Late-acting self-incompatibility in Acca sellowiana (Myrtaceae)1
Taciane Finatto A B D , Karine L. Dos Santos A B , Neusa Steiner A B , Leon Bizzocchi A , Daniel F. Holderbaum A , Jean P. H. J. Ducroquet C , Miguel P. Guerra A B and Rubens O. Nodari A BA Laboratório de Fisiologia do Desenvolvimento e Genética Vegetal, Departamento de Fitotecnia, Centro de Ciências Agrárias, Universidade Federal de Santa Catarina, Rodovia Admar Gonzaga 1346, Itacorubi, Florianópolis-SC, ZIP 88034-900, Brazil.
B Programa de Pós-Graduação em Recursos Genéticos Vegetais, Centro de Ciências Agrárias, Universidade Federal de Santa Catarina, Florianópolis, Santa Catarina, Brazil.
C Estação Experimental de São Joaquim, Empresa de Pesquisa Agropecuária e Extensão Rural do Estado de Santa Catarina, Rua João Araújo Lima, 102, Caixa Postal 81, Jardim Caiçara, São Joaquim-SC 88600-000, Brazil.
D Corresponding author. Email: tfinatto@gmail.com
Australian Journal of Botany 59(1) 53-60 https://doi.org/10.1071/BT10152
Submitted: 14 June 2010 Accepted: 21 November 2010 Published: 10 February 2011
Abstract
Acca sellowiana (Berg) Burret is a predominantly allogamous species with hermaphrodite flowers that has barriers to self-fertilisation such as dichogamy by protogyny and self-incompatibility. This study aimed to identify when self-incompatibility occurs in A. sellowiana flowers submitted to self-pollinations. Pollinations were made using nine known self-incompatible accessions in several treatments including manual cross-pollination, manual self-pollination, no pollination and natural pollination. Flowers were pollinated and pistils collected at times ranging from 1 to 30 days after pollination (DAP). In both cross- and self-pollinations the fertilisation occurred from 18 DAP and the zygote was visualised at 24 and 26 DAP, respectively. The abscission of the self-pollinated flowers increased from 26 DAP onwards, when significant differences (P < 0.05) were found for the percentage of abscission among self-pollinated and cross-pollinated flowers. At 30 DAP, 72% of self-pollinated flowers were abscised. The size of ovules and ovaries of self-pollinated flowers showed no significant difference from those of non-pollinated flowers throughout 30 DAP, while those from cross-pollinated flowers were significantly larger (P < 0.05) than the other treatments from 22 and 24 DAP, respectively. After 40 DAP, there was no fruit development in self-pollination and non-pollination treatments. Our study brings greater clarity to the mechanism of self-incompatibility in A. sellowiana, indicating late-acting self-incompatibility occurring through the rejection/abscission of self-pollinated flowers precisely after syngamy and zygote formation.
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