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RESEARCH ARTICLE (Open Access)
Contents Vol 67(6)

Effects of Different Phases of Cigarette Smoke on Lipid Peroxidation and Membrane Structure in Liposomes

Nadezhda P. Palmina A , Elena L. Maltseva A , Tatyana E. Chasovskaya A , Valery V. Kasparov A , Nataliya G. Bogdanova A , Valery A. Menshov A and Aleksei V. Trofimov A B
+ Author Affiliations
- Author Affiliations

A Emanuel Institute of Biochemical Physics, RAS, Kosygin str. 4, 119334 Moscow, Russia.

B Corresponding author. Email: avt_2003@mail.ru

Australian Journal of Chemistry 67(6) 858-866 https://doi.org/10.1071/CH13663
Submitted: 30 November 2013  Accepted: 21 January 2014   Published: 24 February 2014

Abstract

This paper discloses for the first time the effects of the gas phase (GP) and the tar of cigarette smoke on lipid peroxidation (LPO) and on the structure of different lipid regions in liposomes. The LPO development was analysed in terms of the total unsaturation of lipids (double-bond, DB, content) and the formation of dienic conjugates (DC), ketodienes (KD), and malonic dialdehyde (MDA). As expected, the exposure of liposomes to either the GP or the tar led to a significant decrease in the DB content. However, the formation of oxidation products revealed different dynamics: MDA generation was inhibited, while the formation of DC and KD increased during the first few hours of the LPO development followed by its inhibition. The smoke constituents exhibited opposite effects on the structure of the lipid bilayer of liposomes: the GP markedly enhanced the microviscosity of liposomal membranes, whereas the tar caused a drastic lowering of microviscosity.


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