Genetic diversity between ex situ and in situ samples of Agropyron cristatum (L.) Gaertn. based on simple sequence repeat molecular markers
Y. H. Che A B C , Y. P. Yang B , X. M. Yang A , X. Q. Li A and L. H. Li A C
+ Author Affiliations
- Author Affiliations
A Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, China.
B Department of Life Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066600, Hebei, China.
C Corresponding author. Email: 13933502823@163.com; lilihui@caas.net.cn
Crop and Pasture Science 62(8) 639-644 https://doi.org/10.1071/CP11065
Submitted: 14 March 2011 Accepted: 19 August 2011 Published: 13 September 2011
Abstract
Fifty-five populations of Agropyron cristatum (L.) Gaertn., both ex situ conserved and in situ collected and originating from northern China, were analysed using 29 polymorphic simple sequence repeat (SSR) primers mapped on the wheat genome. The study showed ex situ and in situ populations had different numbers of alleles and allelic frequencies in 17 of the 29 primer pairs. The SSR markers revealed significant genetic differences between gene bank-conserved and in situ recollected populations from similar ecogeographical regions. AMOVA analysis indicated 15% of the total variation resided between groups, and 85% resided within groups. According to a dendrogram generated by unweighted pair group method with arithmetic average cluster analysis based on Nei’s genetic distance matrix and principal coordinate analysis, recollected and gene bank-conserved populations from the same regions were distinguished. The results indicate that both in situ and ex situ conservation play complementary roles in the conservation of A. cristatum.
Additional keywords: Agropyron cristatum, ex situ, genetic variation, recollection in situ, SSR.
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