Development of an allele-specific marker for Glu-B3 alleles in common wheat and establishment of a multiplex PCR assay
Xinxia Sui A B F , Linhai Wang A F , Xianchun Xia A , Zhenlin Wang C and Zhonghu He A D E
+ Author Affiliations
- Author Affiliations
A Institute of Crop Science, National Wheat Improvement Centre/The National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing 100081, China.
B Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
C College of Life Sciences, Shandong Agricultural University, Taian 271018, China.
D International Maize and Wheat Improvement Centre (CIMMYT) China Office, c/o CAAS, 12 Zhongguancun South Street, Beijing 100081, China.
E Corresponding author. Email: zhhecaas@163.com or zhhecaas@gmail.com
F Xinxia Sui and Linhai Wang contributed equally to this work.
Crop and Pasture Science 61(12) 978-987 https://doi.org/10.1071/CP10241
Submitted: 16 July 2010 Accepted: 29 October 2010 Published: 8 December 2010
Abstract
Low-molecular-weight glutenin subunits (LMW-GS) have significant effects on the processing quality of end-use products of common wheat. It is more efficient to discriminate LMW-GS alleles with PCR-based molecular markers than with SDS-PAGE. In the present study, we developed an allele-specific PCR marker, designated Glu-B3abefg, which can be used to discriminate protein alleles Glu-B3a, b, e, f, and g simultaneously. Based on ten previously developed allele-specific STS markers, three multiplex PCRs, viz. Glu-B3c + Glu-B3d, Glu-B3b + Glu-B3g, and Glu-B3h + Glu-B3i, were established. Six Glu-B3 alleles (b, c, d, g, h, and i) could be discriminated using the three multiplex PCRs. Results of tests on 158 wheat varieties and lines using Glu-B3abefg and the three multiplex PCRs were consistent with those using the ten STS markers separately. The new allele-specific marker and three multiplex PCRs represent an efficient way to undertake marker-assisted selection of Glu-B3 alleles.
Additional keywords: Functional marker, LMW-GS, multiplex PCR, Triticum aestivum L.
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