Chromosome composition of an F2 Triticum aestivum × T. turgidum spp. durum cross analysed by DArT markers and MCFISH
Friederike S. Eberhard A , Peng Zhang B , Anke Lehmensiek A , Ray A. Hare C , Steven Simpfendorfer C and Mark W. Sutherland A DA Centre for Systems Biology, University of Southern Queensland, Toowoomba, Qld 4350, Australia.
B University of Sydney, Plant Breeding Institute, Cobbitty, NSW 2570, Australia.
C New South Wales Industry and Investment, Tamworth Agricultural Institute, Tamworth, NSW 2340, Australia.
D Corresponding author. Email: mark.sutherland@usq.edu.au
Crop and Pasture Science 61(8) 619-624 https://doi.org/10.1071/CP10131
Submitted: 16 April 2010 Accepted: 20 June 2010 Published: 13 August 2010
Abstract
This study has employed multicolour fluorescence in situ hybridisation (MCFISH) and Diversity Arrays Technology (DArT) markers to determine the segregation of parental A, B and D genome material into the progeny of a cross between a hexaploid bread wheat (Triticum aestivum L. var. 2-49) and a tetraploid durum wheat [T. turgidum L. spp. durum (Desf.) var. Bellaroi]. In the F2 progeny from a 2-49/Bellaroi cross, 82 out of 83 F2 plants investigated with DArT analysis carried some D genome material, principally as entire chromosomes, while 40 plants included at least one complete copy of all seven D genome chromosomes. Twelve plants containing partial D chromosomes were identified. MCFISH analysis of 26 additional F2 plants of the same cross showed that all 26 plants contained varying amounts of D genome material of which three carried single A-D translocations. In addition two telocentric D genome chromosomes were detected. The D genome content of each line and the breakpoint positions of the three A-D translocations were confirmed with DArT marker analysis. Overall results indicate a random recombination of A and B genome loci from the hexaploid female parent and the tetraploid male parent in this F2 population and a significant retention of the maternal D genome material. This study illustrates that the combined application of the MCFISH and DArT techniques provides a powerful approach for the analysis of crosses between cereal genotypes of different ploidy.
Additional keywords: DArT, durum wheat, hexaploid × durum crosses, MCFISH.
Acknowledgments
The authors would like to thank Professor Grant Daggard (USQ) for his critical reading of the manuscript. This work was funded by the Grains Research and Development Corporation, Australia.
Akbari M,
Wenzl P,
Caig V,
Carling J,
Xia L,
Yang S,
Uszynski G,
Mohler V,
Lehmensiek A,
Kuchel H,
Hayden MJ,
Howes N,
Sharp P,
Vaughan P,
Rathmell B,
Huttner E, Kilian A
(2006) Diversity arrays technology (DArT) for high-throughput profiling of the hexaploid wheat genome. Theoretical and Applied Genetics 113, 1409–1420.
| Crossref | GoogleScholarGoogle Scholar |
CAS |
PubMed |
Aoki T, O’Donnell K
(1999) Morphological and molecular characterization of Fusarium pseudograminearum sp. nov., formerly recognized as the Group 1 population of F. graminearum. Mycologia 91, 597–609.
| Crossref | GoogleScholarGoogle Scholar |
Boeuf C,
Prodanovic G,
Gay G, Bernard M
(2003) Structural organization of the group-1 chromosomes of two bread wheat sister lines. Theoretical and Applied Genetics 106, 938–946.
| Crossref | GoogleScholarGoogle Scholar |
CAS |
PubMed |
Ceoloni C,
Biagetti M,
Ciaffi M,
Forte P, Pasquini M
(1996) Wheat chromosome engineering at the 4× level: the potential of different alien gene transfers into durum wheat. Euphytica 89, 87–97.
| Crossref | GoogleScholarGoogle Scholar |
CAS |
Collard BCY,
Grams RA,
Bovill WD,
Percy CD,
Jolley R,
Lehmensiek A,
Wildermuth GB, Sutherland MW
(2005) Development of molecular markers for crown rot resistance in wheat: mapping of QTLs for seedling resistance in a ‘2-49’ × ‘Janz’ population. Plant Breeding 124, 532–537.
| Crossref | GoogleScholarGoogle Scholar |
CAS |
Gilbert J,
Procunier JD, Aung T
(2000) Influence of the D genome in conferring resistance to fusarium head blight in spring wheat. Euphytica 114, 181–186.
| Crossref | GoogleScholarGoogle Scholar |
CAS |
Kihara H
(1925) Weitere Untersuchungen über die pentaploiden Triticum-Bastarde. I. Japanese Journal of Botany 2, 299–305.
Kumar S,
Gill BS, Faris JD
(2007) Identification and characterization of segregation distortion loci along chromosome 5B in tetraploid wheat. Molecular Genetics and Genomics 278, 187–196.
| Crossref | GoogleScholarGoogle Scholar |
CAS |
PubMed |
Lanning SP,
Blake NK,
Sherman JD, Talbert LE
(2008) Variable production of tetraploid and hexaploid progeny lines from spring wheat by durum wheat crosses. Crop Science 48, 199–202.
| Crossref | GoogleScholarGoogle Scholar |
Manly KF,
Cudmore RHJ, Meer JM
(2001) Map Manager QTX, cross-platform software for genetic mapping. Mammalian Genome 12, 930–932.
| Crossref | GoogleScholarGoogle Scholar |
CAS |
PubMed |
Sax K
(1922) Sterility in wheat hybrids II: Chromosome behaviour in partially sterile hybrids. Genetics 7, 513–585.
|
CAS |
PubMed |
Thompson WP, Hollinghead I
(1927) Preponderance of dicoccum-like characters and chromosome numbers in hybrids between T. dicoccum and T. vulgare. Genetics 17, 283–307.
| Crossref | GoogleScholarGoogle Scholar |
Van Os H,
Stam P,
Visser RGF, Van Eck HJ
(2005) RECORD: a novel method for ordering loci on a genetic linkage map. Theoretical and Applied Genetics 112, 30–40.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Wang HY,
Liu DC,
Yan ZH,
Wei YM, Zheng YL
(2005) Cytological characteristics of F2 hybrids between Triticum aestivum L. and T. durum Desf. with reference to wheat breeding. Journal of Applied Genetics 46, 365–369.
| PubMed |
Zhang P,
Friebe B,
Lukaszewski AJ, Gill BS
(2001) The centromere structure in Robertsonian wheat-rye translocation chromosomes indicates that centric breakage-fusion can occur at different positions within the primary constriction. Chromosoma 110, 335–344.
| Crossref | GoogleScholarGoogle Scholar |
CAS |
PubMed |
Zhang P,
Li W,
Friebe B, Gill BS
(2004) Simultaneous painting of three genomes in hexaploid wheat by BAC-FISH. Genome 47, 979–987.
| Crossref | GoogleScholarGoogle Scholar |
CAS |
PubMed |
