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RESEARCH ARTICLE
Contents Vol 61(9)

Coincident quantitative trait loci effects for dormancy, water sensitivity and malting quality traits in the BCD47 × Baronesse barley mapping population

Ariel Julio Castro A E , Andrea Benitez A , Patrick M. Hayes B , Luis Viega C and Les Wright D
+ Author Affiliations
- Author Affiliations

A Departamento de Producción Vegetal, Est. Exp. ‘Dr Mario A. Cassinoni’, Facultad de Agronomía, Universidad de la República, Ruta 3 Km 363, Paysandú 60000, Uruguay.

B Barley Project, Department of Crop and Soil Sciences, Oregon State University, Corvallis, OR 97331, USA.

C Departamento Biología Vegetal, Facultad de Agronomía, Universidad de la República, Garzón 780, Montevideo 12900, Uruguay.

D Busch Agricultural Resources Inc., 3515 Richards Lake Road, Fort Collins, CO 80524, USA.

E Corresponding author. Email: vontruch@fagro.edu.uy

Crop and Pasture Science 61(9) 691-699 https://doi.org/10.1071/CP10085
Submitted: 9 March 2010  Accepted: 15 July 2010   Published: 9 September 2010

Abstract

A degree of seed dormancy (SD) is required for malting barley varieties in Uruguay, and many other parts of the world, in order to prevent pre-harvest sprouting. Water sensitivity (WS) (a decrease in germination under excess water) is a related trait that can create problems at the malthouse. Both traits are affected by environmental conditions during grain filling. We used a population of 100 doubled haploid lines derived from the cross BCD47 × Baronesse to map quantitative trait loci (QTL) affecting SD, WS, and malting quality traits. Preliminary experiments revealed that BCD47 has low SD and Baronesse has high SD. WS for these accessions was not known before this research. A major SD QTL – detected in four experiments – is on chromosome 5H, with BCD47 contributing the low dormancy allele. Four other regions with QTL effects for SD were mapped, but these QTL were significant in data from only one or two environments. Four regions were detected with QTL effects for WS, but only two – in 5H coincident with the SD QTL, and 3H – were significant in more than one environment. SD and WS were affected by the average temperature at the end of the grain-filling period, with higher temperatures associated with lower values for SD. At the same region on 5H where SD and WS QTL were detected, we found significant QTL for malt extract, α-amylase activity, β-glucans, FAN, Kolbach index, wort turbidity and protein content, with BCD47 contributing favourable alleles for all traits. These results underscore the importance of environmental effects on both SD and WS as well as the difficulties of combining good malting quality with adequate levels of SD and WS.

Additional keywords: Hordeum vulgare, malting quality, QTL analysis, seed dormancy, water sensitivity.


Acknowledgments

This research was funded by a competitive grant from the Fondo Clemente Estable (FCE-9025) (MEC-DICYT, Uruguay) and the Mesa Nacional de Entidades de Cebada, Uruguay. The authors thank Silvana Gonzalez, Luis Galante and Justino Baladao for their help in processing the samples from the different experiments.


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