The Chinese bread wheat cultivar Xiaoyanmai 7 harbours genes encoding a pair of novel high-molecular-weight glutenin subunits inherited from cereal rye
Bo Feng A , Francesco Sestili B , Stefania Masci B , Benedetta Margiotta C , Zhibin Xu A , Zujun Yang D , Chao Xiang A , Chunhong Zhou A , Domenico Lafiandra B E and Tao Wang A E
+ Author Affiliations
- Author Affiliations
A Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China.
B Department of Agriculture, Forestry, Nature & Energy, University of Tuscia, 01100 Viterbo, Italy.
C Institute of Biosciences and Bioresources, CNR, 70126 Bari, Italy.
D School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, China.
E Corresponding authors. Email: lafiandr@unitus.it; wangtao@cib.ac.cn
Crop and Pasture Science 67(1) 29-36 https://doi.org/10.1071/CP15191
Submitted: 10 June 2015 Accepted: 31 July 2015 Published: 29 January 2016
Abstract
The high-molecular-weight glutenin subunits (HMW-GS) represent a major component of the endosperm storage protein in the grains of wheat and its related species. Their technological importance results from their ready formation of intermolecular disulfide bonds, which underlie much of the visco-elasticity displayed by gluten and hence the processing quality of the flour. Here, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis revealed that the Chinese wheat cultivar Xiaoyanmai 7 formed four distinct HMW-GS, two of which are likely the product of a known allele at the Glu-B1 locus, whereas the other two did not match any known HMW-GS. A combined analysis based on reversed-phase high-performance liquid chromatography (RP-HPLC), N-terminal sequencing and mass spectrometry confirmed that the two novel proteins were genuine HMW-GS. Inspection of the DNA sequences showed that one of the novel HMW-GS was encoded by an x-type and the other by a y-type secalin gene. A karyotypic analysis confirmed that six of the seven pairs of Xiaoyanmai 7’s D genome chromosomes (the exception was chromosome 2D) had been replaced by rye chromosomes. The y-type HMW secalin present in Xiaoyanmai 7 differed from the standard By and Dy HWM-GS by the presence of an additional cysteine residue in its C-terminal domain.
Additional keywords: GISH, FISH, wheat–rye hybrid.
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