Variability of in vitro ruminal fermentation and methanogenic potential in the pasture legume biserrula (Biserrula pelecinus L.)
Bidhyut Kumar Banik A B C , Zoey Durmic A , William Erskine B F , Phillip Nichols C E , Kioumars Ghamkhar B D and Philip Vercoe A
+ Author Affiliations
- Author Affiliations
A School of Animal Biology, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.
B Centre for Legumes in Mediterranean Agriculture, The University of Western Australia, 35, Stirling Highway, Crawley, WA 6009, Australia.
C School of Plant Biology, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.
D Current Address: Department of Environment and Primary Industries, AgriBio, 5 Ring Road, La Trobe University, Bundoora, Vic. 3083, Australia.
E Department of Agriculture and Food Western Australia, 3 Baron-Hay Court, South Perth, WA 6151, Australia.
F Corresponding author. Email: william.erskine@uwa.edu.au
Crop and Pasture Science 64(4) 409-416 https://doi.org/10.1071/CP13073
Submitted: 22 February 2013 Accepted: 23 May 2013 Published: 2 August 2013
Abstract
Biserrula (Biserrula pelecinus L.) is an important annual pasture legume for the wheatbelt of southern Australia and has been found to have lower levels of methane output than other pasture legumes when fermented by rumen microbes. Thirty accessions of the biserrula core germplasm collection were grown in the glasshouse to examine intra-specific variability in in vitro rumen fermentation, including methane output. One biserrula cultivar (Casbah) was also grown at two field locations to confirm that low methanogenic potential was present in field-grown samples. All of the biserrula accessions had significantly reduced methane [range 0.5–8.4 mL/g dry matter (DM)] output compared with subterranean clover (28.4 mL/g DM) and red clover (36.1 mL/g DM). There was also significant variation in fermentability profiles (except for volatile fatty acids) among accessions of the core collection. Methanogenic potential exhibited 86% broad-sense heritability within the biserrula core collection. The anti-methanogenic and gas-suppressing effect of biserrula was also confirmed in samples grown in the field. In conclusion, biserrula showed variability in in vitro fermentation traits including reduced methane production compared with controls. This bioactivity of biserrula also persists in the field, indicating scope for further selection of biserrula as an elite methane-mitigating pasture.
Additional keywords: Biserrula pelecinus L., fermentability, heritability, methanogenic potential, pasture legume, VFA.
References
Allen D, Dalal R, Rennenberg H, Schmidt S (2011) Seasonal variation in nitrous oxide and methane emissions from subtropical estuary and coastal mangrove sediments, Australia. Plant Biology 13, 126–133.| Seasonal variation in nitrous oxide and methane emissions from subtropical estuary and coastal mangrove sediments, Australia.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXhsFOjsro%3D&md5=f4e55a61cdf59b1af61c6fbb8db3e0e4CAS | 21143733PubMed |
Banik BK (2010) In vitro ruminal methane production varies in selected key pasture species in Australia. Graduate Diploma, School of Plant Biology, University of Western Australia, Perth, Australia.
Beauchemin KA, Kreuzer M, O’Mara F, McAllister TA (2008) Nutritional management for enteric methane abatement: a review. Australian Journal of Experimental Agriculture 48, 21–27.
| Nutritional management for enteric methane abatement: a review.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXovVGn&md5=f03d16f268d1ba923c5d5594962ed453CAS |
Black JN, Wilkinson GN (1963) The role of time of emergence in determining the growth of individual plants in swards of subterranean clover (Trifolium subterraneum L.). Australian Journal of Agricultural Research 14, 628–638.
| The role of time of emergence in determining the growth of individual plants in swards of subterranean clover (Trifolium subterraneum L.).Crossref | GoogleScholarGoogle Scholar |
Bodas R, Lopez S, Fernandez M, García-González R, Rodríguez AB, Wallace RJ, González JS (2008) In vitro screening of the potential of numerous plant species as antimethanogenic feed additives for ruminants. Animal Feed Science and Technology 145, 245–258.
| In vitro screening of the potential of numerous plant species as antimethanogenic feed additives for ruminants.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXpsVKrsb0%3D&md5=4bfc85971dc368b6cfd7e348213819b1CAS |
Broudiscou LP, Papon Y, Broudiscou AF (2000) Effects of dry plant extracts on fermentation and methanogenesis in continuous culture of rumen microbes. Animal Feed Science and Technology 87, 263–277.
| Effects of dry plant extracts on fermentation and methanogenesis in continuous culture of rumen microbes.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXovFSkt7s%3D&md5=bc3aa940aceac53ca64b282e0fc9fa18CAS |
Brown AHD, Hodgkin T, Brown AHD, Hintum TJL, Morales EAV (Eds) (1995) ‘The core collection at the crossroads.’ (John Wiley and Sons: London)
Busquet M, Calsamiglia S, Ferret A, Kamel C (2006) Plant extracts affect in vitro rumen microbial fermentation. Journal of Dairy Science 89, 761–771.
| Plant extracts affect in vitro rumen microbial fermentation.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XhtlWrtLk%3D&md5=939da7f2227e9c694dc86ff4dd5748bcCAS | 16428643PubMed |
Cardozo PW, Calsamiglia S, Ferret A, Kamel C (2004) Effects of natural plant extracts on ruminal protein degradation and fermentation profiles in continuous culture. Journal of Animal Science 82, 3230–3236.
Dear BS, Cregan PD, Murray GM (1993) Comparison of the performance of subterranean clover cultivars in southern New South Wales 1. Persistence, productivity, and seed yields. Australian Journal of Experimental Agriculture 33, 581–590.
| Comparison of the performance of subterranean clover cultivars in southern New South Wales 1. Persistence, productivity, and seed yields.Crossref | GoogleScholarGoogle Scholar |
Demissie A, Bjørnstad Å, Kleinhofs A (1998) Restriction fragment length polymorphisms in landrace barleys from Ethiopia in relation to geographic, altitude, and agro-ecological factors. Crop Science 38, 237–243.
| Restriction fragment length polymorphisms in landrace barleys from Ethiopia in relation to geographic, altitude, and agro-ecological factors.Crossref | GoogleScholarGoogle Scholar |
Donald EJ, Gerald MW, Jon JR, Kornegay ET (1996) ‘Livestock methane: Current emission and mitigation potential.’ (Lewis Publishers: New York)
Durmic Z, Hutton P, Revell D, Emms J, Hughes S, Vercoe P (2010) In vitro fermentative traits of Australian woody perennial plant species that may be considered as potential sources of feed for grazing ruminants. Animal Feed Science and Technology 160, 98–109.
| In vitro fermentative traits of Australian woody perennial plant species that may be considered as potential sources of feed for grazing ruminants.Crossref | GoogleScholarGoogle Scholar |
Flachowsky G (2011) Carbon-footprints for food of animal origin, reduction potentials and research need. Journal of Applied Animal Research 39, 2–14.
| Carbon-footprints for food of animal origin, reduction potentials and research need.Crossref | GoogleScholarGoogle Scholar |
Flachowsky G, Lebzien P (2012) Effects of phytogenic substances on rumen fermentation and methane emissions: A proposal for a research process. Animal Feed Science and Technology 176, 70–77.
| Effects of phytogenic substances on rumen fermentation and methane emissions: A proposal for a research process.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XhtVyksbzK&md5=3e0018a8eecb537861e471d114950fb6CAS |
Garnaut R (2011) Climate Change: Land use—Agriculture and Forestry. Issues Paper 1 for Garnaut Climate Change Review. Garnaut Review Secretariat, East Melbourne.
Ghamkhar K, Revell C, Erskine W (2012) Biserrula pelecinus L.—genetic diversity in a promising pasture legume for the future. Crop & Pasture Science 63, 833–839.
| Biserrula pelecinus L.—genetic diversity in a promising pasture legume for the future.Crossref | GoogleScholarGoogle Scholar |
IPCC (2007) Intergovernmental Panel on Climate Change. In ‘Climate Change 2007: The Physical Science Basis’. (IPCC: Geneva)
Johnson RR (1966) Techniques and procedures for in vitro and in vivo rumen studies. Journal of Animal Science 25, 855–875.
Kumar S, Dubey D (2001) Variability, heritability and correlation studies in grasspea (Lathyrus sativus L.). Lathyrus Lathyrism Newsletter 2, 79–81.
Li C, Berninger F, Koskela J, Sonninen E (2000) Drought responses of Eucalyptus microtheca provenances depend on seasonality of rainfall in their place of origin. Australian Journal of Plant Physiology 27, 231–238.
Loi A, Howieson J, Carr S, Kelman W (2001) Register of Australian Herbage Plant Cultivars: B. Legumes, 25. Biserrula, Biserrula pelecinus L.(biserrula) cv. Casbah. Australian Journal of Experimental Agriculture 41, 841–842.
| Register of Australian Herbage Plant Cultivars: B. Legumes, 25. Biserrula, Biserrula pelecinus L.(biserrula) cv. Casbah.Crossref | GoogleScholarGoogle Scholar |
McDonald P, Edwards RA, Greenhalgh JFD (1988) ‘Animal nutrition.’ (Longman Scientific & Technical: Harlow, UK)
McDougall EI (1948) Studies on ruminant saliva. 1. The composition and output of sheep’s saliva. Biochemical Journal 43, 99–109.
Nichols P, Loi A, Nutt B, Evans P, Craig A, Pengelly B, Dear B, Lloyd D, Revell C, Nair R, Ewing M, Howieson J, Auricht G, Howie J, Sandral G, Carr S, de Koning C, Hackeny B, Crocher G, Snowbell R, Hughes S, Hall E, Foster K, Skinner P, Barbetti M, You M (2007) New annual and short-lived perennial pasture legumes for Australian agriculture–15 years of revolution. Field Crops Research 104, 10–23.
| New annual and short-lived perennial pasture legumes for Australian agriculture–15 years of revolution.Crossref | GoogleScholarGoogle Scholar |
Nichols P, Cocks P, Francis C (2009) Evolution over 16 years in a bulk-hybrid population of subterranean clover (Trifolium subterraneum L.) at two contrasting sites in south-western Australia. Euphytica 169, 31–48.
| Evolution over 16 years in a bulk-hybrid population of subterranean clover (Trifolium subterraneum L.) at two contrasting sites in south-western Australia.Crossref | GoogleScholarGoogle Scholar |
Shibata M, Minami K, Mosier A, Sass R (Eds) (1994) ‘Methane production in ruminants.’ (Yokendo Publishers: Tokyo)
Smith A, Lim P, Cullis B (2006) The design and analysis of multi-phase plant breeding experiments. The Journal of Agricultural Science 144, 393–409.
| The design and analysis of multi-phase plant breeding experiments.Crossref | GoogleScholarGoogle Scholar |
Soliva CR, Zeleke AB, Clément C, Hess HD, Fievez V, Kreuzer M (2008) In vitro screening of various tropical foliages, seeds, fruits and medicinal plants for low methane and high ammonia generating potentials in the rumen. Animal Feed Science and Technology 147, 53–71.
| In vitro screening of various tropical foliages, seeds, fruits and medicinal plants for low methane and high ammonia generating potentials in the rumen.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXht1ejtLzI&md5=75ba61a978c34b3c207febf549eb94c1CAS |
Sun XZ, Waghorn GC, Hoskin SO, Harrison SJ, Muetzel S, Pacheco D (2012) Methane emissions from sheep fed fresh brassicas (Brassica spp.) compared to perennial ryegrass (Lolium perenne). Animal Feed Science and Technology 176, 107–116.
| Methane emissions from sheep fed fresh brassicas (Brassica spp.) compared to perennial ryegrass (Lolium perenne).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XhtVykt7zE&md5=e8e9bc090bd0d4538e602734011d4eb4CAS |
Tavendale MH, Meagher LP, Pacheco D, Walker N, Attwood GT, Sivakumaran S (2005) Methane production from in vitro rumen incubations with Lotus pedunculatus and Medicago sativa, and effects of extractable condensed tannin fractions on methanogenesis. Animal Feed Science and Technology 123–124, 403–419.
| Methane production from in vitro rumen incubations with Lotus pedunculatus and Medicago sativa, and effects of extractable condensed tannin fractions on methanogenesis.Crossref | GoogleScholarGoogle Scholar |
