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RESEARCH ARTICLE
Contents Vol 60(5)

Resistance to Subterranean clover mottle virus in Medicago truncatula and genetic mapping of a resistance locus

Muhammad Saqib A D , Simon R. Ellwood B , Roger A. C. Jones C and Michael G. K. Jones A
+ Author Affiliations
- Author Affiliations

A School of Biological Sciences and Biotechnology, Western Australian State Agricultural Biotechnology Centre, Murdoch University, Perth, WA 6150, Australia.

B ACNFP, School of Health Sciences, West Australian State Agricultural Biotechnology Centre, Murdoch University, Perth, WA 6150, Australia.

C Plant Pathology Section, Agricultural Research Western Australia, 3 Baron Hay Court, South Perth, WA 6151, Australia.

D Corresponding author. Email: m.saqib@murdoch.edu.au

Crop and Pasture Science 60(5) 480-489 https://doi.org/10.1071/CP08373
Submitted: 22 October 2008  Accepted: 2 March 2009   Published: 14 May 2009

Abstract

Subterranean clover mottle virus (SCMoV), which causes an important disease of annual clover pastures, was inoculated to the annual pasture legume Medicago truncatula, a model legume species used to help understand legume genome structure and function. Two hundred and nine accessions representing the core collection of M. truncatula were inoculated with infective sap containing SCMoV to determine their disease phenotypes. Forty-two of these accessions remained uninfected systemically and so were potentially resistant to the virus. Accession DZA315.16 developed a localised hypersensitive resistance reaction. In an F8 mapping population from a cross between the susceptible parent Jemalong 6/A17 and resistant accession DZA315.16, a total of 166 F8 recombinant inbred lines (RILs) were phenotyped for resistance and susceptibility to SCMoV. Resistant and susceptible lines showed parental phenotypic responses: 84 were susceptible and 82 were resistant, suggesting presence of a single resistance (R) gene. The phenotypic data were combined with genotypic data (76 polymorphic molecular markers) for this RIL population to provide a framework map. Genetic analysis located a single resistance locus termed RSCMoV1 on the long arm of chromosome 6. These results provide a basis for fine mapping the RSCMoV1 gene.

Additional keyword: virus resistance.


Acknowledgments

We thank Tracey Smith, Monica Kehoe, and Eva Gajda for technical support, John Fosu-Nyarko for help with symptom recording, Thierry Huguet (CNRS-INRA, France) for the RILs and genotyping data, Department of Agriculture and Food Western Australia for providing glasshouse and laboratory facilities, Murdoch University for an International Postgraduate Research Scholarship (to MS), and the Australian Research Council (DP 0771097) for additional financial support.


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