Infection of seed of subterranean clover and allied species by species of Fusarium

Abstract

Fusarium avenaceurn (Corda ex Fr.) Sacc. was shown for the first time to be carried in the hilum of subterranean clover (Trifolium Subterraneum L.) seed. Scanning electron microscopy and thin-section techniques showed that the fungus occurred only as dormant mycelium in parenchyma cells of funicle scar tissue. It emerged from these tissues after a 12 h incubation at 24¦C and in 48 h penetrated internal parts of the seed through the hilum fissure. After 21 days on moist blotting paper, seedlings grown from infected seed developed lesions on their roots similar to those of root-rot of subterranean clover in the field. Fusarium spp. were also detected in the hilum of seeds of white (T. repens L.) and strawberry (T. fragiferum L.) clover and barrel medic (M. truncatula L.). It was demonstrated experimentally that all parts of the burr, incl~tding the funicle, became infected with F. avenaceuni when subterranean clover plants grown from healthy seed in pasteurized soil buried their burrs in soil inoculated with the fungus. Use of optical brighteners failed to trace seed infection because the compounds, although absorbed by the pathogen in culture, were not translocated.