary salt stress, and timing and extent of hardseed softening, compared with balansa clover (Trifolium michelianum) cv. Frontier, burr medic (Medicago polymorpha) cv. Scimitar and white melilot (Melilotus albus) cv. Jota. Germination rates after 14 days at 300 mM NaCl relative to 0 mM NaCl were ≥99% for Neptune and 18 other messina accessions, 66% for Scimitar, 21% for Jota and 11% for Frontier. No genotype germinated at 600 mM NaCl; however, when transferred to 0 mM NaCl after 14 days at 600 mM, all genotypes except Scimitar and Jota recovered partial germination, ranging from 13% to 93% of controls (0 mM NaCl for 28 days). The softening rate of hard (impermeable) seeds in the field varied among genotypes, with deferral of hardseed softening until late autumn–early winter, when rainfall is more likely, indicating greater persistence. The months in which the hardseed level first became significantly lower than the initial level (in freshly harvested seeds) after placement on the soil surface in December were: March for Frontier; April for Scimitar, Jota and Neptune; and March–July for all messina accessions. This study confirmed that messina has high salt tolerance and several avoidance mechanisms at germination that contribute to its adaptation to saline soils in southern Australia. Several messina accessions were superior to Neptune for individual traits which could be exploited for plant breeding. These results also have implications for saltland pastures in other regions of the world with Mediterranean-type climates.
Keywords: genetic resources, germination, hardseededness, Melilotus, pasture legumes, salinity tolerance.
References
Ballard RA, Charman N, Bonython AL, Nichols PGH, Craig AD (2014) SRDI 544: a new inoculant strain of Sinorhizobium medicae for use on messina (Melilotus siculus): an annual legume for salt-land pastures. In ‘Proceedings of the 17th Australian Nitrogen Fixation Conference’. Adelaide, S. Aust. (Eds VVSR Gupta, M Unkovich, BN Kaiser) pp. 92–94. (Australian Society of Nitrogen Fixation: Adelaide, SA)Barrett-Lennard EG, Shabala SN (2013) The waterlogging/salinity interaction in higher plants revisited: focusing on the hypoxia-induced disturbance to K+ homeostasis. Functional Plant Biology 40, 872–882.
| The waterlogging/salinity interaction in higher plants revisited: focusing on the hypoxia-induced disturbance to K+ homeostasis.Crossref | GoogleScholarGoogle Scholar | 32481157PubMed |
Barrett-Lennard EG, Bennett SJ, Altman M (2013) Survival and growth of perennial halophytes on saltland in a Mediterranean environment is affected by depth to watertable in summer as well as subsoil salinity. Crop & Pasture Science 64, 123–136.
| Survival and growth of perennial halophytes on saltland in a Mediterranean environment is affected by depth to watertable in summer as well as subsoil salinity.Crossref | GoogleScholarGoogle Scholar |
Bennett SJ, Barrett-Lennard EG, Colmer TD (2009) Salinity and waterlogging as constraints to saltland pasture production: a review. Agriculture, Ecosystems & Environment 129, 349–360.
| Salinity and waterlogging as constraints to saltland pasture production: a review.Crossref | GoogleScholarGoogle Scholar |
Bonython AL, Ballard RA, Charman N, Nichols PGH, Craig AD (2011) New strains of rhizobia that nodulate regenerating messina (Melilotus siculus) plants in saline soils. Crop & Pasture Science 62, 427–436.
| New strains of rhizobia that nodulate regenerating messina (Melilotus siculus) plants in saline soils.Crossref | GoogleScholarGoogle Scholar |
Chapman R, Asseng S (2001) An analysis of the frequency and timing of false break events in the Mediterranean region of Western Australia. Australian Journal of Agricultural Research 52, 367–376.
| An analysis of the frequency and timing of false break events in the Mediterranean region of Western Australia.Crossref | GoogleScholarGoogle Scholar |
Dear BS, Reed KFM, Craig AD (2008) Outcomes of the search for new perennial and salt tolerant pasture plants for southern Australia. Animal Production Science 48, 578–588.
| Outcomes of the search for new perennial and salt tolerant pasture plants for southern Australia.Crossref | GoogleScholarGoogle Scholar |
DPIRD (2019) Neptune messina: a new pasture legume for saline soils prone to waterlogging. Agriculture and Food, Department of Primary Industries and Regional Development, Perth, W. Aust. Available at: https://www.agric.wa.gov.au/neptune (accessed 10 August 2020)
Evans PM, Kearney GA (2003) Melilotus albus (Medik.) is productive and regenerates well on saline soils of neutral to alkaline reaction in the high rainfall zone of south-western Victoria. Australian Journal of Experimental Agriculture 43, 349–355.
| Melilotus albus (Medik.) is productive and regenerates well on saline soils of neutral to alkaline reaction in the high rainfall zone of south-western Victoria.Crossref | GoogleScholarGoogle Scholar |
Government of Western Australia (2000) Natural resource management in Western Australia: State Salinity Strategy 2000. Prepared by the State Salinity Council, Perth, W. Aust.
Guan B, Zhou D, Zhang H, Tian Y, Japhet W, Wang P (2009) Germination responses of Medicago ruthenica seeds to salinity, alkalinity, and temperature. Journal of Arid Environments 73, 135–138.
| Germination responses of Medicago ruthenica seeds to salinity, alkalinity, and temperature.Crossref | GoogleScholarGoogle Scholar |
Howieson JG, O’Hara GW, Carr SJ (2000) Changing roles for legumes in Mediterranean agriculture: developments from an Australian perspective. Field Crops Research 65, 107–122.
| Changing roles for legumes in Mediterranean agriculture: developments from an Australian perspective.Crossref | GoogleScholarGoogle Scholar |
Hughes SJ, Snowball R, Reed KFM, Cohen B, Gajda K, Williams AR, Groeneweg SL (2008) The systematic collection and characterisation of herbaceous forage species for recharge and discharge environments in southern Australia. Australian Journal of Experimental Agriculture 48, 397–408.
| The systematic collection and characterisation of herbaceous forage species for recharge and discharge environments in southern Australia.Crossref | GoogleScholarGoogle Scholar |
Kotula L, Kwa HY, Nichols PGH, Colmer TD (2019) Tolerance and recovery of the annual pasture legumes Melilotus siculus, Trifolium michelianum and Medicago polymorpha to soil salinity, soil waterlogging and the combination of these stresses. Plant and Soil 444, 267–280.
| Tolerance and recovery of the annual pasture legumes Melilotus siculus, Trifolium michelianum and Medicago polymorpha to soil salinity, soil waterlogging and the combination of these stresses.Crossref | GoogleScholarGoogle Scholar |
Loi A, Howieson JG, Nutt BJ, Carr SJ (2005) A second generation of annual pasture legumes and their potential for inclusion in Mediterranean-type farming systems. Australian Journal of Experimental Agriculture 45, 289–299.
| A second generation of annual pasture legumes and their potential for inclusion in Mediterranean-type farming systems.Crossref | GoogleScholarGoogle Scholar |
Marañon T, Garcia LV, Troncoso A (1989) Salinity and germination of annual Melilotus from the Guadalquivir Delta (SW Spain). Plant and Soil 119, 223–228.
| Salinity and germination of annual Melilotus from the Guadalquivir Delta (SW Spain).Crossref | GoogleScholarGoogle Scholar |
McFarlane DJ, Williamson DR (2002) An overview of water logging and salinity in southwestern Australia as related to the ‘Ucarro’ experimental catchment. Agricultural Water Management 53, 5–29.
| An overview of water logging and salinity in southwestern Australia as related to the ‘Ucarro’ experimental catchment.Crossref | GoogleScholarGoogle Scholar |
Nichols PGH, Loi A, Nutt BJ, Evans PM, Craig AD, Pengelly BC, Dear BS, Lloyd DL, Revell CK, Nair RM, Ewing MA, Howieson JG, Auricht GA, Howie JH, Sandral GA, Carr SJ, de Koning CT, Hackney BF, Crocker GJ, Snowball R, Hughes SJ, Hall EJ, Foster KJ, Skinner PW, Barbetti MJ, You MP (2007) New annual and short-lived perennial pasture legumes for Australian agriculture: 15 years of revolution. Field Crops Research 104, 10–23.
| New annual and short-lived perennial pasture legumes for Australian agriculture: 15 years of revolution.Crossref | GoogleScholarGoogle Scholar |
Nichols PGH, Craig AD, Rogers ME, Albertsen TO, Miller SM, McClements DR, Hughes SJ, D’Antuono MF, Dear BS (2008) Production and persistence of annual pasture legumes at five saline sites in southern Australia. Australian Journal of Experimental Agriculture 48, 518–535.
| Production and persistence of annual pasture legumes at five saline sites in southern Australia.Crossref | GoogleScholarGoogle Scholar |
Nichols PGH, Malik AI, Stockdale M, Colmer TD (2009) Salt tolerance and avoidance mechanisms at germination of annual pasture legumes: importance for adaptation to saline environments. Plant and Soil 315, 241–255.
| Salt tolerance and avoidance mechanisms at germination of annual pasture legumes: importance for adaptation to saline environments.Crossref | GoogleScholarGoogle Scholar |
Nichols PGH, Barrett-Lennard EG, Bennett S (2010) Pasture legumes and grasses for saltland. Farmnote 432, June 2010. Department of Agriculture and Food, Western Australia, Perth.
Nichols PGH, Revell CK, Humphries AW, Howie JH, Hall EJ, Sandral GA, Ghamkhar K, Harris CA (2012) Temperate pasture legumes in Australia—their history, current use, and future prospects. Crop & Pasture Science 63, 691–725.
| Temperate pasture legumes in Australia—their history, current use, and future prospects.Crossref | GoogleScholarGoogle Scholar |
Nichols PGH, Ballard RA, Pearce AL, Wintle BJ, Craig AD (2019) ‘Neptune’, the world’s first messina (Melilotus siculus) cultivar: an annual pasture legume for saline soils prone to winter waterlogging. In ‘Cells to Satellites. Proceedings of the 19th Agronomy Australia Conference’. 25–29 August 2019. Wagga Wagga, NSW. (Ed. J Pratley) http://www.agronomyaustraliaproceedings.org/
NLWRA (2001) Australian dryland salinity assessment 2000. Extent, impacts, processes, monitoring and management options. National Land and Water Resources Audit, Canberra, ACT.
Norman HC, Cocks PS, Smith FP, Nutt BJ (1998) Reproductive strategies in Mediterranean annual clovers: germination and hardseededness. Australian Journal of Agricultural Research 49, 973–982.
| Reproductive strategies in Mediterranean annual clovers: germination and hardseededness.Crossref | GoogleScholarGoogle Scholar |
Norman HC, Cocks PS, Galwey NW (2005) Annual clovers (Trifolium spp.) have different reproductive strategies to achieve persistence in Mediterranean-type climates. Australian Journal of Agricultural Research 56, 33–43.
| Annual clovers (Trifolium spp.) have different reproductive strategies to achieve persistence in Mediterranean-type climates.Crossref | GoogleScholarGoogle Scholar |
Norman HC, Smith FP, Nichols PGH, Si P, Galwey NW (2006) Variation in seed softening patterns and impact of seed production environment on hardseededness in early maturing genotypes of subterranean clover. Australian Journal of Agricultural Research 57, 65–74.
| Variation in seed softening patterns and impact of seed production environment on hardseededness in early maturing genotypes of subterranean clover.Crossref | GoogleScholarGoogle Scholar |
Porqueddu C, Ates S, Louhaichi M, Kyriazopoulos AP, Moreno G, del Pozo A, Ovalle C, Ewing MA, Nichols PGH (2016) Grasslands in ‘Old World’ and ‘New World’ Mediterranean-climate zones: past trends, current status and future research priorities. Grass and Forage Science 71, 1–35.
| Grasslands in ‘Old World’ and ‘New World’ Mediterranean-climate zones: past trends, current status and future research priorities.Crossref | GoogleScholarGoogle Scholar |
Redmann RE (1974) Osmotic and specific ion effects on the germination of alfalfa. Canadian Journal of Botany 52, 803–808.
| Osmotic and specific ion effects on the germination of alfalfa.Crossref | GoogleScholarGoogle Scholar |
Robson AD (1990) The role of self-regenerating pasture in rotation with cereals in Mediterranean areas. In ‘The role of legumes in the farming systems of the Mediterranean areas’. Developments in Plant and Soil Sciences Vol. 38. (Eds AE Osman, MH Ibrahim, MA Jones) pp. 217–236. (Springer)
Rogers ME, Noble CL (1991) The effect of NaCl on the establishment and growth of balansa clover (Trifolium michelianium Savi. var. balansae Boiss.). Australian Journal of Agricultural Research 42, 847–857.
| The effect of NaCl on the establishment and growth of balansa clover (Trifolium michelianium Savi. var. balansae Boiss.).Crossref | GoogleScholarGoogle Scholar |
Rogers ME, Craig AD, Munns RE, Colmer TD, Nichols PGH, Malcolm CV, Barrett-Lennard E, Brown AJ, Semple WS, Evans PM, Cowley K, Hughes SJ, Snowball R, Bennett SJ, Sweeney GC, Dear BS, Ewing MA (2005) The potential for developing fodder plants for the salt-affected areas of southern and eastern Australia: an overview. Australian Journal of Experimental Agriculture 45, 301–329.
| The potential for developing fodder plants for the salt-affected areas of southern and eastern Australia: an overview.Crossref | GoogleScholarGoogle Scholar |
Rogers ME, Colmer TD, Frost K, Henry D, Cornwall D, Hulm E, Deretic J, Hughes SR, Craig AD (2008) Diversity in the genus Melilotus for tolerance to salinity and waterlogging. Plant and Soil 304, 89–101.
| Diversity in the genus Melilotus for tolerance to salinity and waterlogging.Crossref | GoogleScholarGoogle Scholar |
Rogers ME, Colmer TD, Nichols PGH, Hughes SJ, Frost K, Cornwall D, Chandra S, Miller SM, Craig AD (2011) Salinity and waterlogging tolerance amongst accessions of messina (Melilotus siculus). Crop & Pasture Science 62, 225–235.
| Salinity and waterlogging tolerance amongst accessions of messina (Melilotus siculus).Crossref | GoogleScholarGoogle Scholar |
Smetham ML (2003) A review of subterranean clover (Trifolium subterraneum L.): its ecology, and use as a pasture legume in Australasia. Advances in Agronomy 79, 303–350.
| A review of subterranean clover (Trifolium subterraneum L.): its ecology, and use as a pasture legume in Australasia.Crossref | GoogleScholarGoogle Scholar |
Striker GG, Colmer TD (2016) Flooding tolerance of forage legumes. Journal of Experimental Botany 68, 1851–1872.
| Flooding tolerance of forage legumes.Crossref | GoogleScholarGoogle Scholar |
Striker GG, Teakle NL, Colmer TD, Barrett-Lennard EG (2015) Growth responses of Melilotus siculus accessions to combined salinity and root-zone hypoxia are correlated with differences in tissue ion concentrations and not differences in root aeration. Environmental and Experimental Botany 109, 89–98.
| Growth responses of Melilotus siculus accessions to combined salinity and root-zone hypoxia are correlated with differences in tissue ion concentrations and not differences in root aeration.Crossref | GoogleScholarGoogle Scholar |
Taylor GB (2005) Hardseededness in Mediterranean annual pasture legumes in Australia: a review. Australian Journal of Agricultural Research 56, 645–661.
| Hardseededness in Mediterranean annual pasture legumes in Australia: a review.Crossref | GoogleScholarGoogle Scholar |
Teakle NL, Snell A, Real D, Barrett-Lennard EG, Colmer TD (2010) Variation in salinity tolerance, early shoot mass and shoot ion concentrations within Lotus tenuis: towards a perennial pasture legume for saline land. Crop & Pasture Science 61, 379–388.
| Variation in salinity tolerance, early shoot mass and shoot ion concentrations within Lotus tenuis: towards a perennial pasture legume for saline land.Crossref | GoogleScholarGoogle Scholar |
Teakle NL, Armstrong J, Barrett-Lennard EG, Colmer TD (2011) Aerenchymatous phellem in hypocotyl and roots enables O2 transport in Melilotus siculus. New Phytologist 190, 340–350.
| Aerenchymatous phellem in hypocotyl and roots enables O2 transport in Melilotus siculus.Crossref | GoogleScholarGoogle Scholar |
Teakle NL, Bowman S, Barrett-Lennard EG, Real D, Colmer TD (2012) Comparisons of annual pasture legumes in growth, ion regulation and root porosity demonstrate that Melilotus siculus has exceptional tolerance to combinations of salinity and waterlogging. Environmental and Experimental Botany 77, 175–184.
| Comparisons of annual pasture legumes in growth, ion regulation and root porosity demonstrate that Melilotus siculus has exceptional tolerance to combinations of salinity and waterlogging.Crossref | GoogleScholarGoogle Scholar |
Ungar IA (1962) Influence of salinity on seed germination in succulent halophytes. Ecology 43, 763–764.
| Influence of salinity on seed germination in succulent halophytes.Crossref | GoogleScholarGoogle Scholar |
West DW, Taylor JA (1981) Germination and growth of cultivars of Trifolium subterraneum L. in the presence of sodium chloride salinity. Plant and Soil 62, 221–230.
| Germination and growth of cultivars of Trifolium subterraneum L. in the presence of sodium chloride salinity.Crossref | GoogleScholarGoogle Scholar |
