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RESEARCH ARTICLE
Contents Vol 73(3)

The Effect of Charge on Melittin-Induced Changes in Membrane Structure and Morphology

Tzong-Hsien Lee A B , Kristopher Hall A B and Marie-Isabel Aguilar A C
+ Author Affiliations
- Author Affiliations

A Department of Biochemistry and Molecular Biology, Monash University, Clayton, Vic. 3800, Australia.

B These authors contributed equally to this work.

C Corresponding author. Email: mibel.aguilar@monash.edu

Australian Journal of Chemistry 73(3) 195-201 https://doi.org/10.1071/CH19500
Submitted: 4 October 2019  Accepted: 4 November 2019   Published: 20 December 2019

Abstract

The binding of melittin to a range of phospholipid bilayers was studied using dual polarisation interferometry and atomic force microscopy. The phospholipid model membranes included zwitterionic dimyristylphosphatidylcholine (DMPC), together with mixtures of DMPC/dimyristylphosphatidylglycerol (DMPG) and DMPC/DMPG/cholesterol. Melittin caused significant disruption on all bilayers, but differences in morphological changes during binding were different on each membrane. Overall, the results demonstrate that the process of membrane disruption follows distinct structural changes for different lipid mixtures irrespective of the strength of binding to the membrane surface.


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