Free Standard AU & NZ Shipping For All Book Orders Over $80!
Register      Login
Crop and Pasture Science Crop and Pasture Science Society
Plant sciences, sustainable farming systems and food quality
RESEARCH ARTICLE

Do lignite-derived organic amendments improve early-stage pasture growth and key soil biological and physicochemical properties?

Karen R. Little A C , Michael T. Rose A , William R. Jackson A , Timothy R. Cavagnaro B and Antonio F. Patti A
+ Author Affiliations
- Author Affiliations

A School of Chemistry, Monash University, Clayton, Vic. 3800, Australia.

B School of Agriculture, Food and Wine, University of Adelaide, Waite Campus, PMB1 Glen Osmond, SA, 5064, Australia.

C Corresponding author. Email: karen.little@monash.edu

Crop and Pasture Science 65(9) 899-910 https://doi.org/10.1071/CP13433
Submitted: 9 December 2013  Accepted: 4 June 2014   Published: 26 August 2014

Abstract

Commercial products derived from lignite (brown coal), sold mainly as humate preparations, are widely promoted as plant growth stimulants leading to higher crop yields. These products are also claimed to improve key indicators of soil health including soil pH and microbial biomass. In a glasshouse setting, we investigated the effect of six lignite-derived amendments applied at the manufacturer’s recommended rate on the early-stage growth of two pasture species, lucerne (Medicago sativa L.) and ryegrass (Lolium multiflorum Lam.). We used two soil types common to south-eastern Australia, and following an 8-week growing period, assessed soil pH, microbial biomass carbon and mycorrhizal colonisation as key indicators of soil health. We hypothesised that humic acid (HA) and macronutrients derived from the products would positively influence pasture growth and soil health indicators. Although significant growth effects were observed in response to some products, the effects were inconsistent across pasture and soil types. Treatment effects on tissue nutrient accumulation were rare, with the exception of increased potassium in ryegrass in one soil amended with raw brown coal, and decreased nitrogen in lucerne in the same soil amended with a granulated, slow-release humate product. Further, we found no consistent trends in mycorrhizal colonisation or microbial biomass carbon in response to individual treatments. Given the variable responses of the plant species and soil types to the amendments used here, we emphasise the need for further mechanistic studies to help understand how these amendments can be used to greatest effect.

Additional keywords: agronomy, alfalfa, amendment, humic, legume, organic, ryegrass.


References

Adani F, Genevini P, Zaccheo P, Zocchi G (1998) The effect of commercial humic acid on tomato plant growth and mineral nutrition. Journal of Plant Nutrition 21, 561–575.
The effect of commercial humic acid on tomato plant growth and mineral nutrition.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1cXhvVOls7k%3D&md5=c6ab9ee5127ade66965fcb525615c69eCAS |

Akinremi OO, Janzen HH, Lemke RL, Larney FJ (2000) Response of canola, wheat and green beans to leonardite additions. Canadian Journal of Soil Science 80, 437–443.
Response of canola, wheat and green beans to leonardite additions.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXnsVCltrw%3D&md5=47874b7d0c987247b36bd6494a0adc85CAS |

Alagöz Z, Yilmaz E (2009) Effects of different sources of organic matter on soil aggregate formation and stability: A laboratory study on a Lithic Rhodoxeralf from Turkey. Soil & Tillage Research 103, 419–424.
Effects of different sources of organic matter on soil aggregate formation and stability: A laboratory study on a Lithic Rhodoxeralf from Turkey.Crossref | GoogleScholarGoogle Scholar |

Albayrak S, Camas N (2005) Effects of difference levels and application times of humic acid on root and leaf yield and yield components of forage turnip (Brassica rapa L.). Journal of Agronomy 4, 130–133.
Effects of difference levels and application times of humic acid on root and leaf yield and yield components of forage turnip (Brassica rapa L.).Crossref | GoogleScholarGoogle Scholar |

Arancon NQ, Edwards CA, Lee S, Byrne R (2006) Effects of humic acids from vermicomposts on plant growth. European Journal of Soil Biology 42, S65–S69.
Effects of humic acids from vermicomposts on plant growth.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28Xht12ktLrP&md5=b1fbed4afdd919a827897ce61c624df0CAS |

Ascaso C, Fortun C, Rapsch S (1985) Action of humic acid preparations on leaf development, mineral elements contents and chloroplast ultrastructure of ryegrass plants. Photosynthetica 19, 294–299.

Asenjo MCG, González JL, Maldonado JM (2000) Influence of humic extracts on germination and growth of ryegrass. Communications in Soil Science and Plant Analysis 31, 101–114.
Influence of humic extracts on germination and growth of ryegrass.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXhtlyls7Y%3D&md5=ac1c6b8f359610bc28ad64bf92c23eaaCAS |

Asghari HR, Cavagnaro TR (2012) Arbuscular mycorrhizas reduce nitrogen loss via leaching. PLoS ONE 7, e29825
Arbuscular mycorrhizas reduce nitrogen loss via leaching.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38Xht12mtbs%3D&md5=f60ecbd7ebd8fd22e0da5e5b2f247c76CAS | 22253790PubMed |

Atiyeh RM, Lee S, Edwards CA, Arancon NQ, Metzger JD (2002) The influence of humic acids derived from earthworm-processed organic wastes on plant growth. Bioresource Technology 84, 7–14.
The influence of humic acids derived from earthworm-processed organic wastes on plant growth.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XjtVSltb8%3D&md5=0f94fa9785e4a4facfdbfc3112ac2777CAS | 12137272PubMed |

Bidegain RA, Kaemmerer M, Guirrse M, Hafidi M, Rey F, Morard P, Revel JC (2000) Effects of humic substances from composted or chemically decomposed poplar sawdust on mineral nutrition of ryegrass. The Journal of Agricultural Science 134, 259–267.
Effects of humic substances from composted or chemically decomposed poplar sawdust on mineral nutrition of ryegrass.Crossref | GoogleScholarGoogle Scholar |

Canarutto S, Pera A, Lamarca M, Vallini G (1996) Effects of humic acids from compost-stabilized green waste or leonardite on soil shrinkage and microaggregation. Compost Science & Utilization 4, 40–46.
Effects of humic acids from compost-stabilized green waste or leonardite on soil shrinkage and microaggregation.Crossref | GoogleScholarGoogle Scholar |

Çelik H, Katkat AV, Aşık BB, Turan MA (2011) Effect of foliar-applied humic acid to dry weight and mineral nutrient uptake of maize under calcareous soil conditions. Communications in Soil Science and Plant Analysis 42, 29–38.
Effect of foliar-applied humic acid to dry weight and mineral nutrient uptake of maize under calcareous soil conditions.Crossref | GoogleScholarGoogle Scholar |

Chan KY (2010) Assessing P fertiliser use in vegetable production: Agronomic and environmental implications. Australian Journal of Soil Research 48, 674–681.
Assessing P fertiliser use in vegetable production: Agronomic and environmental implications.Crossref | GoogleScholarGoogle Scholar |

Chen Y, Clapp CE, Magan H (2004a) Mechanisms of plant growth stimulation by humic substances: the role of organo-iron complexes. Soil Science and Plant Nutrition 50, 1089–1095.
Mechanisms of plant growth stimulation by humic substances: the role of organo-iron complexes.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXjslSktA%3D%3D&md5=b7b66062079669bf75ae546558ae691dCAS |

Chen Y, De N, Maria, Aviad T (2004b) Stimulatory effects of humic substances on plant growth. In ‘Soil organic matter in sustainable agriculture’ . (CRC Press: Boca Raton, FL, USA)

Çimrin K, Karaca D, Bozkurt M (2001) The effect of NPK and humic acid applications on growth and nutrition of corn plant (Zea mays L.). Ankara University Journal of Agricultural Science 7, 95–100.

Demirbas A, Kar Y, Deveci H (2006) Humic substances and nitrogen-containing compounds from low rank brown coals. Energy Sources, Part A: Recovery, Utilization, and Environmental Effects 28, 341–351.
Humic substances and nitrogen-containing compounds from low rank brown coals.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XptFKqug%3D%3D&md5=1daac51376c808a33d24cd00162d1ad9CAS |

Duval JR, Dainello FJ, Haby VA, Earhart DR (1998) Evaluating leonardite as a crop growth enhancer for turnip and mustard greens. HortTechnology 8, 564–567.

Eyheraguibel B, Silvestre J, Morard P (2008) Effects of humic substances derived from organic waste enhancement on the growth and mineral nutrition of maize. Bioresource Technology 99, 4206–4212.
Effects of humic substances derived from organic waste enhancement on the growth and mineral nutrition of maize.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXjtl2nsrk%3D&md5=3e176ca96ac4269ca0bab3e4bac4c056CAS | 17962015PubMed |

Fagbenro JA, Agboola AA (1993) Effect of different levels of humic acid on the growth and nutrient uptake of teak seedlings. Journal of Plant Nutrition 16, 1465–1483.
Effect of different levels of humic acid on the growth and nutrient uptake of teak seedlings.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3sXlvVSjsLc%3D&md5=c4bd2330abb1be0c64a54c35cf9d2dc8CAS |

Fageria NK (2010) ‘The use of nutrients in crop plants.’ (Taylor & Francis: Oxford, UK)

Feibert EB, Shock CC, Saunders LD (2003) Nonconventional additives leave onion yield and quality unchanged. HortScience 38, 381–386.

Ferreras L, Gomez E, Toresani S, Firpo I, Rotondo R (2006) Effect of organic amendments on some physical, chemical and biological properties in a horticultural soil. Bioresource Technology 97, 635–640.
Effect of organic amendments on some physical, chemical and biological properties in a horticultural soil.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXht12jur3N&md5=f7ea53e1285b8f554e02bb3212e301f1CAS | 15905087PubMed |

Giovannetti M, Mosse B (1980) An evaluation of techniques for measuring vesicular arbuscular mycorrhizal infection in roots. New Phytologist 84, 489–500.
An evaluation of techniques for measuring vesicular arbuscular mycorrhizal infection in roots.Crossref | GoogleScholarGoogle Scholar |

Gryndler M, Hršelová H, Sudová R, Gryndlerová H, Řezáčová V, Merhautová V (2005) Hyphal growth and mycorrhiza formation by the arbuscular mycorrhizal fungus Glomus claroideum BEG 23 is stimulated by humic substances. Mycorrhiza 15, 483–488.
Hyphal growth and mycorrhiza formation by the arbuscular mycorrhizal fungus Glomus claroideum BEG 23 is stimulated by humic substances.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXhtFemur3E&md5=a013a0c15f629883a4f74c9078a70d91CAS | 15830211PubMed |

Hartz TK (2010) Humic substances generally ineffective in improving vegetable crop nutrient uptake or productivity. HortScience 45, 906–910.

Hayes TM, Hayes MHB, Skjemstad JO, Swift RS (2008) Compositional relationships between organic matter in a grassland soil and its drainage waters. European Journal of Soil Science 59, 603–616.
Compositional relationships between organic matter in a grassland soil and its drainage waters.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXhtVGls7fJ&md5=230cd567c8f1450605a441d3aac74c4aCAS |

Hiltbrunner D, Schulze S, Hagedorn F, Schmidt MWI, Zimmmermann S (2012) Cattle trampling alters soil properties and changes soil microbial communities in a Swiss sub-alpine pasture. Geoderma 170, 369–377.
Cattle trampling alters soil properties and changes soil microbial communities in a Swiss sub-alpine pasture.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38Xhsl2gsL8%3D&md5=0c5ef43dadad3a40991e9f9e4b87e6e1CAS |

Hoben JP, Gehl RJ, Millar N, Grace PR, Robertson GP (2011) Nonlinear nitrous oxide (N2O) response to nitrogen fertilizer in on-farm corn crops of the US Midwest. Global Change Biology 17, 1140–1152.
Nonlinear nitrous oxide (N2O) response to nitrogen fertilizer in on-farm corn crops of the US Midwest.Crossref | GoogleScholarGoogle Scholar |

Houlbrooke DJ (2011) Land-use intensification in New Zealand: Effects on soil properties and pasture production. The Journal of Agricultural Science 149, 337–349.
Land-use intensification in New Zealand: Effects on soil properties and pasture production.Crossref | GoogleScholarGoogle Scholar |

Imbufe AU, Patti AF, Burrow D, Surapaneni A, Jackson WR, Milner AD (2005) Effects of potassium humate on aggregate stability of two soils from Victoria, Australia. Geoderma 125, 321–330.
Effects of potassium humate on aggregate stability of two soils from Victoria, Australia.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXhs12gsr0%3D&md5=108b3e829d239af2a30cb96ebd0dadf0CAS |

Isbell RF (2002) ‘The Australian Soil Classification.’ Revised edn (CSIRO Publishing: Melbourne)

Jackson LE, Burger M, Cavagnaro TR (2008) Roots, Nitrogen Transformations, and Ecosystem Services. Annual Review of Plant Biology 59, 341–363.
Roots, Nitrogen Transformations, and Ecosystem Services.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXntFaqsb4%3D&md5=5b8d24e13c4bfa190de7e3ea9efd062eCAS | 18444903PubMed |

Kremen C, Miles A (2012) Ecosystem services in biologically diversified versus conventional farming systems: Benefits, externalities, and trade-offs. Ecology and Society 17,
Ecosystem services in biologically diversified versus conventional farming systems: Benefits, externalities, and trade-offs.Crossref | GoogleScholarGoogle Scholar |

Kunkel R, Holstad N (1968) Effects of adding humates to the fertilizer on the yield and quality of Russet Burbank potatoes. American Potato Journal 45, 449–457.
Effects of adding humates to the fertilizer on the yield and quality of Russet Burbank potatoes.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaF1MXhtVGnur0%3D&md5=b785930e051f9cf089f618bc32de0b45CAS |

Lee YS, Bartlett RJ (1976) Stimulation of plant growth by humic substances. Soil Science Society of America Journal 40, 876–879.
Stimulation of plant growth by humic substances.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaE2sXkt1ehsw%3D%3D&md5=ed4924addb341958959698aed16f7281CAS |

Liu C, Cooper RJ, Bowman DC (1998) Humic acid application affects photosynthesis, root development, and nutrient content of creeping bentgrass. HortScience 33, 1023–1025.

Lodhi A (2013) Characterization of commercial humic acid samples and their impact on growth of fungi and plants. Pakistan Journal of Soil Science 32, 63–70.

MacCarthy P (2001) The principles of humic substances. Soil Science 166, 738–751.
The principles of humic substances.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXovVersbk%3D&md5=abbe53e9d65f54d2183aec117adfc19dCAS |

Meng L, Ding W, Cai Z (2005) Long-term application of organic manure and nitrogen fertilizer on N2O emissions, soil quality and crop production in a sandy loam soil. Soil Biology & Biochemistry 37, 2037–2045.
Long-term application of organic manure and nitrogen fertilizer on N2O emissions, soil quality and crop production in a sandy loam soil.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXhtFKntLnI&md5=a171167e686d0f659dc8b87a410e3f17CAS |

Muscolo A, Cutrupi S, Nardi S (1998) IAA detection in humic substances. Soil Biology & Biochemistry 30, 1199–1201.
IAA detection in humic substances.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1cXktlKms78%3D&md5=b5ca065791d31d4425f9c00463080cdbCAS |

Muscolo A, Sidari M, Nardi S (2013) Humic substance: Relationship between structure and activity. Deeper information suggests univocal findings. Journal of Geochemical Exploration 129, 57–63.

Nardi S, Pizzeghello D, Muscolo A, Vianello A (2002) Physiological effects of humic substances on higher plants. Soil Biology & Biochemistry 34, 1527–1536.
Physiological effects of humic substances on higher plants.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38Xps12ltrY%3D&md5=b880e26a415a12c554bed50fa9a1477cCAS |

Olk D, Dinnes D, Callaway C, Raske M (2013) On-farm evaluation of a humic product in Iowa (US) maize production. In ‘Functions of natural organic matter in changing environment’ . (Eds J Xu, J Wu, Y He) (Springer: Berlin, Heidelberg)

Phillips J, Hayman D (1970) Improved procedures for clearing roots and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection. Transactions of the British Mycological Society 55, 158–161.
Improved procedures for clearing roots and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection.Crossref | GoogleScholarGoogle Scholar |

Piccolo A, Celano G, Pietramellara G (1993) Effects of fractions of coal-derived humic substances on seed germination and growth of seedlings. Biology and Fertility of Soils 16, 11–15.
Effects of fractions of coal-derived humic substances on seed germination and growth of seedlings.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3sXms12rtbw%3D&md5=4d73f01bd4a91a7e6e94d673413f77ffCAS |

Piccolo A, Pietramellara G, Mbagwu JSC (1997) Use of humic substances as soil conditioners to increase aggregate stability. Geoderma 75, 267–277.
Use of humic substances as soil conditioners to increase aggregate stability.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2sXhvFKhsbg%3D&md5=375b1ecd123aeadae68fb76d2e98ace2CAS |

Pilanal N, Kaplan M (2003) Investigation of effects on nutrient uptake of humic acid applications of different forms to strawberry plant. Journal of Plant Nutrition 26, 835–843.
Investigation of effects on nutrient uptake of humic acid applications of different forms to strawberry plant.Crossref | GoogleScholarGoogle Scholar |

Puglisi E, Fragoulis G, Ricciuti P, Cappa F, Spaccini R, Piccolo A, Trevisan M, Crecchio C (2009) Effects of a humic acid and its size-fractions on the bacterial community of soil rhizosphere under maize (Zea mays L.). Chemosphere 77, 829–837.
Effects of a humic acid and its size-fractions on the bacterial community of soil rhizosphere under maize (Zea mays L.).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXht1GntrfO&md5=a78f168e65481260e62d3081d06bcd41CAS | 19712956PubMed |

Quilty JR, Cattle SR (2011) Use and understanding of organic amendments in Australian agriculture: a review. Soil Research 49, 1–26.
Use and understanding of organic amendments in Australian agriculture: a review.Crossref | GoogleScholarGoogle Scholar |

Rose MT, Patti AF, Little KR, Brown AL, Jackson WR, Cavagnaro TR (2014) A meta-analysis and review of plant-growth response to humic substances: Practical implications for agriculture. In ‘Advances in agronomy’ . Ch. 2. (Ed. LS Donald) (Academic Press: Waltham, MA, USA)

Ryan M, Ash J (1999) Effects of phosphorus and nitrogen on growth of pasture plants and VAM fungi in SE Australian soils with contrasting fertiliser histories (conventional and biodynamic). Agriculture, Ecosystems & Environment 73, 51–62.
Effects of phosphorus and nitrogen on growth of pasture plants and VAM fungi in SE Australian soils with contrasting fertiliser histories (conventional and biodynamic).Crossref | GoogleScholarGoogle Scholar |

Schweiger PF, Robson AD, Barrow NJ (1995) Root hair length determines beneficial effect of a Glomus species on shoot growth of some pasture species. New Phytologist 131, 247–254.
Root hair length determines beneficial effect of a Glomus species on shoot growth of some pasture species.Crossref | GoogleScholarGoogle Scholar |

Seyedbagheri M, He Z, Olk D (2012) Yields of potato and alternative crops impacted by humic product application. In ‘Sustainable potato production: Global case studies’ . (Eds Z He, R Larkin, W Honeycutt) (Springer: Berlin, Heidelberg)

Smith SE, Read DJ (2010) ‘Mycorrhizal symbiosis.’ (Elsevier: Amsterdam) http://books.google.com.au (accessed November 2013)

Sparling G (1992) Ratio of microbial biomass carbon to soil organic carbon as a sensitive indicator of changes in soil organic matter. Soil Research 30, 195–207.
Ratio of microbial biomass carbon to soil organic carbon as a sensitive indicator of changes in soil organic matter.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK38XitlCms7g%3D&md5=78c25593f3a9268b38fac33835daa1a2CAS |

Sparling GP, Wheeler D, Vesely E-T, Schipper LA (2006) What is soil organic matter worth? Journal of Environmental Quality 35, 548–557.
What is soil organic matter worth?Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XisFKrurw%3D&md5=616eca35596d8b9b99934e36398d901fCAS | 16510699PubMed |

Tahir MM, Khurshid M, Khan MZ, Abbasi MK, Kazmi MH (2011) Lignite-derived humic acid effect on growth of wheat plants in different soils. Pedosphere 21, 124–131.
Lignite-derived humic acid effect on growth of wheat plants in different soils.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXislegsLc%3D&md5=ac6d35b9b7d815c5ef523b7492bc2c9dCAS |

Tan KH, Nopamornbodi V (1979) Effect of different levels of humic acids on nutrient content and growth of corn (Zea mays L.). Plant and Soil 51, 283–287.
Effect of different levels of humic acids on nutrient content and growth of corn (Zea mays L.).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaE1MXhvVGmtb0%3D&md5=37e0829627794c06f2e37ed0e649d399CAS |

Tilman D, Cassman KG, Matson PA, Naylor R, Polasky S (2002) Agricultural sustainability and intensive production practices. Nature 418, 671–677.
Agricultural sustainability and intensive production practices.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XlvVyltb0%3D&md5=3cb76b85f182e4951c51c221f9594613CAS | 12167873PubMed |

Vallini G, Pera A, Avio L, Valdrighi M, Giovannetti M (1993) Influence of humic acids on laurel growth, associated rhizospheric microorganisms, and mycorrhizal fungi. Biology and Fertility of Soils 16, 1–4.
Influence of humic acids on laurel growth, associated rhizospheric microorganisms, and mycorrhizal fungi.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3sXms12rtb8%3D&md5=fd316ad13123775d8cbbc967dc24aeb5CAS |

Vance ED, Brookes PC, Jenkinson DS (1987) An extraction method for measuring soil microbial biomass C. Soil Biology & Biochemistry 19, 703–707.
An extraction method for measuring soil microbial biomass C.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL1cXjs1KqsA%3D%3D&md5=b6d046fc432a1df6ee725571fdc9b35cCAS |

Verlinden G, Pycke B, Mertens J, Debersaques F, Verheyen K, Baert G, Bries J, Haesaert G (2009) Application of humic substances results in consistent increases in crop yield and nutrient uptake. Journal of Plant Nutrition 32, 1407–1426.
Application of humic substances results in consistent increases in crop yield and nutrient uptake.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXptl2js7o%3D&md5=0e9d29c89e145b116d908f315aa7a88eCAS |

Verlinden G, Coussens T, De Vliegher A, Baert G, Haesaert G (2010) Effect of humic substances on nutrient uptake by herbage and on production and nutritive value of herbage from sown grass pastures. Grass and Forage Science 65, 133–144.
Effect of humic substances on nutrient uptake by herbage and on production and nutritive value of herbage from sown grass pastures.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXktlSnurs%3D&md5=ed004958d0c330eac2f609154fff8661CAS |

Visser SA (1985) Effect of humic acids on numbers and activities of micro-organisms within physiological groups. Organic Geochemistry 8, 81–85.
Effect of humic acids on numbers and activities of micro-organisms within physiological groups.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL2MXksFWjurs%3D&md5=dc932275d10962f7d342825cb24fe22cCAS |

Yolcu H, Seker H, Gullap MK, Lithourgidis A, Gunes A (2011) Application of cattle manure, zeolite and leonardite improves hay yield and quality of annual ryegrass (Lolium multiflorum Lam.) under semiarid conditions. Australian Journal of Crop Science 5, 926–931.