Identification of candidate genes for lutein esterification in common wheat (Triticum aestivum) using physical mapping and genomics tools
C. M. Ávila A , M. C. Palomino B , D. Hornero-Méndez C and S. G. Atienza
B D
+ Author Affiliations
- Author Affiliations
A Área Mejora y Biotecnología, IFAPA-Centro Alameda del Obispo, Apdo. 3092, 14080, Córdoba, Spain.
B Instituto de Agricultura Sostenible-CSIC, Alameda del Obispo s/n, 14004, Córdoba, Spain.
C Departament of Food Phytochemistry, Instituto de la Grasa (CSIC). Campus Universidad Pablo de Olavide, Edificio 46. Ctra. de Utrera, Km 1, E-41013 Sevilla, Spain.
D Corresponding author. Email: sgatienza@ias.csic.es
Crop and Pasture Science 70(7) 567-574 https://doi.org/10.1071/CP18531
Submitted: 19 November 2018 Accepted: 28 May 2019 Published: 25 July 2019
Abstract
A high carotenoid content is important for the production of pasta from durum wheat (Triticum durum Desf.) and yellow alkaline noodle from common wheat (T. aestivum L.). Carotenoid esters are more stable than free carotenoid during storage and processing, and thus they allow a higher retention through the food chain. Chromosome 7D carries gene(s) for lutein esterification. The aim of this study was the physical mapping of the gene(s) for lutein esterification on chromosome 7D and the identification of candidate genes for this trait. We developed crosses between a set of deletion lines for chromosome 7D in Chinese Spring (CS) background and the CS–Hordeum chilense substitution line CS(7D)7Hch. The F2 progeny derived from the deletion line 7DS4 produced a lower amount of lutein esters, which indicates that the main gene for lutein esterification is in the region of chromosome 7D lacking in 7DS4. Other gene(s) are contributing to lutein esterification because small amounts of lutein esters are produced in 7DS4. Genotyping by DArTSeq revealed that 7DS4 lacks a 127.7 Mb region of 7DS. A set of 10 candidate genes for lutein esterification was identified by using the wheat reference genome sequence along with the Wheat Expression Browser. This region contains the Lute locus previously identified in a different genetic background. Four genes with acyltransferase or GDSL esterase/lipase activity were identified in the vicinity of Lute. Our results indicate that the gene TraesCS7D01G094000 is a likely candidate for Lute but the gene TraesCS7D01G093200 cannot be ruled out. The candidate genes reported in this work are worthy for further investigation.
Additional keywords: lutein diesters, tritordeum.
References
Ahmad FT, Asenstorfer RE, Soriano IR, Mares DJ (2013) Effect of temperature on lutein esterification and lutein stability in wheat grain. Journal of Cereal Science 58, 408–413.| Effect of temperature on lutein esterification and lutein stability in wheat grain.Crossref | GoogleScholarGoogle Scholar |
Ahmad FT, Mather DE, Law H-Y, Li M, Yousif SA-J, Chalmers KJ, Asenstorfer RE, Mares DJ (2015) Genetic control of lutein esterification in wheat (Triticum aestivum L.) grain. Journal of Cereal Science 64, 109–115.
| Genetic control of lutein esterification in wheat (Triticum aestivum L.) grain.Crossref | GoogleScholarGoogle Scholar |
Akoh CC, Lee G-C, Liaw Y-C, Huang T-H, Shaw J-F (2004) GDSL family of serine esterases/lipases. Progress in Lipid Research 43, 534–552.
| GDSL family of serine esterases/lipases.Crossref | GoogleScholarGoogle Scholar | 15522763PubMed |
Alaux M, Rogers J, Letellier T, Flores R, Alfama F, Pommier C, Mohellibi N, Durand S, Kimmel E, Michotey C, Guerche C, Loaec M, Lainé M, Steinbach D, Choulet F, Rimbert H, Leroy P, Guilhot N, Salse J, Feuillet C, Paux E, Eversole K, Adam-Blondon A-F, Quesneville H, Consortium IWGS (2018) Linking the International Wheat Genome Sequencing Consortium bread wheat reference genome sequence to wheat genetic and phenomic data. Genome Biology 19, 111
| Linking the International Wheat Genome Sequencing Consortium bread wheat reference genome sequence to wheat genetic and phenomic data.Crossref | GoogleScholarGoogle Scholar | 30115101PubMed |
Ariizumi T, Kishimoto S, Kakami R, Maoka T, Hirakawa H, Suzuki Y, Ozeki Y, Shirasawa K, Bernillon S, Okabe Y, Moing A, Asamizu E, Rothan C, Ohmiya A, Ezura H (2014) Identification of the carotenoid modifying gene PALE YELLOW PETAL 1 as an essential factor in xanthophyll esterification and yellow flower pigmentation in tomato (Solanum lycopersicum). The Plant Journal 79, 453–465.
| Identification of the carotenoid modifying gene PALE YELLOW PETAL 1 as an essential factor in xanthophyll esterification and yellow flower pigmentation in tomato (Solanum lycopersicum).Crossref | GoogleScholarGoogle Scholar | 24888879PubMed |
Atienza SG, Ballesteros J, Martin A, Hornero-Mendez D (2007) Genetic variability of carotenoid concentration and degree of esterification among tritordeum (×Tritordeum Ascherson et Graebner) and durum wheat accessions. Journal of Agricultural and Food Chemistry 55, 4244–4251.
| Genetic variability of carotenoid concentration and degree of esterification among tritordeum (×Tritordeum Ascherson et Graebner) and durum wheat accessions.Crossref | GoogleScholarGoogle Scholar | 17439153PubMed |
Borrill P, Ramirez-Gonzalez R, Uauy C (2016) expVIP: a customizable RNA-seq data analysis and visualization platform. Plant Physiology 170, 2172–2186.
| expVIP: a customizable RNA-seq data analysis and visualization platform.Crossref | GoogleScholarGoogle Scholar | 26869702PubMed |
Camacho C, Coulouris G, Avagyan V, Ma N, Papadopoulos J, Bealer K, Madden TL (2009) BLAST+: architecture and applications. BMC Bioinformatics 10, 421
| BLAST+: architecture and applications.Crossref | GoogleScholarGoogle Scholar | 20003500PubMed |
Chen Q, Steinhauer L, Hammerlindl J, Keller W, Zou J (2007) Biosynthesis of phytosterol esters: identification of a sterol O-acyltransferase in Arabidopsis. Plant Physiology 145, 974–984.
| Biosynthesis of phytosterol esters: identification of a sterol O-acyltransferase in Arabidopsis.Crossref | GoogleScholarGoogle Scholar | 17885082PubMed |
Endo TR, Gill BS (1996) The deletion stocks of common wheat. The Journal of Heredity 87, 295–307.
| The deletion stocks of common wheat.Crossref | GoogleScholarGoogle Scholar |
Ficco DBM, Mastrangelo AM, Trono D, Borrelli GM, De Vita P, Fares C, Beleggia R, Platani C, Papa R (2014) The colours of durum wheat: a review. Crop & Pasture Science 65, 1–15.
| The colours of durum wheat: a review.Crossref | GoogleScholarGoogle Scholar |
Hagras AAA, Kishii M, Tanaka H, Sato K, Tsujimoto H (2005) Genomic differentiation of Hordeum chilense from H. vulgare as revealed by repetitive and EST sequences. Genes & Genetic Systems 80, 147–159.
| Genomic differentiation of Hordeum chilense from H. vulgare as revealed by repetitive and EST sequences.Crossref | GoogleScholarGoogle Scholar |
Howitt CA, Cavanagh CR, Bowerman AF, Cazzonelli C, Rampling L, Mimica JL, Pogson BJ (2009) Alternative splicing, activation of cryptic exons and amino acid substitutions in carotenoid biosynthetic genes are associated with lutein accumulation in wheat endosperm. Functional & Integrative Genomics 9, 363–376.
| Alternative splicing, activation of cryptic exons and amino acid substitutions in carotenoid biosynthetic genes are associated with lutein accumulation in wheat endosperm.Crossref | GoogleScholarGoogle Scholar |
Kaneko S, Nagamine T, Yamada T (1995) Esterification of endosperm lutein with fatty acids during the storage of wheat seeds. Bioscience, Biotechnology, and Biochemistry 59, 1–4.
| Esterification of endosperm lutein with fatty acids during the storage of wheat seeds.Crossref | GoogleScholarGoogle Scholar |
Lippold F, Dorp Kv, Abraham M, Hölzl G, Wewer V, Yilmaz JL, Lager I, Montandon C, Besagni C, Kessler F, Stymne S, Dörmann P (2012) Fatty acid phytyl ester synthesis in chloroplasts of Arabidopsis. The Plant Cell 24, 2001–2014.
| Fatty acid phytyl ester synthesis in chloroplasts of Arabidopsis.Crossref | GoogleScholarGoogle Scholar | 22623494PubMed |
Mares DJ, Campbell AW (2001) Mapping components of flour colour in Australian wheat. Australian Journal of Agricultural Research 52, 1297–1309.
| Mapping components of flour colour in Australian wheat.Crossref | GoogleScholarGoogle Scholar |
Mattera MG, Cabrera A, Hornero-Méndez D, Atienza SG (2015) Lutein esterification in wheat endosperm is controlled by the homoeologous group 7, and is increased by the simultaneous presence of chromosomes 7D and 7Hch from Hordeum chilense. Crop & Pasture Science 66, 912–921.
| Lutein esterification in wheat endosperm is controlled by the homoeologous group 7, and is increased by the simultaneous presence of chromosomes 7D and 7Hch from Hordeum chilense.Crossref | GoogleScholarGoogle Scholar |
Mattera MG, Hornero-Méndez D, Atienza SG (2017) Lutein ester profile in wheat and tritordeum can be modulated by temperature: Evidences for regioselectivity and fatty acid preferential of enzymes encoded by genes on chromosomes 7D and 7H(ch). Food Chemistry 219, 199–206.
| Lutein ester profile in wheat and tritordeum can be modulated by temperature: Evidences for regioselectivity and fatty acid preferential of enzymes encoded by genes on chromosomes 7D and 7H(ch).Crossref | GoogleScholarGoogle Scholar | 27765217PubMed |
Mellado-Ortega E, Hornero-Mendez D (2012) Isolation and identification of lutein esters, including their regioisomers, in tritordeum (×Tritordeum Ascherson et Graebner) grains: Evidence for a preferential xanthophyll acyltransferase activity. Food Chemistry 135, 1344–1352.
| Isolation and identification of lutein esters, including their regioisomers, in tritordeum (×Tritordeum Ascherson et Graebner) grains: Evidence for a preferential xanthophyll acyltransferase activity.Crossref | GoogleScholarGoogle Scholar | 22953864PubMed |
Mellado-Ortega E, Hornero-Méndez D (2016) Carotenoid evolution during short-storage period of durum wheat (Triticum turgidum conv. durum) and tritordeum (×Tritordeum Ascherson et Graebner) whole-grain flours. Food Chemistry 192, 714–723.
| Carotenoid evolution during short-storage period of durum wheat (Triticum turgidum conv. durum) and tritordeum (×Tritordeum Ascherson et Graebner) whole-grain flours.Crossref | GoogleScholarGoogle Scholar | 26304402PubMed |
Mellado-Ortega E, Hornero-Méndez D (2017) Effect of long-term storage on the free and esterified carotenoids in durum wheat (Triticum turgidum conv. durum) and tritordeum (×Tritordeum Ascherson et Graebner) grains. Food Research International 99, 877–890.
| Effect of long-term storage on the free and esterified carotenoids in durum wheat (Triticum turgidum conv. durum) and tritordeum (×Tritordeum Ascherson et Graebner) grains.Crossref | GoogleScholarGoogle Scholar | 28847425PubMed |
Mellado-Ortega E, Hornero-Méndez D (2018) Effect of lutein esterification on the differential distribution of carotenoids in germ and endosperm fractions from tritordeum grains. Journal of Cereal Science 79, 462–468.
| Effect of lutein esterification on the differential distribution of carotenoids in germ and endosperm fractions from tritordeum grains.Crossref | GoogleScholarGoogle Scholar |
Mellado-Ortega E, Atienza SG, Hornero-Méndez D (2015) Carotenoid evolution during postharvest storage of durum wheat (Triticum turgidum conv. durum) and tritordeum (×Tritordeum Ascherson et Graebner) grains. Journal of Cereal Science 62, 134–142.
| Carotenoid evolution during postharvest storage of durum wheat (Triticum turgidum conv. durum) and tritordeum (×Tritordeum Ascherson et Graebner) grains.Crossref | GoogleScholarGoogle Scholar |
Mínguez-Mosquera MI, Hornero-Méndez D (1993) Separation and quantification of the carotenoid pigments in red peppers (Capsicum annuum L.), paprika and oleoresin by reversed-phase HPLC. Journal of Agricultural and Food Chemistry 41, 1616–1620.
| Separation and quantification of the carotenoid pigments in red peppers (Capsicum annuum L.), paprika and oleoresin by reversed-phase HPLC.Crossref | GoogleScholarGoogle Scholar |
Murray YHG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research 8, 4321–4326.
| Rapid isolation of high molecular weight plant DNA.Crossref | GoogleScholarGoogle Scholar |
Paznocht L, Kotíková Z, Šulc M, Lachman J, Orsák M, Eliášová M, Martinek P (2018) Free and esterified carotenoids in pigmented wheat, tritordeum and barley grains. Food Chemistry 240, 670–678.
| Free and esterified carotenoids in pigmented wheat, tritordeum and barley grains.Crossref | GoogleScholarGoogle Scholar | 28946328PubMed |
Ramírez-González RH, Borrill P, Lang D, Harrington SA, Brinton J, Venturini L, Davey M, Jacobs J, van Ex F, Pasha A, Khedikar Y, Robinson SJ, Cory AT, Florio T, Concia L, Juery C, Schoonbeek H, Steuernagel B, Xiang D, Ridout CJ, Chalhoub B, Mayer KFX, Benhamed M, Latrasse D, Bendahmane A, Wulff BBH, Appels R, Tiwari V, Datla R, Choulet F, Pozniak CJ, Provart NJ, Sharpe AG, Paux E, Spannagl M, Bräutigam A, Uauy C (2018) The transcriptional landscape of polyploid wheat. Science 361,
| The transcriptional landscape of polyploid wheat.Crossref | GoogleScholarGoogle Scholar | 30115783PubMed |
Rodríguez-Suárez C, Mellado-Ortega E, Hornero-Méndez D, Atienza S (2014) Increase in transcript accumulation of Psy1 and e-Lcy genes in grain development is associated with differences in seed carotenoid content between durum wheat and tritordeum. Plant Molecular Biology 84, 659–673.
| Increase in transcript accumulation of Psy1 and e-Lcy genes in grain development is associated with differences in seed carotenoid content between durum wheat and tritordeum.Crossref | GoogleScholarGoogle Scholar | 24306494PubMed |
Subagio A, Wakaki H, Morita N (1999) Stability of lutein and its myristate esters. Bioscience, Biotechnology, and Biochemistry 63, 1784–1786.
| Stability of lutein and its myristate esters.Crossref | GoogleScholarGoogle Scholar | 26300170PubMed |
Wijaya GY, Ingram C, Asenstorfer RE, Mares DJ (2016) Contribution of apigenin di-C-glycosides and lutein to the colour of yellow alkaline noodles. Crop & Pasture Science 67, 594–604.
| Contribution of apigenin di-C-glycosides and lutein to the colour of yellow alkaline noodles.Crossref | GoogleScholarGoogle Scholar |
