A linked SNP marker to genotype Fr-B2 in wheat
H. A. Eagles
A B , J. Hyles A G , Jayne Wilson C , Karen Cane C D , K. L. Forrest E , M. J. Hayden E F , K. Ramm A and Ben Trevaskis A
+ Author Affiliations
- Author Affiliations
A CSIRO Agriculture and Food, GPO Box 1700, Canberra, ACT 2601, Australia.
B Mailing address: 3 Tacoma Boulevard, Pasadena, SA 5042, Australia.
C Department of Economic Development, Jobs, Transport and Resources, PB260, Horsham, Vic. 3401, Australia.
D Mailing address: 18 Pindari Drive, Mt Clear, Vic. 3350, Australia.
E Department of Economic Development, Jobs, Transport and Resources, Agriculture Victoria Research, Agribio Centre, La Trobe Research and Development Park, Bundoora, Vic. 3083, Australia.
F School of Applied Biology, La Trobe University, Bundoora, Vic. 2086, Australia.
G Corresponding author. Email: jessica.hyles@csiro.au
Crop and Pasture Science 69(9) 859-863 https://doi.org/10.1071/CP18248
Submitted: 01 June 2018 Accepted: 18 July 2018 Published: 24 August 2018
Abstract
Fr-B2 is a complex locus on chromosome 5B that affects frost tolerance, days to heading, grain yield and probably other traits of commercial importance in wheat (Triticum aestivum L.). It interacts epistatically with other major genes, especially VRN1. There are two known alleles of Fr-B2: an intact, wild-type allele, and an allele with a large deletion. Published methods for identifying these alleles are slow and expensive, making the development of a high-throughput, co-dominant SNP (single-nucleotide polymorphism) marker highly desirable, especially for commercial wheat breeding.
A diverse panel of cultivars and breeding lines was characterised for SNPs and alleles of Fr-B2. Four SNP markers co-segregated as a haplotype block with Fr-B2 across unrelated cultivars and related backcrosses differing for alleles of Fr-B2. A robust KASP (Kompetitive allele-specific PCR) assay was developed for one of the SNPs, KASP_IWB26333, which should facilitate the inclusion of Fr-B2 on genotyping platforms for breeding and research.
Additional keywords: marker-assisted breeding, pleiotropy, SNP–gene co-segregation.
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