Microwave-Assisted Synthesis of Arene Ruthenium(ii) Complex as Apoptosis Inducer of A549 Cells

Qiong Wu; Jian Wu; Wen-Jie Mei; Qi Wang; Zhao Zhang; Xiao-Hui Wu; Fen-Yong Sun; Wei-Li Wu; Yan-Hua Chen; Xiao-Ying Hu; Yun-Yi Tao

doi:10.1071/CH13269

RESEARCH ARTICLE

Microwave-Assisted Synthesis of Arene Ruthenium(ii) Complex as Apoptosis Inducer of A549 Cells

Qiong Wu ^A , Jian Wu ^A , Wen-Jie Mei ^A ^C , Qi Wang ^A , Zhao Zhang ^A , Xiao-Hui Wu ^A , Fen-Yong Sun ^B ^C , Wei-Li Wu ^A , Yan-Hua Chen ^A , Xiao-Ying Hu ^A and Yun-Yi Tao ^A

+ Author Affiliations

- Author Affiliations

^A School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, 510006, China.

^B Department of Clinical Laboratory Medicine, Shanghai Tenth People’s Hospital of Tongji University, Shanghai, 200072, China.

^C Corresponding authors. Email: wenjiemei@126.com; sunfenyong@263.net

Australian Journal of Chemistry 66(11) 1422-1427 https://doi.org/10.1071/CH13269
Submitted: 28 May 2013 Accepted: 25 July 2013 Published: 13 September 2013

Abstract

An arene ruthenium(ii) complex coordinated with 2-(2-chlorophenyl)-1H-imidazo[4,5-f][1,10]phenanthroline, [(η⁶-C₆H₆)Ru(o-ClPIP)Cl]Cl (1), has been prepared by using microwave-assisted synthesis technology. The anti-tumour activity of this complex against various tumour cells has been evaluated by MTT assay and the results show that complex 1 exhibits selective inhibitory activity against the growth of human lung adenocarcinoma A549 cells with IC₅₀ = 31.58 μM. Further studies by flow cytometric analysis showed that apoptosis of A549 cells was observed when dealt with complex 1. Furthermore, complex 1 exhibits excellent binding affinity with DNA molecules which was confirmed by spectroscopy methods, as well viscosity and melting point experiments. As a result, the conformation of DNA molecules was disturbed by complex 1.

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Microwave-Assisted Synthesis of Arene Ruthenium(ii) Complex as Apoptosis Inducer of A549 Cells

Abstract

References

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