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RESEARCH ARTICLE
Contents Vol 60(6)

QTL mapping for milling, gluten quality, and flour pasting properties in a recombinant inbred line population derived from a Chinese soft × hard wheat cross

Yelun Zhang A B , Yunpeng Wu A , Yonggui Xiao A , Jun Yan C , Yong Zhang A , Yan Zhang A , Chuanxi Ma B , Xianchun Xia A E and Zhonghu He A D E
+ Author Affiliations
- Author Affiliations

A Institute of Crop Science, National Wheat Improvement Centre/The National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing 100081, China.

B College of Agronomy, Anhui Agricultural University, Hefei, Anhui 230036, China.

C Cotton Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Huanghedadao, Anyang 455000, Henan Province, China.

D International Maize and Wheat Improvement Centre (CIMMYT) China Office, c/o CAAS, 12 Zhongguancun South Street, Beijing 100081, China.

E Corresponding authors. Email: xiaxianchun@caas.net.cn (Xianchun Xia) or zhhe@public3.bta.net.cn (Zhonghu He)

Crop and Pasture Science 60(6) 587-597 https://doi.org/10.1071/CP08392
Submitted: 3 November 2008  Accepted: 11 March 2009   Published: 12 June 2009

Abstract

Improvement of processing quality is important for various wheat-based end products. A recombinant inbred line (RIL) population derived from a cross between the Chinese wheat cvv. PH82-2 (hard) and Neixiang 188 (soft) was sown at 3 locations across two seasons to map quantitative trait loci (QTLs) for milling, gluten quality, flour pasting properties, and Chinese white salted noodle (CWSN) qualities. One hundred and eighty-eight microsatellite loci, one rye secalin marker Sec1, one STS marker YP7A, one CAPs marker for the Pinb-D1b allele, and four glutenin subunit markers were used to genotype the population and construct a linkage map for subsequent QTL analysis. In total, 53 QTLs for 16 quality parameters were mainly mapped to glutenin loci Glu-A3 (Glu-A3a:Glu-A3d), Glu-B1 (Bx7+By9:Bx14+By15), and Glu-D1 (Bx2+By12:Bx5+By10), and the grain hardness (Pinb-D1a:Pinb-D1b) locus. The-high-molecular weight glutenin subunits (HMW-GS) 5+10 at the Glu-D1 locus showed large effects on mixograph peak time (MPT), mixograph 8 min width (MTxW), and weakening slope (WS), accounting for 43.1%, 24.2%, and 39.7% of the phenotypic variance, respectively. In contrast, the 1RS (1B.1R translocation) showed large negative effects on MTxW, explaining 42.2% of the phenotypic variance. Two important QTLs were detected for Rapid Viscosity Analyzer (RVA) parameters: one for RVA final viscosity (RFV) near the 1RS and the other for RVA setback (RSb) associated with the Glu-B1 locus, explaining 21.6% and 12.3% of the phenotypic variance, respectively. Two QTLs for noodle adhesiveness were identified: one occurred on chromosome 1A and the other was associated with Glu-B1. Two QTLs for noodle springiness, one associated with the 1RS showing a negative effect on noodle quality and the other mapped to the Ha locus on chromosome 5DS, accounted for 9.4% and 8.1% of the phenotypic variance, respectively. In addition, the Ha locus also showed large effects on flower protein content (FPC), mixograph peak width (MPW), and RVA parameters, especially RVA pasting temperature (RPT), explaining 71.5% of the phenotypic variance.

Additional keywords: bread wheat, quantitative trait locus (QTL), quality parameters, molecular markers.


Acknowledgments

The authors are very grateful to Prof. R. A. McIntosh, Plant Breeding Institute, University of Sydney, Australia, for kindly reviewing this manuscript. This study was supported by the National Science Foundation of China (30771335 and 30830072), the National Basic Research Program (2009CB118300), and National 863 Programs (2006AA10Z1A7 and 2006AA100102).


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