Colorimetric Aptasensor for Testosterone Detection Based on Aggregation of Gold Nanoparticles Induced by Cationic Surfactant
Chaoqiang Pan A , Jianmin Qiu A , Lumei Wang
A B , Zhiyu Yan A , Weiwen Huang A , Dongwei Zhang A , Xuejia Zhan A and Guoqing Shen A
+ Author Affiliations
- Author Affiliations
A School of Agriculture and Biology, Key Laboratory of Urban Agriculture, Ministry of Agriculture, Bor S. Luh Food Safety Research Center, Shanghai Jiao Tong University, Shanghai, 200240, China.
B Corresponding author. Email: zjuwlm@163.com
Australian Journal of Chemistry 74(4) 261-267 https://doi.org/10.1071/CH20237
Submitted: 29 July 2020 Accepted: 15 November 2020 Published: 9 December 2020
Abstract
This paper proposes a colorimetric aptasensor for the detection of testosterone (TES) in environmental water, using TES-specific aptamer (apT5) as a sensing probe, gold nanoparticles (AuNPs) as indicator, and hexadecyltrimethylammonium bromide (CTAB) as inducer, respectively. Based on competition between TES and CTAB for apT5, the aptamer can form an aptamer–TES complex, leaving CTAB free to aggregate AuNPs in the presence of TES. Dispersed and aggregated AuNPs have different absorption wavelengths and the signal of absorption intensity is associated with the concentration of TES, so TES can be detected quantitatively based on the signal absorption intensity. This sensitive aptasensor for TES detection has a wide linear range (R = 0.998) from 1.91–800 nM and a limit of detection (LOD) of 1.91 nM. In addition, this aptasensor has high selectivity over some interferents. The method detects TES in tap water samples with recoveries in the range of 98.9–102.6 % (RSD ≤ 7.35 %). This biosensor presents a good and potential application to rapidly detect TES in actual environmental water samples.
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