Fast identification of wheat 1BL.1RS translocation by reversed-phase ultra-performance liquid chromatography (RP-UPLC)
Jianwen Zhou A C , Caixia Han A C , Hui Cao A , Shoumin Zhen A , Zitong Yu A , Xiaohui Li A , Wujun Ma B and Yueming Yan A D
+ Author Affiliations
- Author Affiliations
A College of Life Science, Capital Normal University, Beijing 100048, China.
B Centre for comparative Genomics, Murdoch University and Australian Export Grain Innovation Centre, Perth, WA 6150, Australia.
C These authors contributed equally to this work.
D Corresponding author. Email: yanym@cnu.edu.cn
Crop and Pasture Science 64(9) 865-873 https://doi.org/10.1071/CP13246
Submitted: 10 July 2013 Accepted: 12 September 2013 Published: 6 November 2013
Journal Compilation © CSIRO Publishing 2013 Open Access CC BY-NC-ND
Abstract
The 1BL.1RS chromosomal translocation in wheat is the result of replacement of the short arm of chromosome 1B of wheat by the short arm of chromosome 1R of rye, which had been widely used as a parental line in worldwide wheat breeding, resulting in a high percentage of wheat cultivars containing this translocation. A fast and reliable approach to identify this translocation is highly desirable in modern wheat breeding. This study compared reversed-phase ultra-performance liquid chromatography (RP-UPLC), acidic polyacrylamide gel electrophoresis (A-PAGE), liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS), allelic-specific PCR, and reversed-phase high-performance liquid chromatography (RP-HPLC) approaches to identify the 1BL.1RS translocation in 76 bread wheat cultivars. Two gliadin bands in the Gli-B1 region of A-PAGE separation were confirmed by LC-MS/MS to be omega secalins from the 1BL.1RS translocation, and they can be used as reliable protein markers for identifying the translocation. A few specific minor peaks eluted at 12–13 min on the RP-UPLC patterns can readily differentiate the 1BL.1RS translocation. Of the 76 wheat cultivars tested, 40 were identified as carrying the 1BL.1RS translocation by RP-UPLC, which was consistent with the results of A-PAGE, HPLC, and PCR. Compared with other established methods, RP-UPLC showed a clear advantage in fast identification of the 1BL.1RS translocation with higher reliability and lower costs, and it is therefore ideal for large-scale screening of the 1BL.1RS translocation in wheat breeding.
Additional keywords: wheat, 1BL.1RS translocation, A-PAGE, HPLC, PCR, UPLC.
References
Bagge M, Xia XC, Lubberstedt T (2007) Functional markers in wheat. Current Opinion in Plant Biology 10, 211–216.| Functional markers in wheat.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXitl2gsrc%3D&md5=5e11fc8f3b1f6586ad5a0f7565fc7081CAS | 17292659PubMed |
Baum M, Appels R (1991) The cytogenetics and molecular architecture of chromosome 1R—one of the most widely utilized source of alien chromatin in wheat varieties. Chromosoma 101, 1–10.
| The cytogenetics and molecular architecture of chromosome 1R—one of the most widely utilized source of alien chromatin in wheat varieties.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2MXhsVOjtQ%3D%3D&md5=14e670f8fa3e49592b487ecda9035023CAS | 1769268PubMed |
Bennett MD, Smith JB (1975) Confirmation of the identification of the rye chromosome in 1B.1R wheat-rye chromosome substitution and translocation lines. Canadian Journal of Genetics and Cytology 17, 117–120.
Bietz JA (1983) Separation of cereal proteins by reversed-phase high-performance liquid chromatography. Journal of Chromatography. A 255, 219–238.
| Separation of cereal proteins by reversed-phase high-performance liquid chromatography.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL3sXmvVagtA%3D%3D&md5=48dee081ac9fb0b3124b438852ac5cb7CAS |
Chai JF, Zhou RH, Jia JZ, Liu X (2006) Development and application of a new codominant PCR marker for detecting 1BL·1RS wheat–rye chromosome translocations. Plant Breeding 125, 302–304.
| Development and application of a new codominant PCR marker for detecting 1BL·1RS wheat–rye chromosome translocations.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XntlWqsr0%3D&md5=cca3388016b1a107c82b2e7c1f5fe90fCAS |
Chojecki AJS, Gale MD, Holt LM, Payne PI (1983) The intrachromosomal mapping of a glucose phosphate isomerase structural gene, using allelic variation among stocks of Chinese Spring wheat. Genetical Research 41, 221–226.
| The intrachromosomal mapping of a glucose phosphate isomerase structural gene, using allelic variation among stocks of Chinese Spring wheat.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL3sXmtV2qtL0%3D&md5=489efefba5861b43c2949b7360cfeb50CAS |
Clarke BC, Mukai Y, Appels R (1996) The Sec-1 locus on the short arm of chromosome 1R of rye (Secale cereale). Chromosoma 105, 269–275.
De Vita P, Ficco DBM, Luciani A, Vincentini O, Pettoello-Mantovani M, Silano M, Maiuri L, Cattivelli L (2012) A-secalin contained decamer shows a celiac disease prevention activity. Journal of Cereal Science 55, 234–242.
| A-secalin contained decamer shows a celiac disease prevention activity.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38Xis1antbY%3D&md5=8a0fdd19013681831d3825ca63add8cfCAS |
Dhaliwal AS, Mares DJ, Skeritt JH, Marshall DR (1988) Protein composition and pentosan content in relation to dough stickiness of 1B/1R translocation wheats. Cereal Chemistry 62, 143–149.
Dong K, Hao CY, Wang AL, Cai MH, Yan YM (2009) Characterization of HMW glutenin subunits in bread and tetraploid wheats by reversed-phase high-performance liquid chromatography. Cereal Research Communications 37, 65–73.
| Characterization of HMW glutenin subunits in bread and tetraploid wheats by reversed-phase high-performance liquid chromatography.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXkvVGqsbg%3D&md5=219099fcabbc5caba2e363c2ff6a2a4eCAS |
Friebe B, Heun M, Bushuk W (1989) Cytological characterization, powdery mildew resistance and storage protein composition of tetraploid and hexaploid 1BL/IRS wheat-rye translocation lines. Theoretical and Applied Genetics 78, 425–432.
| Cytological characterization, powdery mildew resistance and storage protein composition of tetraploid and hexaploid 1BL/IRS wheat-rye translocation lines.Crossref | GoogleScholarGoogle Scholar |
Gao L, Ma W, Chen J, Wang K, Li J, Wang S, Bekes F, Apples R, Yan Y (2010) Characterization and comparative analysis of wheat high molecular weight glutenin subunits by SDS-PAGE, RP-HPLC, HPCE, and MALDI-TOF-MS. Journal of Agricultural and Food Chemistry 58, 2777–2786.
| Characterization and comparative analysis of wheat high molecular weight glutenin subunits by SDS-PAGE, RP-HPLC, HPCE, and MALDI-TOF-MS.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXhslShsb8%3D&md5=57ae2587690c40b3db771161ae724f1eCAS | 20146422PubMed |
Gobaa S, Bancle E, Kleiger G, Stamp P, Branlard G (2007) Effect of the 1BL.1RS translocation on the wheat endosperm, as revealed by proteomic analysis. Proteomics 7, 4349–4357.
| Effect of the 1BL.1RS translocation on the wheat endosperm, as revealed by proteomic analysis.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXhsVOqsL%2FP&md5=94658882ae366b9116ef0e16caed0709CAS | 17973293PubMed |
Graybosch RA (2001) Uneasy unions: quality effects of rye chromatin transfers to wheat. Journal of Cereal Science 33, 3–16.
| Uneasy unions: quality effects of rye chromatin transfers to wheat.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXlt1Oksw%3D%3D&md5=b5984885621f79a0a752544c08ddb906CAS |
Gustafson JP, Dera AR, Petrovic S (1988) Expression of modified rye ribosomal RNA genes in wheat. Proceedings of the National Academy of Sciences of the United States of America 85, 3943–3945.
| Expression of modified rye ribosomal RNA genes in wheat.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC3cnivFGmtw%3D%3D&md5=696bfd7dcff256bd65266045bb8e7fccCAS | 16593938PubMed |
Heslop-Harrison JS, Leitch AR, Schwarzacher T, Anamthawat-Jónsson K (1990) Detection and characterization of 1B/1R translocations in hexaploid wheat. Heredity 65, 385–392.
| Detection and characterization of 1B/1R translocations in hexaploid wheat.Crossref | GoogleScholarGoogle Scholar |
Heun M, Fischbeck G (1987) Identification of wheat powdery mildew resistance genes by analysing host-pathogen interactions. Plant Breeding 98, 124–129.
| Identification of wheat powdery mildew resistance genes by analysing host-pathogen interactions.Crossref | GoogleScholarGoogle Scholar |
Howes NK, Lukow OM, Dawood MR, Bushuk W (1989) Rapid detection of the 1BL.1RS chromosome translocation in hexaploid wheats by monoclonal antibodies. Journal of Cereal Science 10, 1–4.
| Rapid detection of the 1BL.1RS chromosome translocation in hexaploid wheats by monoclonal antibodies.Crossref | GoogleScholarGoogle Scholar |
Javornik B, Sikovié T, Vapa L, Koebner RMD, Rogers WJ (1991) A comparison of methods for identifying and surveying the presence of 1BL.1RS translocations in bread wheat. Euphytica 54, 45–53.
| A comparison of methods for identifying and surveying the presence of 1BL.1RS translocations in bread wheat.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3MXmt1ymsLY%3D&md5=bab2b090557b05cb20a6628980204f15CAS |
Koebner RMD, Shepherd KW (1986) Controlled introgression to wheat of genes from rye chromosome arm IRS by induction of allosyndesis. Theoretical and Applied Genetics 73, 197–208.
| Controlled introgression to wheat of genes from rye chromosome arm IRS by induction of allosyndesis.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL2sXotFOjtQ%3D%3D&md5=864c556ba8fd7f5a2484b3bfd1b3d53cCAS |
Kőszegi B, Linc G, Juhasz L, Lang L, Molnar-Lang M (2000) Occurrence of the 1RS/1BL wheat-rye translocation in hungarian wheat varieties. Acta Agronomica Hungarica 48, 227–236.
| Occurrence of the 1RS/1BL wheat-rye translocation in hungarian wheat varieties.Crossref | GoogleScholarGoogle Scholar |
Landjeva S, Korzun V, Tsanev V, Vladova R, Ganeva G (2006) Distribution of the wheat-rye translocation 1RS.1BL among bread wheat varieties of Bulgaria. Plant Breeding 125, 102–104.
| Distribution of the wheat-rye translocation 1RS.1BL among bread wheat varieties of Bulgaria.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XjsFOksL4%3D&md5=3caccf57192d21c434ddddf5854852f4CAS |
Lelley T, Eder C, Grausgruber H (2004) Influence of 1BL.1RS wheat-rye chromosome translocation on genotype by environment interaction. Journal of Cereal Science 39, 313–320.
| Influence of 1BL.1RS wheat-rye chromosome translocation on genotype by environment interaction.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXksVantLw%3D&md5=f52d2c67d4d8f6ed11073a4056058665CAS |
Lookhart GL, Bean SR (1995a) A fast method for wheat cultivar differentiation using capillary zone electrophoresis. Cereal Chemistry 72, 42–47.
Lookhart GL, Bean SR (1995b) Rapid differentiation of rice cultivars and of oat cultivars by capillary zone electrophoresis. Cereal Chemistry 72, 312–316.
Lookhart GL, Graybosch R, Peterson J, Lukaszewski A (1991) Identification of wheat lines containing the 1BL/1RS translocation by high performance liquid chromatography. Cereal Chemistry 68, 312–316.
Lookhart GL, Bean SR, Graybosch R, Chung OK, Morean-Sevilla B, Baenziger S (1996) Identification by high-performance capillary electrophoresis of wheat lines containing the 1AL.1RS and the 1BL.1RS translocation. Cereal Chemistry 73, 547–550.
Lukaszewski AJ (1990) Frequency of 1RS.1AL and 1RS.1BL translocations in United States wheats. Crop Science 30, 1151–1153.
| Frequency of 1RS.1AL and 1RS.1BL translocations in United States wheats.Crossref | GoogleScholarGoogle Scholar |
Lukaszewski AJ, Gustafson JP (1983) Translocations and modifications of chromosomes in triticale × wheat hybrids. Theoretical and Applied Genetics 64, 239–248.
| Translocations and modifications of chromosomes in triticale × wheat hybrids.Crossref | GoogleScholarGoogle Scholar |
Mettin D, Bliithner WD, Schlegel G (1973) Additional evidence on spontaneous 1B/1R wheat-rye substitutions and translocations. In ‘Proceedings 4th International Wheat Genetics Symposium’. Columbia, Record Number 19751625247. (Eds ER Sears, LMS Sears) pp. 179–184. (Agricultural Experiment Station, College of Agriculture, University of Missouri: Columbia, MO)
Milovanovic M, Perovic D, Saric M, Prodanovic S, Pavlovic K, Jestrovic Z, Protic R (1998) The influence of 1BL.1RS translocation on technological quality of winter wheat. Cereal Research Communications 26, 321–328.
Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research 8, 4321–4325.
Rabinovich SV (1998) Importance of wheat-rye translocations for breeding modern cultivars of Triticum aestivum L. Euphytica 100, 323–340.
| Importance of wheat-rye translocations for breeding modern cultivars of Triticum aestivum L.Crossref | GoogleScholarGoogle Scholar |
Rayburn AL, Carver BF (1988) Cytological identification of 1B/1R wheat-rye translocation in winter wheat breeding lines. Euphytica 38, 237–240.
| Cytological identification of 1B/1R wheat-rye translocation in winter wheat breeding lines.Crossref | GoogleScholarGoogle Scholar |
Shewry PR, Bradberry D, Franklin J, White RP (1984) The chromosomal locations linkage relationships of the structural genes for the prolamin storage proteins (secalins) of rye. Theoretical and Applied Genetics 69, 36–69.
| The chromosomal locations linkage relationships of the structural genes for the prolamin storage proteins (secalins) of rye.Crossref | GoogleScholarGoogle Scholar |
Sozinov AA, Novosel’skaya AY, Lushnikova AA, Bogdanov YF (1987) Cytological and biochemical analysis of bread wheat varieties with 1B/1R substitutions and translocation in the karyotype. Tsitologiia i Genetika 21, 256–261.
Swartz ME (2005) UPLC: An introduction and review. Journal of Liquid Chromatography & Related Technologies 28, 1253–1263.
| UPLC: An introduction and review.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXjsF2lsr8%3D&md5=98ad515da204c0271fb9cdd9d88902d4CAS |
Villareal RL, Bañuelos O, Mujeed-Kazi A (1997) Agronomic performance of related durum wheat (Triticum turgidum L.) stocks possessing the chromosome substitution T1BL.1RS. Crop Science 37, 1735–1740.
| Agronomic performance of related durum wheat (Triticum turgidum L.) stocks possessing the chromosome substitution T1BL.1RS.Crossref | GoogleScholarGoogle Scholar |
Wang SL, Chen D, Guo G, Zhang T, Jiang S, Shen X, Perovic D, Prodanovic S, Yan Y (2012a) Molecular characterization of LMW-GS genes from C, N, U and Ss genomes among Aegilops species. Cereal Research Communications 40, 542–551.
| Molecular characterization of LMW-GS genes from C, N, U and Ss genomes among Aegilops species.Crossref | GoogleScholarGoogle Scholar |
Wang SL, Shen XX, Ge P, Li J, Saminathan S, Li XH, Yan YM (2012b) Molecular characterization and dynamic expression patterns of two types of γ-gliadin genes from Aegilops and Triticum species. Theoretical and Applied Genetics 125, 1371–1384.
| Molecular characterization and dynamic expression patterns of two types of γ-gliadin genes from Aegilops and Triticum species.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XhsFWisbnM&md5=d63969db5c6b2ae49e34e498d7cec52aCAS |
Woychik JH, Boundy JA, Dimler RJ (1961) Starch gel electrophoresis of wheat gluten proteins with concentrated urea. Archives of Biochemistry and Biophysics 94, 477–482.
| Starch gel electrophoresis of wheat gluten proteins with concentrated urea.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaF38XhvFymtA%3D%3D&md5=8d7c399bdcdc836598d626ac1c2cde95CAS | 13786728PubMed |
Wu Y, John R, William E, Hobbins WB (2006) Ultra performance liquid chromatography (UPLC) further improves hydrogen/deuterium exchange mass spectrometry. Journal of the American Society for Mass Spectrometry 17, 163–167.
| Ultra performance liquid chromatography (UPLC) further improves hydrogen/deuterium exchange mass spectrometry.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XhtFyitbY%3D&md5=f97bb23d3765ae764a578bfc99ff482cCAS | 16406808PubMed |
Yan YM, Hsam SLK, Yu JZ, Jiang Y, Zeller FJ (2003) Genetic polymorphisms at Gli-Dt gliadin loci in Aegilops tauschii as revealed by acid polyacrylamide gel and capillary electrophoresis. Plant Breeding 122, 120–124.
| Genetic polymorphisms at Gli-Dt gliadin loci in Aegilops tauschii as revealed by acid polyacrylamide gel and capillary electrophoresis.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXkvFGktLk%3D&md5=afcbf95fb9db39dd8b72dacaba5cb4a2CAS |
Yu ZT, Han CX, Wang S, Lv DW, Chen GX, Li XH, Jiang GL, Yan YM (2013a) Fast separation and characterization of water-soluble proteins in wheat grains by reversed-phase ultra-performance liquid chromatography (RP-UPLC). Journal of Cereal Science 57, 288–294.
| Fast separation and characterization of water-soluble proteins in wheat grains by reversed-phase ultra-performance liquid chromatography (RP-UPLC).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXhtFKgs78%3D&md5=bc7772dfca57e41568a0353f20710089CAS |
Yu ZT, Han CX, Yan X, Li X, Jiang GL, Yan YM (2013b) Rapid characterization of wheat low molecular weight glutenin subunits by ultra performance liquid chromatography (UPLC). Journal of Agricultural and Food Chemistry 61, 4026–4034.
| Rapid characterization of wheat low molecular weight glutenin subunits by ultra performance liquid chromatography (UPLC).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXlsVertb8%3D&md5=c521ff07a54566792d181f6083d5877bCAS |
Zeller FJ (1973) 1B/1R wheat-rye chromosome substitutions and translocations. In ‘Proceedings of the 4th International Wheat Genetics Symposium’. Record Number: 19751625251. (Eds ER Sears, LMS Sears) pp. 209–221. (Agricultural Experiment Station, College of Agriculture, University of Missouri: Columbia, MO)
Zeller FJ, Hsam SLK (1983) Broadening the genetic variability of cultivated wheat by utilizing rye chromatin. In ‘Proceedings of the 6th International Wheat Genetics Symposium’. (Ed. S Sakamoto) pp. 161–173. (Faculty of Agriculture: Kyoto, Japan)
Zhao CH, Cui F, Wang XQ, Shan SC, Li XF, Bao XG, Wang HG (2012) Effects of 1BL/1RS translocation in wheat on agronomic performance and quality characteristics. Field Crops Research 127, 79–84.
| Effects of 1BL/1RS translocation in wheat on agronomic performance and quality characteristics.Crossref | GoogleScholarGoogle Scholar |
Zhou Y, He ZH, Liu JJ, Liu L (2003) Distribution of 1BL/1RS translocation in Chinese winter wheat and its effect on noodle quality. In ‘Proceedings of 10th International Wheat Genetics Symposium’. (Eds NE Pogna, M Romano, G Galterio). Paestum 3, 1419–1421.
