Matrix Metalloproteinase Biosensor Based on a Porous Silicon Reflector
Fransiska S. H. Krismastuti A , Stephanie Pace A , Elizabeth Melville A , Allison Cowin A , Tim R. Dargaville B and Nicolas H. Voelcker A C
+ Author Affiliations
- Author Affiliations
A Mawson Institute, University of South Australia, Mawson Lakes, SA 5095, Australia.
B Tissue Repair and Regeneration Program, Queensland University of Technology, Institute of Health and Biomedical Innovation, Kelvin Grove, Qld 4059, Australia.
C Corresponding author. Email: nico.voelcker@unisa.edu.au
Australian Journal of Chemistry 66(11) 1428-1434 https://doi.org/10.1071/CH13352
Submitted: 5 July 2013 Accepted: 30 July 2013 Published: 4 September 2013
Abstract
Matrix metalloproteinases (MMP) are proteolytic enzymes important to wound healing. In non-healing wounds, it has been suggested that MMP levels become dysfunctional, hence it is of great interest to develop sensors to detect MMP biomarkers. This study presents the development of a label-free optical MMP biosensor based on a functionalised porous silicon (pSi) thin film. The biosensor is fabricated by immobilising a peptidomimetic MMP inhibitor in the porous layer using hydrosilylation followed by amide coupling. The binding of MMP to the immobilised inhibitor translates into a change of effective optical thickness over time. We investigated the effect of surface functionalisation on the stability of the pSi surface and evaluated sensing performance. We successfully demonstrated MMP detection in buffer solution and human wound fluid at physiologically relevant concentrations. This biosensor may find application as a point-of-care device that is prognostic of the healing trajectory of chronic wounds.
References
[1] A. J. M. Boulton, L. Vileikyte, G. Ragnarson-Tennvall, J. Apelqvist, Lancet 2005, 366, 1719.| Crossref | GoogleScholarGoogle Scholar |
[2] T. Velnar, T. Bailey, V. Smrkolj, J. Int. Med. Res. 2009, 37, 1528.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXht1Oqsbs%3D&md5=f5afa54d3ede50937cef2ba70d812899CAS | 19930861PubMed |
[3] R. Moseley, J. R. Hilton, R. J. Waddington, K. G. Harding, P. Stephens, D. W. Thomas, Wound Repair Regen. 2004, 12, 419.
| Crossref | GoogleScholarGoogle Scholar | 15260807PubMed |
[4] R. Moseley, J. E. Stewart, P. Stephens, R. J. Waddington, D. W. Thomas, Br. J. Dermatol. 2004, 150, 401.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXjs1aiu78%3D&md5=2c0e5ed0a289dc95fa279524b5e5586eCAS | 15030321PubMed |
[5] S. A. Eming, M. Koch, A. Krieger, B. Brachvogel, S. Kreft, L. Bruckner-Tuderman, T. Krieg, J. D. Shannon, J. W. Fox, J. Proteome Res. 2010, 9, 4758.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXhtVSju7fE&md5=c496c689bb723d2631f1df5bfa52ad3aCAS | 20666496PubMed |
[6] H. Brem, O. Stojadinovic, R. F. Diegelmann, H. Entero, B. Lee, I. Pastar, M. Golinko, H. Rosenberg, M. Tomic-Canic, Mol. Med. 2007, 13, 30.
| Crossref | GoogleScholarGoogle Scholar | 17515955PubMed |
[7] C. K. Sen, G. M. Gordillo, S. Roy, R. Kirsner, L. Lambert, T. K. Hunt, F. Gottrup, G. C. Gurtner, M. T. Longaker, Wound Repair Regen 2009, 17, 763.
| Crossref | GoogleScholarGoogle Scholar | 19903300PubMed |
[8] T. R. Dargaville, B. L. Farrugia, J. A. Broadbent, S. Pace, Z. Upton, N. H. Voelcker, Biosens. Bioelectron. 2013, 41, 30.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XhsVyhsb7O&md5=ca2c79420e50a7721700b8e6572bdf7cCAS | 23058663PubMed |
[9] K. Harding, Principles of Best Practice: Diagnostics and Wounds. A Concensus Document. 2008 (Medical Education Partnership (MEP) Ltd: London).
[10] A. L. Clutterbuck, P. Harris, D. Allaway, A. Mobasheri, Vet. J. 2010, 183, 27.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXhsFyntbjE&md5=034aa857287d608ae3496ff8db2f5045CAS | 19022687PubMed |
[11] F.-X. Gomis-Ruth, K. Maskos, M. Betz, A. Bergner, R. Huber, K. Suzuki, G. P. Bourenkov, H. Bartunik, W. Bode, Nature 1997, 389, 77.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2sXlvVCksrs%3D&md5=cd030b957127b146ce7735ae249c8aefCAS | 9288970PubMed |
[12] J. F. Woessner, FASEB J. 1991, 5, 2145.
| 1:CAS:528:DyaK3MXisVeiurs%3D&md5=0e091c7606178abcfd92e168dc37780eCAS | 1850705PubMed |
[13] B. Beekman, J. W. Drijfhout, W. Bloemhoff, H. K. Ronday, P. P. Tak, J. M. te Koppele, FEBS Lett. 1996, 390, 221.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK28XkvVCqsr0%3D&md5=2aa2fc82edcc58e61b3a338766b59762CAS | 8706864PubMed |
[14] W. Bode, C. Fernandez-Catalan, H. Tschesche, F. Grams, H. Nagase, K. Maskos, Cell. Mol. Life Sci. 1999, 55, 639.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1MXjtlWlurw%3D&md5=f04eac4d58f05e469563fd0293de8630CAS | 10357232PubMed |
[15] R. Visse, H. Nagase, Circ. Res. 2003, 92, 827.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXjtFWqu7c%3D&md5=592cdcb4f52eaba8bf7805549177da61CAS | 12730128PubMed |
[16] Q.-X. A. Sang, Y. Jin, R. G. Newcomer, S. C. Monroe, X. Fang, D. R. Hurst, S. Lee, Q. Cao, M. A. Schwartz, Curr. Top. Med. Chem. 2006, 6, 289.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XmtVahsLc%3D&md5=473a4d795ce227290fbc0c8278ac8edfCAS |
[17] Y. Cao, T. I. Croll, S. C. Rizzi, G. K. Shooter, H. Edwards, K. Finlayson, Z. Upton, T. R. Dargaville, J. Biomed. Mater. Res. 2011, 96A, 663.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXhsFejuro%3D&md5=9b89c7ed1c087793fb101ce17a183f7bCAS |
[18] G. S. Schultz, B. A. Mast, Primary Intention 1999, 7.
[19] G. B. Fields, Using Fluorogenic Peptide Substrates to Assay Matrix Metalloproteinases. In Matrix Metalloproteinase Protocols (Ed. I. M. Clark) 2010, p. 393 (Springer Protocols: Heidelberg)
[20] S.-H. Jung, D.-H. Kong, J. H. Park, S.-T. Lee, J. Hyun, Y.-M. Kim, K.-S. Ha, Analyst 2010, 135, 1050.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXlt1Chsrc%3D&md5=d9a665ada5cfa3d6b141b4ef80f9068eCAS | 20419256PubMed |
[21] U. Pieper-Fürst, U. Kleuser, W. F. M. Stocklein, A. Warsinke, F. W. Scheller, Anal. Biochem. 2004, 332, 160.
| Crossref | GoogleScholarGoogle Scholar | 15301961PubMed |
[22] M. Martin, C. T. Bendiab, L. Massif, G. Palestino, V. Agarwal, F. Cuisinier, C. Gergely, Phys. Status Solidi 2010, C, 1.
[23] B. S. Munge, J. Fisher, L. N. Millord, C. E. Krause, R. S. Dowd, J. F. Rusling, Analyst 2010, 135, 1345.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXmsFSlsLg%3D&md5=d475fb262f1dad6086b639be3a984152CAS | 20358056PubMed |
[24] V. S.-Y. Lin, K. Motesharei, K. Dancil, M. J. Sailor, M. R. Ghadiri, Science 1997, 278, 840.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2sXmvFOqsb4%3D&md5=3e79977861eca46bb8c8e5bd2723eb5cCAS |
[25] A. Jane, R. Dronov, A. Hodges, N. H. Voelcker, Trends Biotechnol. 2009, 27, 230.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXjvVahtrY%3D&md5=56b77ba14e95920769f62dc70fcbbde3CAS | 19251329PubMed |
[26] J. M. Buriak, Chem. Rev. 2002, 102, 1271.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XhslamtLg%3D&md5=c4f07a66e1d85624eb387c830b5839eaCAS | 11996538PubMed |
[27] J. M. Buriak, M. P. Stewart, T. W. Geders, M. J. Allen, H. C. Choi, J. Smith, D. Raftery, L. T. Canham, J. Am. Chem. Soc. 1999, 121, 11491.
| 1:CAS:528:DyaK1MXnsFOmtr0%3D&md5=282e50a42588bdbd201f14cb0d22b750CAS |
[28] M. Latterich, J. Corbeil, Proteome Sci. 2008, 6, 31.
| Crossref | GoogleScholarGoogle Scholar | 18983648PubMed |
[29] C. Steinem, A. Janshoff, V. S.-Y. Lin, N. H. Voelcker, M. R. Ghadiri, Tetrahedron 2004, 60, 11259.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXpt1WktLs%3D&md5=cbe2f411927b516400f16b74f1dce8cdCAS |
[30] N. H. Voelcker, I. Alfonso, M. R. Ghadiri, ChemBioChem 2008, 9, 1776.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXpsVyns7o%3D&md5=70f60c1c140e851fbce50bd4773c4adeCAS | 18576449PubMed |
[31] K. A. Kilian, T. Bocking, J. J. Gooding, Chem. Commun. 2009, 630.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXht1yjsLc%3D&md5=4417df9644bba23f7e43d05c3fae6187CAS |
[32] H. Ouyang, M. Christophersen, P. M. Fauchet, Phys. Status Solidi A 2005, 202, 1396.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXmtVensr8%3D&md5=13c1a5e29dafe3acc3b0ca0da1549db0CAS |
[33] B. L. Miller, Nano-structured Silicon Optical Sensors. In Optical Guided-wave Chemical and Biosensors II (Eds M. Zourob, A. Lakhtakia) 2010. p. 3 (Springer: Berlin)
[34] S. Kim, B. Cho, H. Sohn, Nanoscale Res. Lett. 2012, 7, 527.
| Crossref | GoogleScholarGoogle Scholar | 23009146PubMed |
[35] S. Pace, R. B. Vasani, F. Cunin, N. H. Voelcker, New J. Chem. 2013, 37, 228.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XhvVert7nJ&md5=d4f831bcacf279c3716d57c7efbc254eCAS |
[36] S. Iyer, R. Visse, H. Nagase, K. R. Archarya, J. Mol. Biol. 2006, 362, 78.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XovVSrtbw%3D&md5=b530f3514eff220a2f5be0886d7f12a6CAS | 16890240PubMed |
[37] M. Arroyo-Hernandez, R. J. Martin-Palma, J. Perez-Rigueiro, J. P. Garcia-Ruiz, J. L. Garcia-Fierro, J. M. Martinez-Duart, Mater. Sci. Eng. C 2003, 23, 697.
| Crossref | GoogleScholarGoogle Scholar |
[38] B. Guan, A. Magenau, K. A. Kilian, S. Ciampi, K. Gaus, P. J. Reece, J. J. Gooding, Faraday Discuss. 2011, 149, 301.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXhs1Sruw%3D%3D&md5=1f5a624a4c007c2a14b9f5c401716633CAS | 21413188PubMed |
[39] S. Sam, L. Touahir, J. Salvador Andresa, P. Allongue, J. N. Chazalviel, A. C. Gouget-Laemmel, C. Henry de Villenueve, A. Moraillon, F. Ozanam, N. Gabouze, S. Djebbar, Langmuir 2010, 26, 809.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXhtVyhs7vP&md5=adb9cab0b19b5be1cbe76b20fe62d7d9CAS | 19725548PubMed |
[40] A. Janshoff, K.-P. S. Dancil, C. Steinem, D. P. Greiner, V. S.-Y. Lin, C. Gurtner, K. Motesharei, M. J. Sailor, M. R. Ghadiri, J. Am. Chem. Soc. 1998, 120, 12108.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1cXntVKgurg%3D&md5=61a74c133928fa7bcacc5196a2563810CAS |
[41] M. J. Sweetman, C. J. Shearer, J. G. Shapter, N. H. Voelcker, Langmuir 2011, 27, 9497.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXotlGit7g%3D&md5=ec02c2ca1f542c9fc179ef4f4c51debdCAS | 21678982PubMed |
[42] R. Boukherroub, J. T. C. Wojtyk, D. D. M. Wayner, D. J. Lockwood, J. Electrochem. Soc. 2002, 149, H59.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XhtlKnsLk%3D&md5=76aada32b04d5baf9b38fcc4fe72f14fCAS |
[43] P. Ziemiański, J. Misiewicz, Phys. Status Solidi A 1995, 149, K47.
| Crossref | GoogleScholarGoogle Scholar |
[44] I. N. Lees, H. Lin, C. A. Canaria, C. Gurtner, M. J. Sailor, G. M. Miskelly, Langmuir 2003, 19, 9812.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXosVCrt7s%3D&md5=f8ecaf2f58f262d8a19966e49c8a5c42CAS |
[45] C. Pacholski, M. Sartor, M. J. Sailor, F. Cunin, G. M. Miskelly, J. Am. Chem. Soc. 2005, 127, 11636.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXms1Kmsr0%3D&md5=bcc3ff393efabfd4fdb24e92f0fd2e50CAS | 16104739PubMed |
[46] N. J. Trengove, M. C. Stacey, S. Macauley, N. Bennett, J. Gibson, F. Burslem, G. Murphy, G. Schultz, Wound Repair Regen. 1999, 7, 442.
| Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD3c%2FpvVyruw%3D%3D&md5=446ce562dd40e4cbe43317803a633985CAS | 10633003PubMed |
[47] A. Wysocki, L. Staiano-Coico, F. Grinnell, J. Invest. Dermatol. 1993, 101, 64.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3sXmsVOisLs%3D&md5=103b3486bcfd062a6f1aa3430d2d253fCAS | 8392530PubMed |
[48] R. B. Vasani, S. J. P. McInnes, M. A. Cole, A. M. M. Jani, A. V. Ellis, N. H. Voelcker, Langmuir 2011, 27, 7843.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXmtlymtbY%3D&md5=fa81a0f602a7234dc8d54701eec60214CAS | 21604788PubMed |
