QTL dissection of yield components and morpho-physiological traits in a durum wheat elite population tested in contrasting thermo-pluviometric conditions
M. Graziani A , M. Maccaferri A , C. Royo B , F. Salvatorelli A and R. Tuberosa A C
+ Author Affiliations
- Author Affiliations
A DipSA, Department of Agricultural Science, Viale Giuseppe Fanin 44, 40127 Bologna, Italy.
B IRTA (Institute for Food and Agricultural Research and Technology), Field Crops Section, 25198 Lleida, Spain.
C Corresponding author. Email: roberto.tuberosa@unibo.it.
Crop and Pasture Science 65(1) 80-95 https://doi.org/10.1071/CP13349
Submitted: 14 October 2013 Accepted: 23 December 2013 Published: 30 January 2014
Abstract
Dissection of the genetic basis of the adaptive response of durum wheat to unfavourable water and temperature regimes is an important prerequisite for the selection of genotypes less vulnerable to environmental constraints. An elite durum population of 249 recombinant inbred lines was tested across 16 Mediterranean environments characterised by contrasting thermo-pluviometric conditions and, consequently, a broad range of productivity (from 0.56 to 5.88 t ha–1). Among the environmental variables investigated, soil moisture during grain filling showed the most consistent correlation with yield components and grain yield, whereas a weaker, albeit in some cases significant, association was noted with temperature at heading and thermal time during grain filling. Ear peduncle length appeared as a valid and easy-to-phenotype morphological proxy for the water available to the plant. In total, 76 quantitative trait loci (QTLs) were identified for yield components and for several morpho-physiological traits (peduncle length, the spectral reflectance index NDVI and leaf greenness at the milk-grain stage expressed in SPAD units) associated with the adaptive response of wheat to water and heat stresses. Although most of the QTLs were significant in only one or two environments, two major QTLs on chromosomes 2BL and 3BS showed consistent additive and epistatic effects on 1000-kernel weight, peduncle length, SPAD values and grain yield in half of the environments. In view of their strong phenotypic effects on kernel weight, these two QTLs are good candidates for positional cloning in order to gain a better understanding of the functional basis of their effect on the plasticity of grain weight and grain yield.
Additional keywords: drought, durum wheat, heat stress, QTL, QTL mapping, Triticum, wheat quality.
References
Aisawi K, Foukes J, Reynolds M, Mayes S (2010) The physiological basis of genetic progress in yield potential of CIMMYT wheat varieties from 1966 to 2009. In ‘Abstracts 8th International Wheat Conference’. 1–4 June 2010. St Petersburg, Russia. pp. 349–350. (N I Vavilov Research Institute: St Petersburg, Russia)Alvaro F, Isidro J, Villegas D, García del Moral LF, Royo C (2008a) Breeding effects on grain filling, biomass partitioning, and remobilization in Mediterranean durum wheat. Agronomy Journal 100, 361–370.
| Breeding effects on grain filling, biomass partitioning, and remobilization in Mediterranean durum wheat.Crossref | GoogleScholarGoogle Scholar |
Alvaro F, Royo C, García del Moral LF, Villegas D (2008b) Grain filling and dry matter translocation responses to source-sink modifications in a historical series of durum wheat. Crop Science 48, 1523–1531.
| Grain filling and dry matter translocation responses to source-sink modifications in a historical series of durum wheat.Crossref | GoogleScholarGoogle Scholar |
Aparicio N, Villegas D, Casadesús J, Araus JL, Royo C (2000) Spectral vegetation indices as nondestructive tools for determining durum wheat yield. Agronomy Journal 92, 83–91.
| Spectral vegetation indices as nondestructive tools for determining durum wheat yield.Crossref | GoogleScholarGoogle Scholar |
Araus JL, Bort J, Steduto P, Villegas D, Royo C (2003a) Breeding cereals for Mediterranean conditions: ecophysiological clues for biotechnology application. Annals of Applied Biology 142, 129–141.
| Breeding cereals for Mediterranean conditions: ecophysiological clues for biotechnology application.Crossref | GoogleScholarGoogle Scholar |
Araus JL, Villegas D, Aparicio N, García del Moral LF, ElHani S, Rharrabti Y, Ferrio JP, Royo C (2003b) Environmental factors determining carbon isotope discrimination and yield in durum wheat under Mediterranean conditions. Crop Science 43, 170–180.
| Environmental factors determining carbon isotope discrimination and yield in durum wheat under Mediterranean conditions.Crossref | GoogleScholarGoogle Scholar |
Bennett D, Izanloo A, Reynolds M, Kuchel H, Langridge P, Schnurbusch T (2012a) Genetic dissection of grain yield and physical grain quality in bread wheat (Triticum aestivum L.) under water-limited environments. Theoretical and Applied Genetics 125, 255–271.
| Genetic dissection of grain yield and physical grain quality in bread wheat (Triticum aestivum L.) under water-limited environments.Crossref | GoogleScholarGoogle Scholar | 22374139PubMed |
Bennett D, Reynolds M, Mullan D, Izanloo A, Kuchel H, Langridge P, Schnurbusch T (2012b) Detection of two major grain yield QTL in bread wheat (Triticum aestivum L.) under heat, drought and high yield potential environments. Theoretical and Applied Genetics 125, 1473–1485.
| Detection of two major grain yield QTL in bread wheat (Triticum aestivum L.) under heat, drought and high yield potential environments.Crossref | GoogleScholarGoogle Scholar | 22772727PubMed |
Blanco A, Mangini G, Giancaspro A, Giove S, Colasuonno P, Simeone R, Signorile A, De Vita P, Mastrangelo AM, Cattivelli L, Gadaleta A (2012) Relationships between grain protein content and grain yield components through quantitative trait locus analyses in a recombinant inbred line population derived from two elite durum wheat cultivars. Molecular Breeding 30, 79–92.
| Relationships between grain protein content and grain yield components through quantitative trait locus analyses in a recombinant inbred line population derived from two elite durum wheat cultivars.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XnvFOlurk%3D&md5=557c1f530ed7aeb5692abc4c0d427498CAS |
Blum A (1998) Improving wheat grain filling under stress by stem reserve mobilisation. Euphytica 100, 77–83.
| Improving wheat grain filling under stress by stem reserve mobilisation.Crossref | GoogleScholarGoogle Scholar |
Blum A (2011) Plant water relations, plant stress and plant production. In ‘Plant breeding for water-limited environments’. (Ed. A Blum) pp. 11–52. (Springer: New York)
Bonneau J, Taylor J, Parent B, Bennett D, Reynolds M, Feuillet C, Langridge P, Mather D (2013) Multi-environment analysis and improved mapping of a yield-related QTL on chromosome 3B of wheat. Theoretical and Applied Genetics 126, 747–761.
| Multi-environment analysis and improved mapping of a yield-related QTL on chromosome 3B of wheat.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXjtlWrsrg%3D&md5=5f7e32c5dc266a79eff6daadb9b4d82cCAS | 23262551PubMed |
Breseghello F, Sorrells ME (2006) Association mapping of kernel size and milling quality in wheat (Triticum aestivum L.) cultivars. Genetics 172, 1165–1177.
| Association mapping of kernel size and milling quality in wheat (Triticum aestivum L.) cultivars.Crossref | GoogleScholarGoogle Scholar | 16079235PubMed |
Brouder SM, Volenec JJ (2008) Impact of climate change on crop nutrient and water use efficiencies. Physiologia Plantarum 133, 705–724.
| Impact of climate change on crop nutrient and water use efficiencies.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXps1Oit7k%3D&md5=c1ed05268c57e5ed1ab1fd132819944fCAS | 18507815PubMed |
Collins NC, Tardieu F, Tuberosa R (2008) Quantitative trait loci and crop performance under abiotic stress: where do we stand? Plant Physiology 147, 469–486.
| Quantitative trait loci and crop performance under abiotic stress: where do we stand?Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXnsVyhsbw%3D&md5=48d814a451b7e894ee249543fe918223CAS | 18524878PubMed |
Conti V, Roncallo PF, Beaufort V, Cervigni GL, Miranda R, Jensen CA, Echenique VC (2011) Mapping of main and epistatic effect QTLs associated to grain protein and gluten strength using a RIL population of durum wheat. Journal of Applied Genetics 52, 287–298.
| Mapping of main and epistatic effect QTLs associated to grain protein and gluten strength using a RIL population of durum wheat.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXhtlSqt7nJ&md5=83ff327c587d7aaca8e8f9e98b358f72CAS | 21523429PubMed |
Cuthbert JL, Somers DJ, Brûlé-Babel AL, Brown PD, Crow GH (2008) Molecular mapping of quantitative trait loci for yield and yield components in spring wheat (Triticum aestivum L.). Theoretical and Applied Genetics 117, 595–608.
| Molecular mapping of quantitative trait loci for yield and yield components in spring wheat (Triticum aestivum L.).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXovFOntr4%3D&md5=dcf486fc68f9383f1b552a3d54893d50CAS | 18516583PubMed |
Darvasi A, Soller M (1997) A simple method to calculate resolving power and confidence interval of QTL map location. Behavior Genetics 27, 125–132.
| A simple method to calculate resolving power and confidence interval of QTL map location.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaK2s3ptV2htw%3D%3D&md5=abf89d3008185d0ff59f0feaa0c62b6aCAS | 9145551PubMed |
Diab AA, Kantety RV, Ozturk NZ, Benscher D, Nachit MM, Sorrells ME (2008) Drought-inducible genes and differentially expressed sequence tags associated with components of drought tolerance in durum wheat. Scientific Research and Essays 3, 9–27.
Fisher RA (2011) Wheat physiology: a review of recent developments. Crop & Pasture Science 62, 95–114.
Frederick JR, Bauer PJ (1999) Physiological and numerical components of wheat yield. In ‘Wheat: ecology and physiology of yield determination’. (Eds EH Satorre, GA Slafer) pp. 45–65. (Food Product Press: New York)
Garcia del Moral LF, Rharrabti Y, Villegas D, Royo C (2003) Evaluation of grain yield and its components in durum wheat under Mediterranean conditions: an ontogenic approach. Agronomy Journal 95, 266–274.
| Evaluation of grain yield and its components in durum wheat under Mediterranean conditions: an ontogenic approach.Crossref | GoogleScholarGoogle Scholar |
Gregersen PL, Culetic A, Boschian L, Krupinska K (2013) Plant senescence and crop productivity. Plant Molecular Biology 82, 603–622.
| Plant senescence and crop productivity.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXhtFOkt77J&md5=56848bda0bfb909342b22d4233c0bd55CAS | 23354836PubMed |
Kao CH, Zeng ZB, Teasdale RD (1999) Multiple interval mapping for quantitative trait loci. Genetics 152, 1203–1216.
Kong L, Wang F, Feng B, Li S, Si J, Zhang B (2010) The structural and photosynthetic characteristics of the exposed peduncle of wheat (Triticum aestivum L.): an important photosynthate source for grain-filling. BMC Plant Biology 10, 141
| The structural and photosynthetic characteristics of the exposed peduncle of wheat (Triticum aestivum L.): an important photosynthate source for grain-filling.Crossref | GoogleScholarGoogle Scholar | 20618993PubMed |
Kroymann J, Mitchell-Olds T (2005) Epistasis and balanced polymorphism influencing complex trait variation. Nature 435, 95–98.
| Epistasis and balanced polymorphism influencing complex trait variation.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXjvVKntbs%3D&md5=b37f588a40645a87fa50d54f67e609aaCAS | 15875023PubMed |
Kuchel H, Williams K, Langridge P, Eagles HA, Jefferies SP (2007) Genetic dissection of grain yield in bread wheat. II. QTL-by-environment interaction. Theoretical and Applied Genetics 115, 1015–1027.
| Genetic dissection of grain yield in bread wheat. II. QTL-by-environment interaction.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD2sngtFOjsQ%3D%3D&md5=8db9999440d1ac07261cb274d0a61898CAS | 17712541PubMed |
Maccaferri M, Sanguineti MC, Corneti S, Ortega JLA, Ben Salem M, Bort J, DeAmbrogio E, Del Moral LFG, Demontis A, El-Ahmed A, Maalouf F, Machlab H, Martos V, Moragues M, Motawaj J, Nachit M, Nserallah N, Ouabbou H, Royo C, Slama A, Tuberosa R (2008a) Quantitative trait loci for grain yield and adaptation of durum wheat (Triticum durum Desf.) across a wide range of water availability. Genetics 178, 489–511.
| Quantitative trait loci for grain yield and adaptation of durum wheat (Triticum durum Desf.) across a wide range of water availability.Crossref | GoogleScholarGoogle Scholar | 18202390PubMed |
Maccaferri M, Sanguineti MC, Giuliani S, Tuberosa R (2008b). Genomics of tolerance to abiotic stress in the Triticeae. In ‘Triticeae genomics’. (Eds C Feuillet, G Muhelbauer) pp. 259–318. (Springer: Dordrecht, the Netherlands)
Maccaferri M, Sanguineti MC, Demontis A, El-Ahmed A, Garcia del Moral L, Maalouf F, Nachit M, Nserallah N, Ouabbou H, Rhouma S, Royo C, Villegas D, Tuberosa R (2011) Association mapping in durum wheat grown across a broad range of water regimes. Journal of Experimental Botany 62, 409–438.
| Association mapping in durum wheat grown across a broad range of water regimes.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXhsFyrsb3N&md5=4ae26599cb0d983556e0d0c94f4ec51aCAS | 21041372PubMed |
Marti J, Bort J, Slafer GA, Araus JL (2007) Can wheat yield be assessed by early measurements of Normalized Difference Vegetation Index? Annals of Applied Biology 150, 253–257.
| Can wheat yield be assessed by early measurements of Normalized Difference Vegetation Index?Crossref | GoogleScholarGoogle Scholar |
Mathews KL, Malosetti M, Chapman S, McIntyre L, Reynolds M, Shorter R, Van Eeuwijk F (2008) Multi-environment QTL mixed models for drought stress adaptation in wheat. Theoretical and Applied Genetics 117, 1077–1091.
| Multi-environment QTL mixed models for drought stress adaptation in wheat.Crossref | GoogleScholarGoogle Scholar | 18696042PubMed |
McIntyre CL, Mathews KL, Rattey A, Chapman SC, Drenth J, Ghaderi M, Reynolds M, Shorter R (2010) Molecular detection of genomic regions associated with grain yield and yield-related components in an elite bread wheat cross evaluated under irrigated and rainfed conditions. Theoretical and Applied Genetics 120, 527–541.
| Molecular detection of genomic regions associated with grain yield and yield-related components in an elite bread wheat cross evaluated under irrigated and rainfed conditions.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXotV2iug%3D%3D&md5=75229ec1a26de197ad3b127b77f5930eCAS | 19865806PubMed |
Paux E, Legeai F, Guilhot N, Adam-Blondon A-F, Alaux M, Salse J, Sourdille P, Leroy P, Feuillet C (2008) Physical mapping in large genomes: accelerating anchoring of BAC contigs to genetic maps through in silico analysis. Functional & Integrative Genomics 8, 29–32.
| Physical mapping in large genomes: accelerating anchoring of BAC contigs to genetic maps through in silico analysis.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXhsVGhur3E&md5=0b2c2658e9451780f171276a68e43a86CAS |
Pinto RS, Reynolds MP, Mathews KL, McIntyre CL, Olivares-Villegas J-J, Chapman SC (2010) Heat and drought adaptive QTL in a wheat population designed to minimize confounding agronomic effects. Theoretical and Applied Genetics 121, 1001–1021.
| Heat and drought adaptive QTL in a wheat population designed to minimize confounding agronomic effects.Crossref | GoogleScholarGoogle Scholar | 20523964PubMed |
Quarrie S, Pekic Quarrie S, Radosevic R, Rancic D, Kaminska A, Barnes JD, Leverington M, Ceoloni C, Dodig D (2006) Dissecting a wheat QTL for yield present in a range of environments: from the QTL to candidate genes. Journal of Experimental Botany 57, 2627–2637.
| Dissecting a wheat QTL for yield present in a range of environments: from the QTL to candidate genes.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28Xotl2mt7Y%3D&md5=fd146636e011a17519cc87e3dd30ffb0CAS | 16831847PubMed |
Rebetzke GJ, Condon AG, Farquhar GD, Appels R, Richards RA (2008) Quantitative trait loci for carbon isotope discrimination are repeatable across environments and wheat mapping populations. Theoretical and Applied Genetics 118, 123–137.
| Quantitative trait loci for carbon isotope discrimination are repeatable across environments and wheat mapping populations.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD1cjivFKjsg%3D%3D&md5=392373972641fa08f07ac6348ffff2aeCAS | 18818897PubMed |
Reynolds M, Tuberosa R (2008) Translational research impacting on crop productivity in drought-prone environments. Current Opinion in Plant Biology 11, 171–179.
| Translational research impacting on crop productivity in drought-prone environments.Crossref | GoogleScholarGoogle Scholar | 18329330PubMed |
Royo C, Álvaro F, Martos V, Ramdani A, Isidro J, Villegas D, del Moral LFG (2007) Genetic changes in durum wheat yield components and associated traits in Italian and Spanish varieties during the 20th century. Euphytica 155, 259–270.
| Genetic changes in durum wheat yield components and associated traits in Italian and Spanish varieties during the 20th century.Crossref | GoogleScholarGoogle Scholar |
Royo C, Maccaferri M, Álvaro F, Moragues M, Sanguineti MC, Tuberosa R, Maalouf F, del Moral LFG, Demontis A, Rhouma S, Nachit M, Nserallah N, Villegas D (2010) Understanding the relationships between genetic and phenotypic structures of a collection of elite durum wheat accessions. Field Crops Research 119, 91–105.
| Understanding the relationships between genetic and phenotypic structures of a collection of elite durum wheat accessions.Crossref | GoogleScholarGoogle Scholar |
Sanchez-Garcia M, Royo C, Aparicio N, Martín-Sánchez JA, Alvaro F (2013) Genetic improvement of bread wheat yield and associated traits in Spain during the 20th century. The Journal of Agricultural Science 151, 105–118.
| Genetic improvement of bread wheat yield and associated traits in Spain during the 20th century.Crossref | GoogleScholarGoogle Scholar | 23365469PubMed |
Tardieu F (2012) Any trait or trait-related allele can confer drought tolerance: just design the right drought scenario. Journal of Experimental Botany 63, 25–31.
| Any trait or trait-related allele can confer drought tolerance: just design the right drought scenario.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXhs1yms73L&md5=2b1e13e95d7cfd94160acf2c622b2d2aCAS | 21963615PubMed |
Tardieu F, Tuberosa R (2010) Dissection and modelling of abiotic stress tolerance in plants. Current Opinion in Plant Biology 13, 206–212.
| Dissection and modelling of abiotic stress tolerance in plants.Crossref | GoogleScholarGoogle Scholar | 20097596PubMed |
Trebbi D, Maccaferri M, De Heer P, Sørensen A, Giuliani S, Salvi S, Sanguineti MC, Massi A, Van der Vossen EAG, Tuberosa R (2011) High-throughput SNP discovery and genotyping in durum wheat (Triticum durum Desf.). Theoretical and Applied Genetics 123, 555–569.
| High-throughput SNP discovery and genotyping in durum wheat (Triticum durum Desf.).Crossref | GoogleScholarGoogle Scholar | 21611761PubMed |
Tuberosa R (2012) Phenotyping for drought tolerance of crops in the genomics era. Frontiers in Physiology 3, 1–26.
| Phenotyping for drought tolerance of crops in the genomics era.Crossref | GoogleScholarGoogle Scholar |
van Poecke RMP, Maccaferri M, Tang J, Truong HT, Janssen A, Van Orsouw NJ, Salvi S, Sanguineti MC, Tuberosa R, Van der Vossen EG (2013) Sequence-based SNP genotyping in durum wheat. Plant Biotechnology Journal 11, 809–817.
| Sequence-based SNP genotyping in durum wheat.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXhsFKlur7L&md5=0829f3746cd5a49511b0753d020cfbd7CAS |
Varshney RK, Prasad M, Roy JK, Harjit-Singh NK, Dhaliwal HS, Balyan HS, Gupta PK (2000) Identification of eight chromosomes and a microsatellite marker on 1AS associated with QTL for grain weight in bread wheat. Theoretical and Applied Genetics 100, 1290–1294.
| Identification of eight chromosomes and a microsatellite marker on 1AS associated with QTL for grain weight in bread wheat.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXlsF2gtrw%3D&md5=0d60053c3252eb263fc4e3ce96f79a19CAS |
Zadoks JC, Chang TT, Konzak CF (1974) A decimal code for the growth stages of cereals. Weed Research 14, 415–421.
Zarei L, Cheghamirza K, Farshadfar E (2013) Evaluation of grain yield and some agronomic characters in durum wheat (Triticum turgidum L.) under rainfed conditions. Australian Journal of Crop Science 7, 609–617.
Zhao X, Qin Y, Jia B, Kim S-M, Lee H-S, Eun M-Y, Kim K-M, Sohn J-K (2013) Comparison and analysis of QTLs, epistatic effects and QTL × environment interactions for yield traits using DH and RILs populations in rice. Journal of Integrative Agriculture 12, 198–208.
| Comparison and analysis of QTLs, epistatic effects and QTL × environment interactions for yield traits using DH and RILs populations in rice.Crossref | GoogleScholarGoogle Scholar |
