Assessing genetic potential of elite interspecific and intraspecific advanced lentil lines for agronomic traits and their reaction to rust (Uromyces viciae-fabae)
Madhu Kumari A , Raj Kumar Mittal A , Rakesh Kumar Chahota B , Kalpna Thakur A , Swaran Lata A and Dorin Gupta A C D
+ Author Affiliations
- Author Affiliations
A Department of Crop Improvement, CSK Himachal Pradesh Agriculture University, Palampur, HP 176 062, India.
B Department of Agricultural Biotechnology, CSK Himachal Pradesh Agriculture University, Palampur, HP 176 062, India.
C School of Agriculture and Food, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Melbourne, Vic. 3010, Australia.
D Corresponding author. Email: dorin.gupta@unimelb.edu.au
Crop and Pasture Science 69(10) 999-1008 https://doi.org/10.1071/CP17145
Submitted: 6 April 2017 Accepted: 9 August 2018 Published: 3 September 2018
Abstract
The narrow genetic base of lentil (Lens culinaris) has challenged the efforts of breeders to increase its productivity under changing environmental conditions. Inclusion of wild species and diverse cultivated genotypes offers an opportunity to generate new variation through wide hybridisation to broaden the genetic base of cultivated lentil. We evaluated 96 elite, interspecific (L. culinaris × L. orientalis) and intraspecific advanced lentil genotypes along with four checks to determine the extent of genetic variation, resistance to lentil rust (Uromyces viciae-fabae), and the nature and magnitude of their genetic divergence. Sufficient genetic variability was revealed for all of the traits. High heritability and genetic advance were recorded for number of seeds per pod, number of pods per plant, seed yield per plant and biomass per plant. A positive correlation was recorded between grain yield and ten important plant traits. Statistical (D2) and molecular analyses grouped all genotypes into two main clusters and revealed sufficient genetic diversity among advanced lines. Our study showed promising results for creating new variation through wide hybridisation and identified lines L-354 and L-437-1 (rust-resistant) and HPLL-32 (moderately rust-resistant) superior for seed yield and related traits.
Additional keywords: interspecific hybridisation, PCR, sustainability.
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