Autochthonous Streptomyces regulate the metabolism of seedlings of Araucaria angustifolia (Coniferales) during root colonisation
F. R. Dalmas A , T. C. B. Pereira B , M. R. Bogo B and L. V. Astarita A C
+ Author Affiliations
- Author Affiliations
A Laboratory of Plant Biotechnology, Bioscience Institute, Pontifícia Universidade Católica do Rio Grande do Sul, Ipiranga Avenue, 6681, Building 12A, CEP: 90619-900, Porto Alegre, RS, Brazil.
B Laboratory of Genomics and Molecular Biology, Bioscience Institute, Pontifícia Universidade Católica do Rio Grande do Sul, Ipiranga Avenue, 6681, Building 12A, CEP: 90619-900, Porto Alegre, RS, Brazil.
C Corresponding author. Email: astarita@pucrs.br
Australian Journal of Botany 59(2) 118-125 https://doi.org/10.1071/BT10175
Submitted: 13 July 2010 Accepted: 10 January 2011 Published: 28 March 2011
Abstract
Araucaria angustifolia (Bertol.) Kuntze, known as Brazilian pine, is an endangered species of great ecological and economic importance. This species grows slowly and unevenly, with high mortality in commercial plantations. Streptomyces is a genus of soil microorganisms that may have a beneficial effect on plant growth. This study evaluated the effect of three autochthonous Streptomyces spp. isolates (PM1, PM4 and PM9) on the initial metabolism and development of A. angustifolia seedlings. The enzymatic activity of phenylalanine ammonia-lyase, polyphenol oxidase and peroxidase, and the levels of phenolic compounds, flavonoids and chlorophyll were determined in extracts from roots and leaves of the seedlings. Assays were carried out 1, 3 and 9 days after the roots were inoculated with each isolate. Length and fresh mass of shoots and roots as well as the volume and density of roots were evaluated at 100 days after seedling inoculation. All the Streptomyces spp. showed rhizospheric competence and produced auxin. The activities of polyphenol oxidase and peroxidase exhibited a tissue-temporal regulation in the presence of the isolates. Levels of phenolics, flavonoids and chlorophylls did not change in the period analysed. The root system of seedlings inoculated with all isolates was shorter and denser, with a small volume. The PM9 isolate promoted shoot growth and affected plant metabolism, proving to be a promising rhizobacterium with a plant growth-promoting rhizobacteria role.
References
Bais HP, Weir TL, Perry LG, Gilroy S, Vivanco JM (2006) The role of root exudates in rhizosphere interactions with plants and other organisms. Annual Review of Plant Biology 57, 233–266.| The role of root exudates in rhizosphere interactions with plants and other organisms.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XosVKhtr8%3D&md5=90489f470d2855ee5306add9a379c7ccCAS | 16669762PubMed |
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248–254.
| A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaE28XksVehtrY%3D&md5=0a795ded818757007102fefb54d36e29CAS | 942051PubMed |
Butland SL, Chow ML, Ellis BE (1998) A diverse family of phenylalanine ammonia-lyase genes expressed in pine trees and cell cultures. Plant Molecular Biology 37, 15–24.
| A diverse family of phenylalanine ammonia-lyase genes expressed in pine trees and cell cultures.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1cXjtlajt7o%3D&md5=d794b50742d6521434bee61ee16929e8CAS | 9620261PubMed |
Duijff BJ, Gianinazzi-pearson V, Lemanceau P (1997) Involvement of the outer membrane lipopolysaccharides in the endophytic colonization of tomato roots by biocontrole Pseudomonas fluorescens strain WCS417r. New Phytologist 135, 325–334.
| Involvement of the outer membrane lipopolysaccharides in the endophytic colonization of tomato roots by biocontrole Pseudomonas fluorescens strain WCS417r.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2sXis12itLY%3D&md5=665caac9f62a11a21ea4d90fe4669b8aCAS |
Errakhi R, Bouteau F, Lebrihi A, Barakate M (2007) Evidences of biological control capacities of Streptomyces spp. against Sclerotium rolfsii responsible for damping-off disease in sugar beet (Beta vulgaris L.). World Journal of Microbiology & Biotechnology 23, 1503–1509.
| Evidences of biological control capacities of Streptomyces spp. against Sclerotium rolfsii responsible for damping-off disease in sugar beet (Beta vulgaris L.).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXhtFSltr3F&md5=753c80564d72b320e142c73a2103901cCAS |
Frey-Klett P, Garbaye J, Tarkka M (2007) The mycorrhiza helper bacteria revisited. New Phytologist 176, 22–36.
| The mycorrhiza helper bacteria revisited.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD2sngvF2jsg%3D%3D&md5=37d3c557ed5b4ae7efbb94a4e99f38c8CAS | 17803639PubMed |
Guerra MP, Silveira V, Reis MS, Schneider L (2003) Exploração, manejo e conservação da araucária (Araucaria angustifolia). In ‘Sustentável Mata Atlântica: a exploração de seus recursos florestais’. (Eds LL Simões, CF Lino) pp. 85–101. (Senac Editora: São Paulo)
Haas D, Keel C, Reimmann C (2002) Signal transduction in plant-beneficial rhizobacteria with biocontrole properties. Antonie van Leeuwenhoek 81, 385–395.
| Signal transduction in plant-beneficial rhizobacteria with biocontrole properties.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38Xns1SgtLg%3D&md5=4aefda85dbd0114d285f20bb937b909bCAS | 12448737PubMed |
Hayakawa M, Nonomura H (1989) A new method for the intensive isolation of actinomycetes from soil. Actinomycetologica 3, 95–104.
| A new method for the intensive isolation of actinomycetes from soil.Crossref | GoogleScholarGoogle Scholar |
Horinouchi S (2007) Mining and polishing of the treasure trove in the bacterial genus Streptomyces. Bioscience, Biotechnology, and Biochemistry 71, 283–299.
| Mining and polishing of the treasure trove in the bacterial genus Streptomyces.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXivV2itr4%3D&md5=b618a66efc23dbfeffb17e11e842688fCAS | 17284841PubMed |
Hudgins JW, Franceschi VR (2004) Methyl jasmonate-induced ethylene production is responsible for conifer phloem defense responses and reprogramming of stem defense responses and reprogramming of stem cambial zone for traumatic resis duct formation. Plant Physiology 135, 2134–2149.
| Methyl jasmonate-induced ethylene production is responsible for conifer phloem defense responses and reprogramming of stem defense responses and reprogramming of stem cambial zone for traumatic resis duct formation.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXnt1Ggs7g%3D&md5=78bc588f9f39b51822ed5b19faa76a1cCAS | 15299142PubMed |
Krokene P, Nagy NE, Solheim H (2008) Methyl jasmonate and oxalic acid treatment of Norway spruce: anatomically based defense responses and increased resistance against fungal infection. Tree Physiology 28, 29–35.
Latunde-Dada AO, Lucas JA (2001) The plant defence activator acibenzolar-S-methyl primes cowpea [Vigna unguiculata (L.) Walp.] seedlings for rapid induction of resistance. Physiological and Molecular Plant Pathology 58, 199–208.
| The plant defence activator acibenzolar-S-methyl primes cowpea [Vigna unguiculata (L.) Walp.] seedlings for rapid induction of resistance.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXltFanurs%3D&md5=3dd13fec2661f11adcaa20e1df39d4f8CAS |
Lehr NA, Schrey SD, Bauer R, Hampp R, Tarkka MT (2007) Suppression of plant defence response by a mycorrhiza helper bacterium. New Phytologist 174, 892–903.
| Suppression of plant defence response by a mycorrhiza helper bacterium.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXnt1amtbc%3D&md5=017fe89879f10cef11101199f773069bCAS | 17504470PubMed |
Lehr NA, Schrey SD, Hampp R, Tarkka MT (2008) Root inoculation with a forest soil streptomycete leads to locally and systemically increased resistance against phytopathogens in Norway spruce. New Phytologist 177, 965–976.
| Root inoculation with a forest soil streptomycete leads to locally and systemically increased resistance against phytopathogens in Norway spruce.Crossref | GoogleScholarGoogle Scholar | 18086220PubMed |
Long HH, Schmidt DD, Baldwin IT (2008) Native bacterial endophytes promote host growth in a species-specific manner; phytohormone manipulations do not result in common growth responses. PLoS ONE 3, e2702
| Native bacterial endophytes promote host growth in a species-specific manner; phytohormone manipulations do not result in common growth responses.Crossref | GoogleScholarGoogle Scholar | 18628963PubMed |
MMA (Ministério do Meio Ambiente) (2002) ‘Biodiversidade Brasileira. Avaliação e identificação de áreas e ações prioritárias para conservação, utilização sustentável e repartição dos benefícios da biodiversidade nos biomas brasileiros.’ (Ministério do Meio Ambiente/Secretaria de Biodiversidade e Florestas Press: Brasília, DF, Brazil). Available at www.mma.gov.br/estruturas/chm/_arquivos/Bio5.pdf
Moreira-Souza M, Cardoso EJBN (2002) Dependência micorrízica de Araucaria angustifolia (Bert.) O. Ktze. sob doses de fósforo. Revista Brasileira de Ciencia do Solo 26, 905–912.
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15, 473–497.
| A revised medium for rapid growth and bioassays with tobacco tissue cultures.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaF3sXksFKm&md5=a958e803d17732f6cdd7c8ffe2983c5fCAS |
Nagy NE, Fossdal CG, Dalen LS, Lönneborg A, Heldal I, Johnsen O (2004) Effects of Rhizoctonia infection and drought on peroxidase and chitinase activity in Norway spruce (Picea abies). Physiologia Plantarum 120, 465–473.
| Effects of Rhizoctonia infection and drought on peroxidase and chitinase activity in Norway spruce (Picea abies).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXisVKhs7o%3D&md5=d06fe1f8c951af29dd40340c9b187db6CAS | 15032844PubMed |
Poiatti VAD, Dalmas FR, Astarita LV (2009) Defense mechanisms of Solanum tuberosum L. in response to attack by plant-pathogenic bacteria. Biological Research 42, 205–215.
| Defense mechanisms of Solanum tuberosum L. in response to attack by plant-pathogenic bacteria.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXotVakurw%3D&md5=777efa929b582fe3c4e6e42dec369e13CAS | 19746266PubMed |
Ritchie LE, Steiner JM, Suchodolski JS (2008) Assessment of microbial diversity along the feline intestinal tract using 16S rRNA gene analysis. FEMS Microbiology Ecology 66, 590–598.
| Assessment of microbial diversity along the feline intestinal tract using 16S rRNA gene analysis.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXhsVyqu7bO&md5=706279df19738743a5425108f3c020eaCAS | 19049654PubMed |
Salkowski E (1885) Ueber das verhalten der skatolcarbonsäure im organismus. Zeitschrift für Physiologische Chemie 9, 23–33.
Sattler M (2006) Development of shoot and root system of juvenile Araucaria angustifolia (Bert.) O. Ktze. growing on different sites. PhD Thesis, Rottenburg University of Applied Forest Sciences.
Schrey SD, Tarkka MT (2008) Friends and foes: Streptomyces as modulators of plant disease and symbiosis. Antonie Van Leeuwenhock 94, 11–19.
| Friends and foes: Streptomyces as modulators of plant disease and symbiosis.Crossref | GoogleScholarGoogle Scholar |
Shimizu M, Meguro A, Hasegawa S, Nishimura T, Kunoh H (2006) Disease resistance induced by nonantagonistic endophytic Streptomyces spp. on tissue-cultured seedlings of rhododendron. Journal of General Plant Pathology 72, 351–354.
| Disease resistance induced by nonantagonistic endophytic Streptomyces spp. on tissue-cultured seedlings of rhododendron.Crossref | GoogleScholarGoogle Scholar |
Shirling EB, Gottlieb D (1966) Methods for characterization of Streptomyces species. International Journal of Systematic Bacteriology 16, 313–340.
| Methods for characterization of Streptomyces species.Crossref | GoogleScholarGoogle Scholar |
Suzuki T, Shimizu M, Meguro A, Hasegawa S, Nishimura T, Kunoh H (2005) Visualization of infection of an endophytic actinomycete Streptomyces galbus in leaves of tissue-cultured rhododendron. Actinomycetologica 19, 7–12.
| Visualization of infection of an endophytic actinomycete Streptomyces galbus in leaves of tissue-cultured rhododendron.Crossref | GoogleScholarGoogle Scholar |
Van der Ent S, Van Wees SCM, Pieterse CMJ (2009) Jasmonate signaling in plant interactions with resistance-inducing beneficial microbes. Phytochemistry 70, 1581–1588.
| Jasmonate signaling in plant interactions with resistance-inducing beneficial microbes.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXhtlGiu7fN&md5=fcc255d62621eb73d44f2e854aa8c715CAS | 19712950PubMed |
Whitham FH, Blaydes DF, Devlin RM (1971) ‘Experiments in plant physiology.’ (D van Nostrand Company: New York)
Zandavalli RB, Dillenburg LR, Souza PVD (2004) Growth responses of Araucaria angustifolia (Araucariaceae) to inoculation with the mycorrhizal fungus Glomus clarum. Applied Soil Ecology 25, 245–255.
| Growth responses of Araucaria angustifolia (Araucariaceae) to inoculation with the mycorrhizal fungus Glomus clarum.Crossref | GoogleScholarGoogle Scholar |
Zhang H, Xie X, Kim M-S, Kornyeyev DA, Holaday S, Paré PW (2008) Soil bacteria augment Arabidopsis photosynthesis by decreasing glucose sensing and abscisic acid levels in planta. The Plant Journal 56, 264–273.
| Soil bacteria augment Arabidopsis photosynthesis by decreasing glucose sensing and abscisic acid levels in planta.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXhtlChu7rI&md5=71c645ba1fe4f512e0bdb1bf15613448CAS | 18573192PubMed |
