Embryogenesis and plant regeneration of the perennial pasture and medicinal legume Bituminaria bituminosa (L.) C.H. Stirton
M. Pazos-Navarro A , J. S. Croser B , M. Castello B , P. Ramankutty C , K. Heel D , D. Real B C E , D. J. Walker A , E. Correal A and M. Dabauza A F
+ Author Affiliations
- Author Affiliations
A Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA), C/Mayor s/n, 30150-La Alberca, Murcia, Spain.
B Centre for Plant Genetics and Breeding, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.
C School of Plant Biology, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.
D Centre for Microscopy, Characterisation and Analysis, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.
E Department of Agriculture and Food, Western Australia, South Perth, WA 6151, Australia.
F Corresponding author. Email: mercedes.dabauza@carm.es
Crop and Pasture Science 65(9) 934-943 https://doi.org/10.1071/CP14083
Submitted: 17 March 2014 Accepted: 19 June 2014 Published: 15 August 2014
Abstract
Bituminaria bituminosa (common name tedera) is a drought-tolerant perennial pasture species of agronomic and pharmaceutical interest for Mediterranean climates. Considering the importance of this legume, in vitro experiments were conducted to develop protocols for plant regeneration from embryogenic calli of leaves, petioles and anthers to efficiently exploit and maintain selected important clones from the tedera breeding program. The type of explant was a key factor in the frequency of embryogenesis and the number of embryos per callus. For plant regeneration from cultured anthers, appropriate anther physiological state (uninucleate stage of microsporogenesis), stress treatments (electroporation, 25 Ω, 25 µF, 1500 V) and culture conditions were determined. A robust flow-cytometry method was developed to analyse the ploidy status of callus, in vitro shoots and in vivo acclimatised plants derived from anther and leaf explants.
Additional keywords: androgenesis, flow cytometry, forage, furanocoumarins, haploid, somatic embryos.
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