Identification of QTLs for shoot and root growth under ionic–osmotic stress in Lotus, using a RIL population
Gastón Quero A , Lucía Gutíerrez B , Ramiro Lascano C D , Jorge Monza A , Niels Sandal E and Omar Borsani A F
+ Author Affiliations
- Author Affiliations
A Laboratorio de Bioquímica, Facultad de Agronomía, Universidad de la República, Av. Garzón 780, 12900. Montevideo, Uruguay.
B Departamento de Biometría, Estadística y Computación, Facultad de Agronomía, Universidad de la República, Av. Garzón 780, 12900. Montevideo, Uruguay.
C Instituto de Fisiología y Recursos Genéticos Vegetales CIAP-INTA, Camino 60 Cuadras km 5 (X5020ICA), Córdoba, Argentina.
D Cátedra de Fisiología Vegetal. Universidad Nacional de Córdoba, Av. Vélez Sarsfield 290, 5000. Córdoba, Argentina.
E Centre for Carbohydrate Recognition and Signalling, Department of Molecular Biology and Genetics, Aarhus University, Gustav Wieds Vej 10, DK-8000 Aarhus C, Denmark.
F Corresponding author. Email: oborsani@fagro.edu.uy
Crop and Pasture Science 65(2) 139-149 https://doi.org/10.1071/CP13222
Submitted: 24 June 2013 Accepted: 19 December 2013 Published: 20 February 2014
Abstract
The genus Lotus includes a group of forage legume species including genotypes of agronomic interest and model species. In this work, an experimental hydroponic growth system allowed the discrimination of growth responses to ionic–osmotic stress in a population of recombinant inbred lines (RILs) developed from L. japonicus × L. burttii and the identification of the associated quantitative trait loci (QTLs). The analyses led to the identification of eight QTLs: three for shoot growth localised on chromosome 3, 5 and 6; one for root growth on chromosome 1; three for total growth on chromosome 1, 4 and 5; and one associated with shoot/root ratio on chromosome 3. An interaction of QTL × stress condition was established and the effect of the environment quantified. In summary, it was established that the allele from L. burttii explained most responses to osmotic stress, while the alleles of L. japonicus explained the responses related to ionic stress conditions. Of 49 markers linked to all QTLs identified, 41 expressed superiority of the L. burttii parental allele in the osmotic stress condition, but when an iso-osmotic concentration of NaCl was applied, L. burttii lost superiority in 21 of these markers. This shows the superiority of the L. japonicus parental allele in ionic stress conditions. This study is the first report in which a RIL population of lotus is analysed with the aim of providing molecular markers associated with plant responses to ionic or osmotic stress.
Additional keywords: hydroponic growth, L. japonicus, L. burttii, PEG stress, salt stress.
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