Molecular genetic marker-based analysis of species-differentiated phenotypic characters in an interspecific ryegrass mapping population
J. Wang A D E , R. C. Baillie B D E , N. O. I. Cogan B D E , N. M. McFarlane A D , M. P. Dupal B D , K. F. Smith A C D and J. W. Forster B C D E F
+ Author Affiliations
- Author Affiliations
A Department of Primary Industries, Biosciences Research Division, Hamilton Centre, Mount Napier Road, Hamilton, Vic. 3300, Australia.
B Department of Primary Industries, Biosciences Research Division, Victorian AgriBiosciences Centre, 1 Park Drive, La Trobe University Research and Development Park, Bundoora, Vic. 3083, Australia.
C La Trobe University, Bundoora, Vic. 3086, Australia.
D Molecular Plant Breeding Cooperative Research Centre, Victorian AgriBiosciences Centre, 1 Park Drive, La Trobe University Research and Development Park, Bundoora, Vic. 3083, Australia.
E Dairy Futures Cooperative Research Centre, Victorian AgriBiosciences Centre, 1 Park Drive, La Trobe University Research and Development Park, Bundoora, Vic. 3083, Australia.
F Corresponding author. Email: john.forster@dpi.vic.gov.au
Crop and Pasture Science 62(10) 892-902 https://doi.org/10.1071/CP11199
Submitted: 26 July 2011 Accepted: 21 October 2011 Published: 6 December 2011
Abstract
The genus Lolium (ryegrasses) exhibits substantial variation between species for annual-perennial growth habit. The genetic bases of this trait, and other characters that are differentiated between taxa, have been investigated through molecular genetic marker-based mapping of an interspecific mapping population derived from pair-wise crossing of single genotypes from Lincoln, a long-lived cultivar of perennial ryegrass, and Andrea, a cultivar of annual-type Italian ryegrass. The Andrea1246 and Lincoln1133 parental maps contained 122 loci on eight linkage groups (LGs), and 169 loci on seven LGs, respectively. A total of 10 phenotypic traits were measured, including annuality-perenniality index, date of head emergence, number of spikes per plant, number of spikelets per spike, number of floret per spike, flag leaf length, flag leaf width, spike length, stem length, and extent of regrowth. A total of 31 putative quantitative trait loci (QTLs) were detected. Regions of significance were identified on Andrea1246 LGs 1, 2, 3 and 6. An annuality-perenniality index QTL on LG2 accounted for ~30% of trait-specific phenotypic variance (Vp). In addition, LG2 contained coincident QTLs for the number of spikes per plant and head emergence date traits. The Lincoln1133 genetic map displayed QTL-containing regions of significance on LGs 1, 4, 5 and 7, accounting individually for 10–22% of Vp. QTLs identified in this study provide potential targets for ryegrass breeding in order to improve vegetative yield, persistence and seed yield.
Additional keywords: annual, Lolium, molecular breeding, perennial, quantitative trait locus, simple sequence repeat.
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