Register      Login
Crop and Pasture Science Crop and Pasture Science Society
Plant sciences, sustainable farming systems and food quality
Shopping Cart: ( empty )
You are here: Home > Journals > CP > CP08392
RESEARCH ARTICLE
Contents Vol 60(6)

QTL mapping for milling, gluten quality, and flour pasting properties in a recombinant inbred line population derived from a Chinese soft × hard wheat cross

Yelun Zhang A B , Yunpeng Wu A , Yonggui Xiao A , Jun Yan C , Yong Zhang A , Yan Zhang A , Chuanxi Ma B , Xianchun Xia A E and Zhonghu He A D E
+ Author Affiliations
- Author Affiliations

A Institute of Crop Science, National Wheat Improvement Centre/The National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing 100081, China.

B College of Agronomy, Anhui Agricultural University, Hefei, Anhui 230036, China.

C Cotton Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Huanghedadao, Anyang 455000, Henan Province, China.

D International Maize and Wheat Improvement Centre (CIMMYT) China Office, c/o CAAS, 12 Zhongguancun South Street, Beijing 100081, China.

E Corresponding authors. Email: xiaxianchun@caas.net.cn (Xianchun Xia) or zhhe@public3.bta.net.cn (Zhonghu He)

Crop and Pasture Science 60(6) 587-597 https://doi.org/10.1071/CP08392
Submitted: 3 November 2008  Accepted: 11 March 2009   Published: 12 June 2009

Abstract

Improvement of processing quality is important for various wheat-based end products. A recombinant inbred line (RIL) population derived from a cross between the Chinese wheat cvv. PH82-2 (hard) and Neixiang 188 (soft) was sown at 3 locations across two seasons to map quantitative trait loci (QTLs) for milling, gluten quality, flour pasting properties, and Chinese white salted noodle (CWSN) qualities. One hundred and eighty-eight microsatellite loci, one rye secalin marker Sec1, one STS marker YP7A, one CAPs marker for the Pinb-D1b allele, and four glutenin subunit markers were used to genotype the population and construct a linkage map for subsequent QTL analysis. In total, 53 QTLs for 16 quality parameters were mainly mapped to glutenin loci Glu-A3 (Glu-A3a:Glu-A3d), Glu-B1 (Bx7+By9:Bx14+By15), and Glu-D1 (Bx2+By12:Bx5+By10), and the grain hardness (Pinb-D1a:Pinb-D1b) locus. The-high-molecular weight glutenin subunits (HMW-GS) 5+10 at the Glu-D1 locus showed large effects on mixograph peak time (MPT), mixograph 8 min width (MTxW), and weakening slope (WS), accounting for 43.1%, 24.2%, and 39.7% of the phenotypic variance, respectively. In contrast, the 1RS (1B.1R translocation) showed large negative effects on MTxW, explaining 42.2% of the phenotypic variance. Two important QTLs were detected for Rapid Viscosity Analyzer (RVA) parameters: one for RVA final viscosity (RFV) near the 1RS and the other for RVA setback (RSb) associated with the Glu-B1 locus, explaining 21.6% and 12.3% of the phenotypic variance, respectively. Two QTLs for noodle adhesiveness were identified: one occurred on chromosome 1A and the other was associated with Glu-B1. Two QTLs for noodle springiness, one associated with the 1RS showing a negative effect on noodle quality and the other mapped to the Ha locus on chromosome 5DS, accounted for 9.4% and 8.1% of the phenotypic variance, respectively. In addition, the Ha locus also showed large effects on flower protein content (FPC), mixograph peak width (MPW), and RVA parameters, especially RVA pasting temperature (RPT), explaining 71.5% of the phenotypic variance.

Additional keywords: bread wheat, quantitative trait locus (QTL), quality parameters, molecular markers.


Acknowledgments

The authors are very grateful to Prof. R. A. McIntosh, Plant Breeding Institute, University of Sydney, Australia, for kindly reviewing this manuscript. This study was supported by the National Science Foundation of China (30771335 and 30830072), the National Basic Research Program (2009CB118300), and National 863 Programs (2006AA10Z1A7 and 2006AA100102).


References


AACC (1995) ‘Approved methods of the American Association of Cereal Chemists.’ 9th edn (AACC: St Paul, MN)

Arbelbide M, Bernardo R (2006) Mixed-model QTL mapping for kernel hardness and dough strength in bread wheat. Theoretical and Applied Genetics 112, 885–890.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Badu R, Nair SK, Prasanna BM, Gupta HS (2004) Integrating marker-assisted selection in crop breeding–prospects and challenges. Current Science 87, 607–619. open url image1

Baik BK, Czuchajowska Z, Pomeranz Y (1994) Role and contribution of starch and protein contents and quality to texture profile analysis of oriental noodles. Cereal Chemistry 71, 315–320.
|  CAS |
open url image1

Barreto HJ , Edmeades GO , Chapman SC , Crossa J (1997) The alpha lattice design in plant breeding and agronomy: Generation and analysis. In ‘Developing Drought and Low N-Tolerant Maize. Proceedings of Symposium’. CIMMYT, Mexico. (Eds GO Edmeades, M Banziger, HR Mickelson, CB Peña-Valdivia) pp. 25–29. (CIMMYT: Mexico, D.F.)

Campbell KG, Bergman CJ, Gualberto DG, Anderson JA, Giroux MJ, Hareland G, Fulcher RG, Sorrells ME, Finney PL (1999) Quantitative trait loci associated with kernel traits in a soft by hard wheat cross. Crop Science 39, 1184–1195.
|  CAS |
open url image1

Campbell KG, Finney PL, Bergman CJ, Gualberto DG, Anderson JA , et al . (2001) Quantitative trait loci associated with milling and baking quality in a soft × hard wheat cross. Crop Science 41, 1275–1285.
|  CAS |
open url image1

Chen DS, Kiribuchi-Otobe C, Xu ZH, Chen XM, Zhou Y, He ZH, Yoshida H, Zhang Y, Wang DS (2005) Effect of Wx-A1, Wx-B1 and Wx-D1 protein on starch properties and Chinese fresh noodle quality. Science Agriculture Sinica 38, 856–873. open url image1

Chen F, He ZH, Chen DS, Zhang CL, Zhang Y, Xia XC (2007) Influence of puroindoline alleles on milling performance and qualities of Chinese noodles, steamed bread and pan bread in spring wheats. Journal of Cereal Science 45, 59–66.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Chmielewicz KM , Manly KF (2002) ‘Software for genetic mapping of Mendelian markers and quantitative trait loci.’ (Roswell Park Cancer Institute) Available at: http://www.mapmanager.org/

Crosbie GB (1991) The relationship between starch swelling properties, paste viscosity and boiled noodle quality in wheat flours. Journal of Cereal Science 13, 145–150.
|  CAS |
open url image1

Dhaliwal AS, MacRitchie F (1990) Contribution of protein fractions to dough handling properties of wheat-rye translocation cultivars. Journal of Cereal Science 12, 113–122.
|  CAS |
open url image1

Elouafi I, Nachit MM (2004) A genetic linkage map of the durum × Triticum dicoccoides backcross population based on SSRs and AFLP markers, and QTL analysis for milling traits. Theoretical and Applied Genetics 108, 401–413.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Fenn D, Lukow OM, Bushuk W, DePauw RM (1994) Milling and baking quality of 1BL/1RS translocation wheats I. Effects of genotype and environment. Cereal Chemistry 71, 189–195. open url image1

Galande AA, Tiwari R, Ammiraju JSS, Santra DK, Lagu MD, Rao VS, Gupta VS, Misra BK, Nagarajan S, Ranjekar PK (2001) Genetic analysis of kernel hardness in bread wheat using PCR-based markers. Theoretical and Applied Genetics 103, 601–606.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Gilmour AR, Cullis BR, Verbyla AP (1997) Accounting for natural and extraneous variation in the analysis of field experiments. Journal of Agricultural, Biological & Environmental Statistics 2, 269–273.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Graybosch RA, Peterson CJ, Chung OK (1999) Quality effects of rye (Secale cereale L.) chromosome arm 1RL transferred to wheat (Triticum aestivum L.). Journal of Cereal Science 29, 211–216.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Groos C, Bervas E, Charmet G (2004) Genetic analysis of grain protein content, grain hardness and dough rheology in a hard × hard bread wheat progeny. Journal of Cereal Science 40, 93–100.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

He ZH, Liu L, Xia XC, Liu JJ, Peña RJ (2005) Composition of HMW and LMW glutenin subunits and their effects on dough properties, pan bread, and noodle quality of Chinese bread wheats. Cereal Chemistry 82, 345–350.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

He ZH, Yang J, Zhang Y, Quail KJ, Peña RJ (2004) Pan bread and dry white Chinese noodle quality in Chinese winter wheats. Euphytica 139, 257–267.
| Crossref | GoogleScholarGoogle Scholar | open url image1

He XY, Zhang YL, He ZH, Wu YP, Xiao YG, Ma CX, Xia XC (2008) Characterization of phytoene synthase 1 gene (Psy1) located on common wheat chromosome 7A and development of a functional marker. Theoretical and Applied Genetics 116, 213–221.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Hogg AC, Beecher B, Martin JM, Meyer F, Talbert L, Lanning S, Giroux MJ (2005) Hard wheat milling and bread baking traits affected by the seed-specific overexpression of puroindolines. Crop Science 45, 871–878.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Holland JB, Nyquist WE, Cervantes-Martínez CT (2003) Estimating and interpreting heritability for plant breeding: an update. Plant Breeding Reviews 22, 9–112. open url image1

Hook SCW (1984) Specific weight and wheat quality. Journal of the Science of Food and Agriculture 35, 1136–1141.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Hrušková M, Faměra O (2003) Prediction of wheat and flour Zeleny sedimentation value using NIR technique. Czech Journal of Food Sciences 21, 91–96. open url image1

Huang XQ, Cloutier S, Lycar L, Radovanovic N, Humphreys DG, Noll JS, Somers DJ, Brown PD (2006) Molecular detection of QTLs for agronomic and quality traits in a doubled haploid population derived from two Canadian wheats (Triticum aestivum L.). Theoretical and Applied Genetics 113, 753–766.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Khatkar BS, Bell AE, Schofield JD (1996) A comparative study of the inter-relationships between mixograph parameters and bread-making qualities of wheat flours and glutens. Journal of the Science of Food and Agriculture 72, 71–85.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Kinaci E, Kinaci G (2004) Quality and yield losses due to sunn pest (Hemiptera: Scutelleridae) in different wheat types in Turkey. Field Crops Research 89, 187–195.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Konik CM, Miskelly DM, Gras PW (1992) Contribution of starch and non-starch parameters to the eating quality of Japanese white salted noodles. Journal of the Science of Food and Agriculture 58, 403–406.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Kunert A, Naz AA, Dedeck O, Pillen K, Léon J (2007) AB-QTL analysis in winter wheat: I. Synthetic hexaploid wheat (T. turgidum ssp. dicoccoides × T. tauschii) as a source of favourable alleles for milling and baking quality traits. Theoretical and Applied Genetics 115, 683–695.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Lelley T, Eder C, Grausgruber H (2004) Influence of 1BL.1RS wheat–rye chromosome translocation on genotype by environment interaction. Journal of Cereal Science 39, 313–320.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

León AE, Barrera GN, Perez GT, Ribotta PD, Rosell CM (2006) Effect of damaged starch levels on flour-thermal behaviour and bread staling. European Food Research and Technology 224, 187–192.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Li HH, Ye GY, Wang JK (2007) A modified algorithm for the improvement of composite interval mapping. Genetics 175, 361–374.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Liu JJ, He ZH, Yang J, Xu ZH, Liu AF, Zhao ZD (2003a) Variation of starch properties in wheat cultivars and their relationship with dry white Chinese noodle quality. Science Agriculture Sinica 36, 7–12. open url image1

Liu JJ, He ZH, Zhao ZD, Peña RJ, Rajaram S (2003b) Wheat quality traits and quality parameters of cooked dry white Chinese noodles. Euphytica 131, 147–154.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Liu L, He ZH, Yan J, Zhang Y, Xia XC, Peña RJ (2005) Allelic variation at the Glu-1 and Glu-3 loci, presence of the 1B.1R translocation, and their effects on mixographic properties in Chinese bread wheats. Euphytica 142, 197–204.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Lukaszewski AJ (2000) Manipulation of the 1RS.1BL translocation in wheat by induced homoeologous recombination. Crop Science 40, 216–225.
|  CAS |
open url image1

Martin DJ, Stewart BG (1990) Dough stickiness in rye-derived wheat cultivars. Euphytica 51, 77–86.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Martinant JP, Nicolas Y, Bouguennec A, Popineau Y, Saulnier L, Branlard G (1998) Relationships between mixograph parameters and indices of wheat grain quality. Journal of Cereal Science 27, 179–189.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

McCartney CA, Somers DJ, Humphreys DG, Lukow O, Ames N, Noll J, Cloutier S, McCallum BD (2005) Mapping quantitative trait loci controlling agronomic traits in the spring wheat cross RL4452 × ‘AC Domain’. Genome 48, 870–883.
|  CAS | PubMed |
open url image1

McCartney CA, Somers DJ, Lukow O, Ames N, Noll J, Cloutier S, Humphreys DG, McCallum BD (2006) QTL analysis of quality traits in the spring wheat cross RL4452בAC Domain’. Plant Breeding 125, 565–575.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Miura H, Tannii S, Nakamura T, Watanabe N (1994) Genetic control of amylose content in wheat endosperm starch and differential effects of three Wx genes. Australian Journal of Agricultural Research 89, 179–184. open url image1

Nakamura H (2000) The high-molecular-weight glutenin subunit composition of Japanese hexaploid wheat landraces. Australian Journal of Agricultural Research 51, 673–677.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Nakamura H (2002) Quality evaluation of common wheat endosperm protein fingerprints as indices of Japanese white-salted noodle (udon) eating quality. Annual Wheat Newsletter Vol. 48. Available at: http://wheat.pw.usda.gov/ggpages/awn/48/Textfiles/ JAPAN.html

Panozzo JF, McCormick KM (1993) The Rapid Viscosity Analyser as a method of testing for noodle quality in a wheat breeding programme. Journal of Cereal Science 17, 25–32.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Payne PI (1988) Endosperm proteins. In ‘Plant gene research: a genetic approach to plant biochemistry’. (Eds AD Blonstein, PJ King) (Plant Breeding Institute: Cambridge, UK)

Perretant MR, Cadalen T, Charmet G, Sourdille P, Nicolas P, Boeuf C, Tixier MH, Branlard G, Bernard S, Bernard M (2000) QTL analysis of bread-making quality in wheat using a doubled haploid population. Theoretical and Applied Genetics 100, 1167–1175.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Pestsova E, Ganal MW, Röder MS (2000) Isolation and mapping of microsatellite markers specific for the D genome of bread wheat. Genome 43, 689–697.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier MH, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149, 2007–2023.
| PubMed |
open url image1

Rogers DE, Hoseney RC, Lookhart GL, Curran SP, Lin WDA, Sears RG (1993) Milling and cookie baking quality of near-isogenic lines of wheat differing in kernel hardness. Cereal Chemistry 70, 183–187.
|  CAS |
open url image1

Sambrook J , Fritsch EF , Maniatis T (1989) ‘Molecular cloning: a laboratory manual.’ 2nd edn (Cold Spring Habour Laboratory Press: New York)

Shi JR , Song QJ , Singh S , Lewis J , Ward RW , Cregan P , Gill BS (2003) Genetic and physical maps of Xbarc SSR loci in wheat. In ‘Proceedings of National Fusarium Head Blight Forum’. Bloomington, MN, 13–15 Dec. 2003. (Eds SM Canty et al.) pp. 41–45. (Michigan State University: East Lansing, MI)

Singh NK, Shepherd KW, Cornish GB (1991) A simplified SDS-PAGE procedure for separating LMW subunits of glutenin. Journal of Cereal Science 14, 203–208. open url image1

Singh NK, Shepherd KW, McIntosh RA (1990) Linkage mapping of genes for resistance to leaf, stem and stripe rusts and ω-secalins on the short arm of rye chromosome 1R. Theoretical and Applied Genetics 80, 609–616.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Somers DJ, Isaac P, Edwards K (2004) A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theoretical and Applied Genetics 109, 1105–1114.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Song QJ, Fickus EW, Cregan PB (2002) Characterization of trinucleotide SSR motifs in wheat. Theoretical and Applied Genetics 104, 286–293.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Villareal RL, Bañuelos O, Mujeeb-Kazi A, Rajaram S (1998) Agronomic performance of chromosomes 1B and T1BL.1RS near-isolines in the spring bread wheat Seri M82. Euphytica 103, 195–202.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Wang R, Li SB, Wang GR, Zhao CP (1995) Association between wheat quality and quantity of bread, noodle and steamed bread. Agronomy Abroad-Wheat Science 3, 35–37.
|  CAS |
open url image1

Xu ZH, Zhang Y, Xia LQ, Xia XC, He ZH (2005) Genetic variation of starch properties in Chinese winter wheats. Acta Agronomica Sinica 31, 587–591.
|  CAS |
open url image1

Yamauchi H, Ito M, Nishio Z, Tabiki T, Kim SJ, Hashinoto N, Noda T, Takigawa S, Matsuura-Endo C, Takata K, Ohta K, Fukushima M, Miura H, Ism Z (2007) Effects of high-molecular-weight glutenin subunits on the texture of yellow alkaline noodles using near-isogenic lines. Food Science and Technology Research 13, 227–234.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Yun SH, Quail K, Moss R (1996) Physicochemical properties of Australian wheat flours for white salted noodles. Journal of Cereal Science 23, 181–189.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Zanetti S, Winzeler M, Feuillet C, Keller B, Messmer M (2001) Genetic analysis of bread-making quality in wheat and spelt. Plant Breeding 120, 13–19.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Zarco-Hernandez JA, Santiveri F, Michelena A, Peña RJ (2005) Durum wheat (Triticum turgidum L.) carrying the 1BL.1RS chromosomal translocation: agronomic performance and quality characteristics under Mediterranean conditions. European Journal of Agronomy 22, 33–43.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Zhang PP, He ZH, Chen DS, Zhang Y, Larroque OR, Xia XC (2007) Contribution of common wheat protein fractions to dough properties and quality of northern-style Chinese steamed bread. Journal of Cereal Science 46, 1–10.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Zhang Y, He ZH, Zhang AM, Ginkel M, Ye GY (2006) Pattern analysis on grain yield performance of Chinese and CIMMYT spring wheat cultivars sown in China and CIMMYT. Euphytica 147, 409–420.
| Crossref |
open url image1

Zhang Y, Quail K, Mugford DC, He ZH (2005) Milling quality and white salt noodle color of Chinese winter wheat cultivars. Cereal Chemistry 82, 633–638.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Zhang YL, Wu YP, Xiao YG, He ZH, Zhong Y, Yan J, Zhang Y, Xia XC, Ma CX (2009) QTL mapping for flour and noodle colour components and yellow pigment content in common wheat. Euphytica 165, 435–444.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1