Evidence of inter-sectional chloroplast capture in Corymbia among sections Torellianae and Maculatae
Adam Healey A B , David J. Lee C , Agnelo Furtado A and Robert J. Henry A D
+ Author Affiliations
- Author Affiliations
A Queensland Alliance for Agriculture and Food Innovation, University of Queensland, Brisbane, Qld 4072, Australia.
B Present address: HudsonAlpha Institute for Biotechnology, 601 Genome Way, Huntsville, AL 35806, USA.
C University of the Sunshine Coast, Locked Bag 4, Maroochydore DC, Qld 4558, Australia.
D Corresponding author. Email: robert.henry@uq.edu.au
Australian Journal of Botany 66(5) 369-378 https://doi.org/10.1071/BT18028
Submitted: 7 February 2018 Accepted: 10 July 2018 Published: 30 August 2018
Abstract
Chloroplast capture through hybridisation and introgression is well described within Eucalyptus. Despite the propensity of the Corymbia genus (eucalypts) to form hybrids from wide crosses, description of chloroplast capture in Corymbia has, until recently, been limited. In this study our aim was to investigate evidence of intersectional chloroplast capture between sections Torellianae and Maculatae. Using whole-genome next-generation sequencing data, the complete chloroplast genomes were assembled from four Corymbia taxa: Corymbia citriodora subspecies citriodora (Hook.) K.D.Hill & L.A.S.Johnson, Corymbia citriodora subspecies variegata (F.Muell.) A.R.Bean & M.W.McDonald, Corymbia henryi (S.T.Blake) K.D.Hill & L.A.S.Johnson, and Corymbia torelliana (F.Muell.) K.D.Hill & L.A.S.Johnson, represented by eight genotypes. Phylogenetic analysis and comparison among Corymbia chloroplast genomes and nuclear external transcribed spacer (ETS) sequences revealed chloroplast capture among Corymbia species across distinct sections Torellianae and Maculatae within subgenus Blakella. Reticulate evolution, along with Eucalyptus, likely extends into Corymbia as evidenced by incongruent plastid and nuclear phylogenetic trees, suggestive of its importance of hybridisation and introgression during the evolution of eucalypts.
Additional keywords: Eucalyptus, introgression, phylogeny, reticulate evolution, whole chloroplast.
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