Nutrient responses differ between node and organogenic cultures of Corymbia torelliana × C. citriodora (Myrtaceae)
Cao Dinh Hung A and Stephen J. Trueman A B CA School of Science and Education, University of the Sunshine Coast, Maroochydore DC, Qld 4558, Australia.
B Agri-Science Queensland, University of the Sunshine Coast, Maroochydore DC, Qld 4558, Australia.
C Corresponding author. Email: strueman@usc.edu.au
Australian Journal of Botany 58(5) 410-419 https://doi.org/10.1071/BT10029
Submitted: 27 January 2010 Accepted: 26 May 2010 Published: 21 July 2010
Abstract
Hybrids between Corymbia torelliana (F.Muell.) K.D.Hill & L.A.S.Johnson and C. citriodora subsp. variegata (F.Muell.) A.R.Bean & M.W.McDonald are used extensively for plantation forestry in subtropical eastern Australia; however, plantation establishment has been hampered by inadequate seed supply and variable amenability to propagation as rooted cuttings. The present study investigated node-culture and organogenic-culture methods for in vitro propagation of two families of juvenile Corymbia hybrids. The effects of nutrient and auxin concentrations on root formation, shoot elongation and shoot proliferation, and subsequent conversion to plantlets in an in vitro soil-less system, were assessed. The response to the nutrient concentration differed between organogenic and node cultures. Half-strength, compared with full-strength, medium with auxin increased shoot proliferation by 58% for one family in the node culture, in which many shoots form roots and elongate rapidly in the absence of cytokinin. However, full-strength medium increased shoot proliferation by 213% over that in the half-strength medium for the other family in the organogenic culture, in which shoots do not form roots in the presence of cytokinin, and nutrient uptake occurs via callus and stem tissue rather than roots. The auxin, naphthalene acetic acid, sometimes stimulated root formation and shoot elongation in the node culture, although it had no effect on the final number of shoots. Most shoots (57–100%) subsequently formed roots in the in vitro soil-less medium. The high rates of proliferation and plantlet conversion in the present study provide the means, not only for simultaneous laboratory storage and field-testing of Corymbia clones before selection of desired genotypes, but also for en masse plantlet production of selected clones or families for plantation establishment.
Acknowledgements
We thank David Lee and Geoff Dickinson (Agri-Science Queensland) for providing Corymbia seeds and critical review of the manuscript, respectively. The project was funded by the Queensland National and International Research Alliances Program and supported by a postgraduate scholarship to CDH from the Vietnam Ministry of Education and Training.
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