Genetic dissection of 2-heptenal content in soybean (Glycine max) seed through genome-wide association study
Zhikun Wang A * , Mingming Yang A * , Yuanzhuo Wang A * , Chao Yang A , Xue Zhao
A B , Lizheng Tian A , Yupeng Shao A , Gege Bao A , Jinxiu Huang A , Ning Xia A B and Yingpeng Han A B
+ Author Affiliations
- Author Affiliations
A Key Laboratory of Soybean Biology in Chinese Ministry of Education (Northeastern Key Laboratory of Soybean Biology and Genetics and Breeding in Chinese Ministry of Agriculture), Northeast Agricultural University, Harbin 150030, China.
B Corresponding authors. Email: hyp234286@aliyun.com; xianing1981@126.com; 191182112@qq.com
Crop and Pasture Science 71(10) 884-893 https://doi.org/10.1071/CP20237
Submitted: 6 July 2020 Accepted: 8 September 2020 Published: 29 October 2020
Abstract
Association analysis is an alternative to conventional, family-based methods for detecting the location of gene(s) or quantitative trait loci (QTLs), and provides relatively high resolution in terms of defining the genome position of a gene or QTL. Flavour is an essential quality characteristic of soymilk; however, soymilk contains volatile compounds unacceptable to consumers. One of main constituents in the volatiles of normal soymilk is 2-heptenal, which is thought to be a degradative oxidation product of polyunsaturated acids. In this study, a genome-wide association study using 24 651 single-nucleotide polymorphisms (SNPs) was performed to identify quantitative trait nucleotides (QTNs) controlling 2-heptenal content in soybean (Glycine max (L.) Merr.) seed from a natural population of 110 soybean germplasm accessions. We detected 62 significant QTNs located on 18 different chromosomes that are significantly associated with 2-heptenal content in soybean seed. Among these, 17 QTNs co-localised with QTLs previously found to be related to protein, oil and/or fatty acid content in soybean seed. We also identified some candidate genes involved in lipid metabolism. These findings further our understanding of the genetic basis of 2-heptenal content in soybean seed and the improvement of marker-assisted breeding efficiency, which will be important for breeding soybean cultivars with low 2-heptenal content.
Keywords: candidate genes, GWAS, 2-heptenal concentration, quantitative trait nucleotides, SNP, soybean.
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