Breeding lucerne for persistence
J. H. BoutonForage Improvement Division, The Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK 73401, USA. Email: jhbouton@noble.org
Crop and Pasture Science 63(2) 95-106 https://doi.org/10.1071/CP12009
Submitted: 9 January 2012 Accepted: 8 March 2012 Published: 17 April 2012
Journal Compilation © CSIRO Publishing 2012 Open Access CC BY-NC-ND
Abstract
Cultivated lucerne is the most widely grown forage legume in pastoral agriculture. Persistence is critical for most pastoral production systems and its definition includes concepts of productivity, but maintenance of adequate plant numbers is essential. There were three important eras in lucerne persistence breeding: species introduction leading to local varieties and land races (adaptation), development of multiple pest-resistant, autumn dormancy-specific cultivars, and introducing complex traits and the use of biotechnologies. Today’s persistent cultivar needs, at a minimum, adaptation, proper autumn dormancy, and targeted pest resistances. Adding complex, ‘persistence-limiting’ traits to these minimum base traits, such as tolerance to grazing, acid, aluminum-toxic soils, and drought, is successfully being achieved via traditional selection, but biotechnologies and inter-specific hybridisations are also being employed in some cases. The main issues around biotechnologies are public perception and regulatory issues which continue to hamper transgene deployment while genetic marker programs need to lower costs and concentrate on successful application. There is not one persistent ‘ideotype’ that will fill all situations, but specific ones need to be developed and targeted for geographies such as the subtropics. Finally, breeders need to understand what persistence traits lucerne producers are willing to pay a premium to obtain.
Additional keywords: acid soils, alfalfa, drought, grazing, Medicago, pest resistance.
References
Anonymous (2011) Roundup ready alfalfa. Available at: www.monsanto.com/newsviews/Pages/roundup-ready-alfalfa-supreme-court.aspx (accessed 3 January 2012).Barnes DK, Goplen BP, Baylor JE (1988) Highlights in the USA and Canada. In ‘Alfalfa and alfalfa improvement’. (Eds AA Hanson, DK Barnes, RR Hill, Jr.) pp. 1–24. (American Society of Agronomy: Madison, WI)
Barone P, Rosellini D, LaFayette P, Bouton J, Veronesi F, Parrot W (2008) Bacterial citrate synthase expression and soil aluminum tolerance in transgenic alfalfa. Plant Cell Reports 27, 893–901.
| Bacterial citrate synthase expression and soil aluminum tolerance in transgenic alfalfa.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXksFygsrw%3D&md5=73dd83ca8655d65ec4baee903e53182eCAS |
Berdahl JD, Wilton AC, Frank AB (1989) Survival and agronomic performance of 25 alfalfa cultivars and strains interseeded into rangelands. Journal of Range Management 42, 312–316.
| Survival and agronomic performance of 25 alfalfa cultivars and strains interseeded into rangelands.Crossref | GoogleScholarGoogle Scholar |
Beuselinck PR, Bouton JH, Lamp WO, Matches AG, McCaslin MH, Nelson CJ, Rhodes LH, Sheaffer CC, Volenec JJ (1994) Improving legume persistence in forage crop systems. Journal of Production Agriculture 7, 311–322.
Bingham ET (1990) Backcrossing tetraploidy into diploid Medicago falcata L. using 2n eggs. Crop Science 30, 1353–1354.
| Backcrossing tetraploidy into diploid Medicago falcata L. using 2n eggs.Crossref | GoogleScholarGoogle Scholar |
Bingham ET, Groose RW, Woodfield DR, Kidwell KK (1994) Complementary gene interactions in alfalfa are greater in autotetraploids than diploids. Crop Science 34, 823–828.
| Complementary gene interactions in alfalfa are greater in autotetraploids than diploids.Crossref | GoogleScholarGoogle Scholar |
Boschma SP, Williams RW (2008) Using morphological traits to identify persistent lucernes for dryland agriculture in NSW, Australia. Australian Journal of Agricultural Research 59, 69–79.
| Using morphological traits to identify persistent lucernes for dryland agriculture in NSW, Australia.Crossref | GoogleScholarGoogle Scholar |
Bouton JH (1996a) New uses for alfalfa and other ‘old’ forage legumes. In ‘Progress in new crops’. (Ed. J Janick) pp. 251–259. (American Society of Horticultural Science Press: Alexandria, VA)
Bouton JH (1996b) Screening the alfalfa core collection for acid toil tolerance. Crop Science 36, 198–200.
| Screening the alfalfa core collection for acid toil tolerance.Crossref | GoogleScholarGoogle Scholar |
Bouton JH (2001) Alfalfa. In ‘Proceedings XIX International Grassland Congress’. (Eds JA Gomide, WRS Mattos, SC d’Silva) pp. 545–547. (FEALQ, Piracicaba, SP, Brazil: Sao Pedro, Sao Paulo, Brazil)
Bouton J (2007) The economic benefits of forage improvement in the United States. Euphytica 154, 263–270.
| The economic benefits of forage improvement in the United States.Crossref | GoogleScholarGoogle Scholar |
Bouton JH (2010) Future developments and uses. In ‘Handbook of plant breeding: Volume 5. Fodder crops and amenity grasses’. (Eds B Boller, UK Posselt, F Veronesi) pp. 201–209. (Springer: New York)
Bouton JH, Gates RN (2003) Grazing-tolerant alfalfa cultivars perform well under rotational stocking and hay management. Agronomy Journal 95, 1461–1464.
| Grazing-tolerant alfalfa cultivars perform well under rotational stocking and hay management.Crossref | GoogleScholarGoogle Scholar |
Bouton JH, Parrott WA (1997) ‘Salinity and aluminium stress.’. In ‘Biotechnology and the improvement of forage legumes’. (Eds BD McKersie, DCW Brown) pp. 203–226. (CAB International: Wallingford, UK)
Bouton JH, Radcliffe DE (1989) ‘Effects of acid soil selection on agronomically important traits in alfalfa.’ In ‘Proceedings XVI International Grassland Congress’. Nice, France, 4–11 October 1989. pp. 377–378.
Bouton JH, Smith SRJ (1998) Standard test to characterize alfalfa cultivar tolerance to intensive grazing with continuous stocking. Available at: www.naaic.org/stdtests/Grazing.html (accessed 29 December 2011).
Bouton JH, Sumner ME (1983) Alfalfa, Medicago sativa L., in highly weathered, acid soils. V. Field performance of alfalfa selected for acid tolerance. Plant and Soil 74, 431–436.
| Alfalfa, Medicago sativa L., in highly weathered, acid soils. V. Field performance of alfalfa selected for acid tolerance.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL2cXhtVahsrg%3D&md5=2a4879cd7652295e5dc5a4f341f26636CAS |
Bouton JH, Sumner ME, Hammel JE, Shahandeh H (1986) Yield of an alfalfa germplasm selected for acid soil tolerance when grown in soil with modified subsoils. Crop Science 26, 334–336.
| Yield of an alfalfa germplasm selected for acid soil tolerance when grown in soil with modified subsoils.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL28Xhs1Omtrw%3D&md5=0f85749a7d97c66aa47f94ad294d9acbCAS |
Bouton JH, Smith SR, Wood DT, Hoveland CS, Brummer EC (1991) Registration of ‘Alfagraze’ alfalfa. Crop Science 31, 479
| Registration of ‘Alfagraze’ alfalfa.Crossref | GoogleScholarGoogle Scholar |
Bouton JH, Gates RN, Utley PR (1998) Persistence and yield among nondormant alfalfas selected for grazing tolerance. Journal of Production Agriculture 11, 314–318.
Bouton J, McCaslin M, Fitzpatrick S, Temple S (2006) Roundup Ready Alfagraze. Available at: www.naaic.org/Meetings/National/2006meeting/procedings/Bouton(oral).PDF (accessed 29 December 2011).
Brady NC (1974) ‘The nature and property of soils.’ (Macmillan Publishing Co. Inc.: New York)
Brummer EC, Bouton JH (1991) Plant traits associated with grazing-tolerant alfalfa. Agronomy Journal 83, 996–1000.
| Plant traits associated with grazing-tolerant alfalfa.Crossref | GoogleScholarGoogle Scholar |
Brummer EC, Bouton JH (1992) Physiological traits associated with grazing-tolerant alfalfa. Agronomy Journal 84, 138–143.
| Physiological traits associated with grazing-tolerant alfalfa.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK38XktVCnsLs%3D&md5=913f97cfc7486c54908d7ce09f6c8151CAS |
Brummer EC, Bouton JH, Sledge M (2007) Biotechnology and molecular approaches to forage improvement. In ‘Forages. Volume II – the science of grassland agriculture’. 6th edn. (Eds RF Barnes, CJ Nelson, KJ Moore, M Collins) pp. 439–451. (Blackwell Publishing: Ames, IA)
Busbice TH, Hanson AA (1969) Selection for improved creeping-rooted characteristics in alfalfa. Crop Science 9, 244–246.
| Selection for improved creeping-rooted characteristics in alfalfa.Crossref | GoogleScholarGoogle Scholar |
Busbice TH, Wilsie CP (1966) Inbreeding depression and heterosis in autotetraploids with application to Medicago sativa L. Euphytica 15, 52–67.
Caradus JR, Williams WM (1989) Breeding for legume persistence in New Zealand. In ‘Persistence of forage legumes. Proceedings of a Trilateral Workshop’. 18–22 July 1988, Honolulu, Hawaii. (Eds GC Marten, AG Matches, RF Barnes, RW Brougham, RJ Clements, GW Sheath) pp. 523–537. (ASA, CSSA, SSSA: Madison, WI)
Clements RJ (1989) Developing persistent pasture legume cultivars for Australia. In ‘Persistence of forage legumes. Proceedings of a Trilateral Workshop’. 18–22 July 1988, Honolulu, Hawaii. (Eds GC Marten, AG Matches, RF Barnes, RW Brougham, RJ Clements, GW Sheath) pp. 505–521. (ASA, CSSA, SSSA: Madison, WI)
Clements RJ, Turner JW, Irwin JAG, Langdon PW, Bray RA (1984) Breeding disease resistant aphid resistant lucerne Medicago-sativa for subtropical Queensland Australia. Australian Journal of Experimental Agriculture and Animal Husbandry 24, 178–188.
| Breeding disease resistant aphid resistant lucerne Medicago-sativa for subtropical Queensland Australia.Crossref | GoogleScholarGoogle Scholar |
Dall’agnol M, Bouton JH, Parrott WA (1996) Screening methods to develop alfalfa germplasms tolerant of acid, aluminum toxic soils. Crop Science 36, 64–70.
| Screening methods to develop alfalfa germplasms tolerant of acid, aluminum toxic soils.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK28XotVyjsA%3D%3D&md5=0c6999a8c8c8f784210ffa98043dc5e7CAS |
de la Fuente JM, Ramirez-Rodriguez V, Cabrera-Ponce JL, Herrera-Estrella L (1997) Aluminum tolerance in transgenic plants by alteration of citrate synthesis. Science 276, 1566–1568.
| Aluminum tolerance in transgenic plants by alteration of citrate synthesis.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2sXjslGku7Y%3D&md5=ab5ab7b9c0c9a1789a36cef93a3b829bCAS |
Devine TE, Bouton JH, Mabrahtu T (1990) Legume genetics and breeding for stress tolerance and nutrient efficiency. In ‘Crops as enhancers of nutrient use’. (Eds VC Baligar, RR Duncan) pp. 211–252. (Academic Press: San Diego, CA)
Elden TC, McCaslin M (1997) Potato leafhopper (Homoptera: Cicadellidae) resistance in perennial glandular-haired alfalfa clones. Journal of Economic Entomology 90, 842–847.
Gramshaw D, Bishop HG, Ludke DH (1982) Performance of grazed creeping-rooted lucernes on two soils in central Queensland. Australian Journal of Experimental Agriculture and Animal Husbandry 22, 177–181.
| Performance of grazed creeping-rooted lucernes on two soils in central Queensland.Crossref | GoogleScholarGoogle Scholar |
Gramshaw D, Read JW, Collins WJ, Carter ED (1989) Sown pastures and legume persistence: an Australian overview. In ‘Persistence of forage legumes. Proceedings of a Trilateral Workshop’. Honolulu, Hawaii, 18–22 July 1988. (Eds GC Marten, AG Matches, RF Barnes, RW Brougham, RJ Clements, GW Sheath) pp. 1–22. (ASA, CSSA, SSSA: Madison, WI)
Han Y, Ray IM, Sledge MK, Bouton JH, Monteros MJ, Han YH (2008) Drought tolerance in tetraploid alfalfa. In ‘Multifunctional grasslands in a changing world, Volume II: XXI International Grassland Congress and VIII International Rangeland Congress’. Hohhot, China, 29 June–5 July 2008. p.419. (Guangdong People’s Publishing House: Guangzhou, China)
Hanson AA, Barnes DK, Hill RR (1988) ‘Alfalfa and alfalfa improvement.’ (American Society of Agronomy: Madison, WI)
Hartel PG, Bouton JH (1989) Rhizobium meliloti inoculation of alfalfa selected for tolerance to acid aluminum-rich soils. Plant and Soil 116, 283–285.
| Rhizobium meliloti inoculation of alfalfa selected for tolerance to acid aluminum-rich soils.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL1MXlsFaluro%3D&md5=fbb850757e8bfd9507a2b9c9582e48f5CAS |
Hartel PG, Bouton JH (1991) Rhizobium meliloti inoculation of alfalfa selected for tolerance to acid, aluminum-rich soils. In ‘Plant–soil interactions at low pH’. (Eds RJ Wright, VC Baligar, RP Murrmann) pp. 597–601. (Kluwer Academic Publishers: Dordrecht, The Netherlands)
Heinrichs DH (1963) Creeping alfalfas. Advances in Agronomy 15, 317–337.
| Creeping alfalfas.Crossref | GoogleScholarGoogle Scholar |
Henning JC (2000) On-farm use of alfalfa for grazing. In ‘Proceedings/Reports of the American Forage and Grassland Council, 37th North American Alfalfa Improvement Conference’. Madison, Wisconsin, 16–19 July 2000. pp. 359–363.
Hill RR, Jr. (1987) Alfalfa. In ‘Principles of cultivar development, Vol. 2’. (Ed. WR Fehr) pp. 11–39. (Macmillan: New York)
Hoveland CS (1992) Grazing systems for humid regions. Journal of Production Agriculture 5, 23–27.
Humphries AW, Auricht GC, Kobelt ET (2001) Developing grazing tolerant lucerne. Available at: www.regional.org.au/au/asa/2001/6/a/humphries2.htm (accessed 2 January 2012).
Irwin JAG, Lloyd DL, Lowe KF (2001) Lucerne biology and genetic improvement: an analysis of past activities and future goals in Australia. Australian Journal of Agricultural Research 52, 699–712.
| Lucerne biology and genetic improvement: an analysis of past activities and future goals in Australia.Crossref | GoogleScholarGoogle Scholar |
Irwin JAG, Armour DJ, Pepper PM, Lowe KF (2010) Heterosis in lucerne testcrosses with Medicago arborea introgressions and Omani landraces and their performance in synthetics. Crop & Pasture Science 61, 450–463.
| Heterosis in lucerne testcrosses with Medicago arborea introgressions and Omani landraces and their performance in synthetics.Crossref | GoogleScholarGoogle Scholar |
Lawton K (2002) Traffic tolerance. Available at: http://farmindustrynews.com/traffic-tolerance (accessed 29 December 2011).
Leach GJ (1978) The ecology of lucerne pastures. In ‘Plant relations in pastures’. (Ed. JR Wilson) pp. 290–308. (CSIRO Publishing: Melbourne)
Leach GJ, Clements RJ (1984) Ecology and grazing management of alfalfa pastures in the subtropics. Advances in Agronomy 37, 127–154.
| Ecology and grazing management of alfalfa pastures in the subtropics.Crossref | GoogleScholarGoogle Scholar |
Li GD, Lodge GM, Moore GA, Craig AD, Dear BS, Boschma SP, Albertsen TO, Miller SM, Harden S, Hayes RC, Hughes SJ, Snowball R, Smith AB, Cullis BC (2008) Evaluation of perennial pasture legumes and herbs to identify species with high herbage production and persistence in mixed farming zones in southern Australia. Australian Journal of Experimental Agriculture 48, 449–466.
| Evaluation of perennial pasture legumes and herbs to identify species with high herbage production and persistence in mixed farming zones in southern Australia.Crossref | GoogleScholarGoogle Scholar |
Lodge GM (1991) Management practices and other factors contributing to the decline in persistence of grazed lucerne in temperate Australia: a review. Australian Journal of Experimental Agriculture 31, 713–724.
| Management practices and other factors contributing to the decline in persistence of grazed lucerne in temperate Australia: a review.Crossref | GoogleScholarGoogle Scholar |
Mackie JM, Musial JM, Armour DJ, Phan HTT, Ellwood SE, Aitken KS, Irwin JAG (2007) Identification of QTL for reaction to three races of Colletotrichum trifolii and further analysis of inheritance of resistance in autotetraploid lucerne. Theoretical and Applied Genetics 114, 1417–1426.
| Identification of QTL for reaction to three races of Colletotrichum trifolii and further analysis of inheritance of resistance in autotetraploid lucerne.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD2s3nsVWisA%3D%3D&md5=d3a0ab9797d487c6d5645c0ff689af3fCAS |
Marshall EM, Humphries AW, Kobelt ET, Rowe TD, Auricht GC (2008) Drought response of lucerne in South East and Mallee environments in SA. Available at: www.sardi.sa.gov.au/__data/assets/pdf_file/0003/116553/Persistence_Drought_response_of_Lucerne.pdf (accessed 30 December 2011).
Marten GC (1989) Summary of the trilateral workshop on persistence of forage legumes. In ‘Persistence of forage legumes. Proceedings of a Trilateral Workshop’. 18–22 July 1988, Honolulu, Hawaii. (Eds GC Marten, AG Matches, RF Barnes, RW Brougham, RJ Clements, GW Sheath) pp. 569–572. (ASA, CSSA, SSSA: Madison, WI)
Marten GC, Matches AG, Barnes RF, Brougham RW, Clements RJ, Sheath GW (Eds) (1989) Persistence of forage legumes. In ‘Proceedings of a Trilateral Workshop’. Honolulu, Hawaii, 18–22 July 1988. (American Society of Agronomy: Madison, WI)
Moutray JB (2000) Future of alfalfa as a grazing crop: grazing tolerance. In ‘Proceedings/Reports of the American Forage and Grassland Council, 37th North American Alfalfa Improvement Conference’. Madison, Wisconsin, 16–19 July 2000. pp. 345–350.
Musial JM, Mackie JM, Armour DJ, Phan HTT, Ellwood SE, Aitken KS, Irwin JAG (2007) Identification of QTL for resistance and susceptibility to Stagonospora meliloti in autotetraploid lucerne. Theoretical and Applied Genetics 114, 1427–1435.
| Identification of QTL for resistance and susceptibility to Stagonospora meliloti in autotetraploid lucerne.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD2s3nsVWisw%3D%3D&md5=9294d7505d7efe8e45ed5f84b2f94173CAS |
NAAIC (2004) ‘Standard tests to characterize alfalfa cultivars.’ 3rd edn. Available at: www.naaic.org/stdtests/index.html (accessed 29 December 2011).
Narasimhamoorthy B, Bouton JH, Olsen KM, Sledge MK (2007) Quantitative trait loci and candidate gene mapping of aluminum tolerance in diploid alfalfa. Theoretical and Applied Genetics 114, 901–913.
| Quantitative trait loci and candidate gene mapping of aluminum tolerance in diploid alfalfa.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXitVOltrs%3D&md5=93f3219a2d7a58d85d764ee15fe15575CAS |
Parrot WA, Bouton JH (1990) Aluminum tolerance in alfalfa as expressed in tissue culture. Crop Science 30, 387–389.
| Aluminum tolerance in alfalfa as expressed in tissue culture.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3cXksFGqtLs%3D&md5=e46e85926f683046800b2fea090bd848CAS |
Pecetti L, Romani M, De Rosa L, Piano E (2008) Selection of grazing-tolerant lucerne cultivars. Grass and Forage Science 63, 360–368.
| Selection of grazing-tolerant lucerne cultivars.Crossref | GoogleScholarGoogle Scholar |
Piano E, Valentini P, Pecetti L, Romani M (1996) Evaluation of a lucerne germplasm collection in relation to traits conferring grazing tolerance. Euphytica 89, 279–288.
| Evaluation of a lucerne germplasm collection in relation to traits conferring grazing tolerance.Crossref | GoogleScholarGoogle Scholar |
Putnam D, Giannini C, Maciel F, Orloff S, Kirby D, DeBen C, Pattigan D (2010) California alfalfa variety trial yield results including round-up ready varieties. Available at: http://alfalfa.ucdavis.edu/+producing/variety/apr/APR302-2010data.pdf (accessed 30 December 2011).
Quiros CF, Bauchan GR (1988) The genus Medicago and the origin of the Medicago sativa complex. In ‘Alfalfa and alfalfa improvement’. (Eds AA Hanson, DK Barnes, RR Hill, Jr.) pp. 93–124. (American Society of Agronomy: Madison, WI)
Sanchez PA, Salinas JG (1981) Low-input technology for managing oxisols and ultisols in tropical America. Advances in Agronomy 34, 279–406.
| Low-input technology for managing oxisols and ultisols in tropical America.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL38XhtVaqt7s%3D&md5=d06f72108883c0834ecf7e5900eae254CAS |
Sledge MK, Bouton JH, Dall’Agnoll M, Parrott WA, Kochert G (2002) Identification and confirmation of aluminum tolerance QTL in diploid Medicago sativa subsp. coerulea. Crop Science 42, 1121–1128.
| Identification and confirmation of aluminum tolerance QTL in diploid Medicago sativa subsp. coerulea.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XmsVant70%3D&md5=5da7be09e3c6ad854228f66249e7392aCAS |
Smith SR, Bouton JH (1993) Selection within alfalfa cultivars for persistence under continuous stocking. Crop Science 33, 1321–1328.
| Selection within alfalfa cultivars for persistence under continuous stocking.Crossref | GoogleScholarGoogle Scholar |
Smith SR, Bouton JH, Singh A, McCaughey WP (2000) Development and evaluation of grazing-tolerant alfalfa cultivars: a review. Canadian Journal of Plant Science 80, 503–512.
| Development and evaluation of grazing-tolerant alfalfa cultivars: a review.Crossref | GoogleScholarGoogle Scholar |
Sorensen EL, Stuteville DL, Horber EK, Skinner DZ (1994) Registration of KS224 glandular-haired alfalfa germplasm with multiple pest resistance. Crop Science 34, 544
| Registration of KS224 glandular-haired alfalfa germplasm with multiple pest resistance.Crossref | GoogleScholarGoogle Scholar |
Stanford EH (1951) Tetrasomic inheritance in alfalfa. Agronomy Journal 43, 222–225.
| Tetrasomic inheritance in alfalfa.Crossref | GoogleScholarGoogle Scholar |
Stebbins GL (1950) ‘Variation and evolution in plants.’ (Columbia University Press: New York)
Sumner ME, Shahandeh H, Bouton JH, Hammel JE (1986) Amelioration of acid soil profile through deep liming and surface application of gypsum. Soil Science Society of America Journal 50, 1255–1258.
Tesfaye M, Temple SJ, Allan DL, Vance CP, Samac DA (2001) Overexpression of malate dehydrogenase in transgenic alfalfa enhances organic acid synthesis and confers tolerance to aluminum. Plant Physiology 127, 1836–1844.
| Overexpression of malate dehydrogenase in transgenic alfalfa enhances organic acid synthesis and confers tolerance to aluminum.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XjtVWksA%3D%3D&md5=cbab0b4b0f08178000e32d4010732621CAS |
Venkatanagappa S (2008) Breeding lucerne for the Australian environment through a Private Public Partnership. Available at: www.naaic.org/Meetings/National/2008meeting/proceedings/Venkatanagappa.pdf (accessed 29 December 2011).
Zhang J-Y, Broeckling CD, Blancaflor EB, Sledge MK, Sumner LW, Wang Z-Y (2005) Overexpression of WXP1, a putative Medicago truncatula AP2 domain-containing transcription factor gene, increases cuticular wax accumulation and enhances drought tolerance in transgenic alfalfa (Medicago sativa). The Plant Journal 42, 689–707.
| Overexpression of WXP1, a putative Medicago truncatula AP2 domain-containing transcription factor gene, increases cuticular wax accumulation and enhances drought tolerance in transgenic alfalfa (Medicago sativa).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXlt1yns7w%3D&md5=09ebf4f880f4d3ad329173c29160848aCAS |
Zhang X, Humphries A, Auricht G (2007) Genetic variability and inheritance of aluminium tolerance as indicated by long root regrowth in lucerne (Medicago sativa L.). Euphytica 157, 177–184.
| Genetic variability and inheritance of aluminium tolerance as indicated by long root regrowth in lucerne (Medicago sativa L.).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXpslaqu78%3D&md5=39cc446b4bdd26dc237941a8b31f985bCAS |