How temperature, substrate, and stratification periods influence Ilex paraguariensis embryonic development
Manoela Mendes Duarte
A B * , Letícia Siqueira Walter A , Mônica Moreno Gabira A , Cléberson Porath A , Elisa Serra Negra Vieira B , Antonio Carlos Nogueira A , Ivar Wendling B and Dagma Kratz A
+ Author Affiliations
- Author Affiliations
A Universidade Federal do Paraná, Departamento de Ciências Florestais e da Madeira, Curitiba, Paraná, Brazil.
B Embrapa Florestas – Empresa Brasileira de Pesquisa Agropecuária, Colombo, Paraná, Brazil.
Australian Journal of Botany 71(5) 269-273 https://doi.org/10.1071/BT23010
Submitted: 28 January 2023 Accepted: 30 May 2023 Published: 16 June 2023
© 2023 The Author(s) (or their employer(s)). Published by CSIRO Publishing
Abstract
Aiming to develop a new stratification protocol for yerba mate seeds, we evaluated how different combinations of temperature, substrate, and period influence the embryonic development of the species, compared to fresh seeds and conventionally stratified seeds. We measured the seeds’ moisture content and performed a tetrazolium test to evaluate embryo viability and embryonic development. Seeds’ initial moisture content was 10.1% and reached 40.6% after stratification. We observed more embryos in advanced development stages after stratification in all treatments, indicating that the protocols used were adequate for the species and have a great potential to be explored for yerba mate seedling.
Keywords: seed development, seed dormancy, seed germination, seed viability, seminal propagation, stratification protocol, tetrazolium test, yerba mate.
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