Embryo induction and plant regeneration of Callerya speciosa (Fabaceae) through anther culture
Bilan Huang A , Li Xu A , Kelie Li A , Yunlu Fu A and Zhiying Li A BA Institute of Tropical Crops Genetic Resources, Chinese Academy of Tropical Agricultural Sciences, Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture. Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation, Danzhou, Hainan 571737, China.
B Corresponding author. Email: xllizhiying@vip.163.com
Australian Journal of Botany 65(1) 80-84 https://doi.org/10.1071/BT16112
Submitted: 1 June 2016 Accepted: 16 December 2016 Published: 24 January 2017
Abstract
An in vitro protocol for Callerya speciosa (Champ.) Schot regeneration through embryogenesis was developed using the anthers as the explants. The late uninucleate stage of the microspore was optimal for the anther culture of C. speciosa. Embryonic callus was induced on a MS basal medium supplemented with 4.4 µM 6-benzylaminopurine (BA) and 9.04 µM 2,4-dichlorophenoxyacetic acid (2,4-D). Embryos were obtained on MS medium supplemented with 2.2 µM BA and 0.5 µM naphthaleneacetic acid (NAA). The highest percentage (16.7%) of embryos was achieved using the culture medium MS + 0.25 µM NAA + 1.1 µM BA. The highest percentage of embryos that developed into plants was 18.3%. However, haploid plants were not observed, which may have been due to the collection of the calli from the anther wall. The results presented here demonstrate the establishment of a highly efficient and rapid system for regenerating C. speciosa using anther cultures.
Additional keywords: anther, C. speciosa (Fabaceae), embryogenesis, plant regeneration.
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