Detection of DNA Damage Induced by Hydroquinone and Catechol Using an Electrochemical DNA Biosensor

Chuanhu Wang; Jun Zhao; Dapeng Zhang; Zhousheng Yang

doi:10.1071/CH08229

RESEARCH ARTICLE

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Detection of DNA Damage Induced by Hydroquinone and Catechol Using an Electrochemical DNA Biosensor

Chuanhu Wang ^A ^B , Jun Zhao ^A , Dapeng Zhang ^A and Zhousheng Yang ^A ^C

+ Author Affiliations

- Author Affiliations

^A School of Chemistry and Materials Science, Anhui Key Laboratory of Functional Molecular Solids, Anhui Normal University, Wuhu 241000, China.

^B Science Department, Bengbu College, Bengbu 233000, China.

^C Corresponding author. Email: yzhoushe@mail.ahnu.edu.cn

Australian Journal of Chemistry 62(9) 1181-1184 https://doi.org/10.1071/CH08229
Submitted: 1 June 2008 Accepted: 6 May 2009 Published: 17 September 2009

Abstract

DNA damage induced by hydroquinone and catechol was detected by an electrochemical method. Calf thymus DNA was immobilized onto the surface of a pretreated glassy carbon electrode (GCE(ox)) to form a DNA/GCE(ox) modified electrode. Then the DNA/GCE(ox) was incubated in acetate buffer solution containing hydroquinone or catechol at a constant potential for the desired time. Differential pulse voltammetric experiments were then performed. The anodic peaks corresponding to the oxidation of guanisine and adenosine on the electrode could be observed on voltammetric curves. The experimental results showed that DNA damage could be detected using electrochemical DNA biosensors. The extent of DNA damage could be electrochemically recognized via the change of the anodic peak current. DNA damage induced by hydroquinone was greater than that by catechol. The response conditions were optimized with respect to DNA concentration, pH, ionic strength, and other variables.

Acknowledgements

We thank the National Natural Science Foundation of China (grant no. 20775002) for financial support. The work was supported by the Program for Innovative Research Team in Anhui Normal University.

References

Detection of DNA Damage Induced by Hydroquinone and Catechol Using an Electrochemical DNA Biosensor

Abstract

Acknowledgements

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