A cytological and molecular analysis of D-genome chromosome retention following F2–F6 generations of hexaploid × tetraploid wheat crosses
Sriram Padmanaban A , Peng Zhang B , Mark W. Sutherland A , Noel L. Knight A C and Anke Martin A D
+ Author Affiliations
- Author Affiliations
A Centre for Crop Health, University of Southern Queensland, Toowoomba, Qld 4350, Australia.
B Plant Breeding Institute, The University of Sydney, Cobbitty, NSW 2570, Australia.
C Present address: School of Integrative Plant Science, Plant Pathology and Plant–Microbe Biology Section, Cornell University, Geneva, NY 14456, USA.
D Corresponding author. Email: anke.martin@usq.edu.au
Crop and Pasture Science 69(2) 121-130 https://doi.org/10.1071/CP17240
Submitted: 7 July 2017 Accepted: 26 November 2017 Published: 5 February 2018
Abstract
Both hexaploid bread wheat (AABBDD) (Triticum aestivum L.) and tetraploid durum wheat (AABB) (T. turgidum spp. durum) are highly significant global food crops. Crossing these two wheats with different ploidy levels results in pentaploid (AABBD) F1 lines. This study investigated the differences in the retention of D chromosomes between different hexaploid × tetraploid crosses in subsequent generations by using molecular and cytological techniques. Significant differences (P < 0.05) were observed in the retention of D chromosomes in the F2 generation depending on the parents of the original cross. One of the crosses, 2WE25 × 950329, retained at least one copy of each D chromosome in 48% of its F2 lines. For this cross, the retention or elimination of D chromosomes was determined through several subsequent self-fertilised generations. Cytological analysis indicated that D chromosomes were still being eliminated at the F5 generation, suggesting that in some hexaploid × tetraploid crosses, D chromosomes are unstable for many generations. This study provides information on the variation in D chromosome retention in different hexaploid × tetraploid wheat crosses and suggests efficient strategies for utilising D genome retention or elimination to improve bread and durum wheat, respectively.
Additional keywords: DArTseq, FISH, GISH, interploidy crosses.
References
Bovill WD, Horne M, Herde D, Davis M, Wildermuth GB, Sutherland M (2010) Pyramiding QTL increases seedling resistance to crown rot (Fusarium pseudograminearum) of wheat (Triticum aestivum). Theoretical and Applied Genetics 121, 127–136.| Pyramiding QTL increases seedling resistance to crown rot (Fusarium pseudograminearum) of wheat (Triticum aestivum).Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC3czjs1SmtQ%3D%3D&md5=feac0e39fb6e830eda7d0ad3c0dab236CAS |
Eberhard FS, Zhang P, Lehmensiek A, Hare RA, Simpfendorfer S, Sutherland MW (2010) Chromosome composition of an F2 Triticum aestivum × T. turgidum spp. durum cross analysed by DArT markers and MCFISH. Crop & Pasture Science 61, 619–624.
| Chromosome composition of an F2 Triticum aestivum × T. turgidum spp. durum cross analysed by DArT markers and MCFISH.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXhtVWqu7rN&md5=1914215f6a8bbd923fe40fd4fefade64CAS |
Gilbert J, Procunier J, Aung T (2000) Influence of the D genome in conferring resistance to fusarium head blight in spring wheat. Euphytica 114, 181–186.
| Influence of the D genome in conferring resistance to fusarium head blight in spring wheat.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXntFOgsL4%3D&md5=783106d2d6369f541ca3561fa1e76301CAS |
Kalous J, Martin J, Sherman J, Heo H-Y, Blake N, Lanning S, Eckhoff J, Chao S, Akhunov E, Talbert L (2015) Impact of the D genome and quantitative trait loci on quantitative traits in a spring durum by spring bread wheat cross. Theoretical and Applied Genetics 128, 1799–1811.
| Impact of the D genome and quantitative trait loci on quantitative traits in a spring durum by spring bread wheat cross.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2MXpslGls7Y%3D&md5=d6a68f7e889dfff2ef694e2809357a8bCAS |
Kihara H (1924) ‘Cytologische und genetische Studien bei wichtigen Getreidearten mit besondere Riichsicht auf das Verhalten der Chromosomen und die Sterilitat in den Bastarden.’ Memoirs of the College of Science, University of Kyoto. (Faculty of Science, University of Kyoto: Kyoto, Japan)
Kihara H (1982) ‘Wheat studies: retrospect and prospects.’ (Kodansha Ltd: Tokyo)
King J, Armstead I, Harper J, Ramsey L, Snape J, Waugh R, James C, Thomas A, Gasior D, Kelly R (2013) Exploitation of interspecific diversity for monocot crop improvement. Heredity 110, 475–483.
| Exploitation of interspecific diversity for monocot crop improvement.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXmt1Ohur4%3D&md5=af464e3034aed8f95a0e185f7218ae0dCAS |
Koo D-H, Sehgal SK, Friebe B, Gill BS (2015) Structure and stability of telocentric chromosomes in wheat. PLoS One 10, e0137747
| Structure and stability of telocentric chromosomes in wheat.Crossref | GoogleScholarGoogle Scholar |
Lanning SP, Blake NK, Sherman JD, Talbert LE (2008) Variable production of tetraploid and hexaploid progeny lines from spring wheat by durum wheat crosses. Crop Science 48, 199–202.
| Variable production of tetraploid and hexaploid progeny lines from spring wheat by durum wheat crosses.Crossref | GoogleScholarGoogle Scholar |
Lim K-B, Ramanna MS, Jacobsen E, van Tuyl JM (2003) Evaluation of BC2 progenies derived from 3x-2x and 3x-4x crosses of Lilium hybrids: a GISH analysis. Theoretical and Applied Genetics 106, 568–574.
| Evaluation of BC2 progenies derived from 3x-2x and 3x-4x crosses of Lilium hybrids: a GISH analysis.Crossref | GoogleScholarGoogle Scholar |
Martin A, Simpfendorfer S, Hare RA, Eberhard FS, Sutherland MW (2011) Retention of D genome chromosomes in pentaploid wheat crosses. Heredity 107, 315–319.
| Retention of D genome chromosomes in pentaploid wheat crosses.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXht1ajt7bP&md5=37d2bf4b7e4c6c1fc4221792c8a08314CAS |
Martin A, Simpfendorfer S, Hare R, Sutherland M (2013) Introgression of hexaploid sources of crown rot resistance into durum wheat. Euphytica 192, 463–470.
| Introgression of hexaploid sources of crown rot resistance into durum wheat.Crossref | GoogleScholarGoogle Scholar |
Mukai Y, Nakahara Y, Yamamoto M (1993) Simultaneous discrimination of the three genomes in hexaploid wheat by multicolor fluorescence in situ hybridization using total genomic and highly repeated DNA probes. Genome 36, 489–494.
| Simultaneous discrimination of the three genomes in hexaploid wheat by multicolor fluorescence in situ hybridization using total genomic and highly repeated DNA probes.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3sXms1emurg%3D&md5=d0221106a12b61c22434de82895f3144CAS |
Munns R, Hare R, James R, Rebetzke G (2000) Genetic variation for improving the 648 salt tolerance of durum wheat. Australian Journal of Agricultural Research 51, 69–74.
| Genetic variation for improving the 648 salt tolerance of durum wheat.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXks12htg%3D%3D&md5=9e9a9aad574df86750008ca862eb325bCAS |
Padmanaban S, Sutherland MW, Knight NL, Martin A (2017a) Genome inheritance in populations derived from hexaploid/tetraploid and tetraploid/hexaploid wheat crosses. Molecular Breeding 37, 48
| Genome inheritance in populations derived from hexaploid/tetraploid and tetraploid/hexaploid wheat crosses.Crossref | GoogleScholarGoogle Scholar |
Padmanaban S, Zhang P, Hare RA, Sutherland MW, Martin A (2017b) Pentaploid wheat hybrids: Applications, characterisation, and challenges. Frontiers in Plant Science 8, 358
| Pentaploid wheat hybrids: Applications, characterisation, and challenges.Crossref | GoogleScholarGoogle Scholar |
Rayburn AL, Gill BS (1986) Isolation of a D-genome specific repeated DNA sequence from Aegilops squarrosa. Plant Molecular Biology Reporter 4, 102–109.
| Isolation of a D-genome specific repeated DNA sequence from Aegilops squarrosa.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL2sXhtl2msg%3D%3D&md5=57a6e57f74ae0ab4830cc219535a4f57CAS |
Ren R, Ray R, Li P, Xu J, Zhang M, Liu G, Yao X, Kilian A, Yang X (2015) Construction of a high-density DArTseq SNP-based genetic map and identification of genomic regions with segregation distortion in a genetic population derived from a cross between feral and cultivated-type watermelon. Molecular Genetics and Genomics 290, 1457–1470.
| Construction of a high-density DArTseq SNP-based genetic map and identification of genomic regions with segregation distortion in a genetic population derived from a cross between feral and cultivated-type watermelon.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2MXjtFCitLc%3D&md5=ccd892cbc00deb08da1c8e3a0be2b3aeCAS |
Riley R, Chapman V (1967) The inheritance in wheat of crossability with rye. Genetical Research 9, 259–267.
| The inheritance in wheat of crossability with rye.Crossref | GoogleScholarGoogle Scholar |
Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard R (1984) Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proceedings of the National Academy of Sciences of the United States of America 81, 8014–8018.
| Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL2MXptlWitg%3D%3D&md5=cf3ce480ad9ef20eaf34e64d53653f52CAS |
Schwarzacher T, Leitch A, Bennett M, Heslop-Harrison J (1989) In situ localization of parental genomes in a wide hybrid. Annals of Botany 64, 315–324.
| In situ localization of parental genomes in a wide hybrid.Crossref | GoogleScholarGoogle Scholar |
Sharma HC, Gill BS (1983) Current status of wide hybridization in wheat. Euphytica 32, 17–31.
Sheedy JG, McKay AC, Lewis J, Vanstone VA, Fletcher S, Kelly A, Thompson JP (2015) Cereal cultivars can be ranked consistently for resistance to root-lesion nematodes (Pratylenchus thornei & P. neglectus) using diverse procedures. Australasian Plant Pathology 44, 175–182.
| Cereal cultivars can be ranked consistently for resistance to root-lesion nematodes (Pratylenchus thornei & P. neglectus) using diverse procedures.Crossref | GoogleScholarGoogle Scholar |
Wang H, Liu D, Yan Z, Wei Y, Zheng Y (2005) Cytological characteristics of F2 hybrids between Triticum aestivum L. and T. durum Desf with reference to wheat breeding. Journal of Applied Genetics 46, 365–369.
Xu L, Wang M, Cheng P, Kang Z, Hulbert S, Chen X (2013) Molecular mapping of Yr53, a new gene for stripe rust resistance in durum wheat accession PI 480148 and its transfer to common wheat. Theoretical and Applied Genetics 126, 523–533.
| Molecular mapping of Yr53, a new gene for stripe rust resistance in durum wheat accession PI 480148 and its transfer to common wheat.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXhslakuro%3D&md5=d0713da627cc3408cb38705b427f435aCAS |
Zhang P, Li W, Friebe B, Gill BS (2004) Simultaneous painting of three genomes in hexaploid wheat by BAC-FISH. Genome 47, 979–987.
| Simultaneous painting of three genomes in hexaploid wheat by BAC-FISH.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXhtVyksb7F&md5=c6a8038cdee35a2b805b8a8e88930e7eCAS |
