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RESEARCH ARTICLE
Contents Vol 57(7)

Morphoanatomy and development of leaf secretory structures in Passiflora amethystina Mikan (Passifloraceae)

Diego Ismael Rocha A , Luzimar Campos da Silva A C , Vânia Maria Moreira Valente B , Dayana Maria Teodoro Francino A and Renata Maria Strozi Alves Meira A
+ Author Affiliations
- Author Affiliations

A Departamento de Biologia Vegetal, Universidade Federal de Viçosa (UFV), Av. P.H. Rolfs, s/n, Campus Universitário, CEP 36.570-000, Viçosa, MG, Brasil.

B Departamento de Química, Universidade Federal de Viçosa (UFV), CEP 36.570-000, Viçosa, MG, Brasil.

C Corresponding author. Email: luzimar@ufv.br

Australian Journal of Botany 57(7) 619-626 https://doi.org/10.1071/BT09158
Submitted: 10 September 2009  Accepted: 26 October 2009   Published: 21 December 2009

Abstract

Extrafloral nectaries (EFNs) are commonly found in Passiflora L. Reports have been made on the occurrence of resin-producing structures morphologically similar to EFNs in the genus. The objective of this study was to characterise the morphoanatomy and development of leaf secretory structures in Passiflora amethystina and to use chemical and histochemical tests to detect the presence of sugars in the exudates. Samples of leaf blade and petioles in different developmental stages were collected and subjected to usual techniques using light and scanning electron microscopy. Secretion samples were analysed by high performance liquid chromatography. The concentration of total sugars in the secretion amounted to 39.67% for blade EFNs and 52.82% for petiolar EFNs. EFNs consist of a secretory, uni- or bistratified palisade epidermis, arising from the protoderm by means of anticlinal and periclinal divisions, glandular parenchyma originated from the ground meristem, and xylem and phloem elements formed from the procambium. Exudate accumulated in a subcuticular space formed outside the epidermal cells from where it was then released. Histochemical tests showed a positive reaction for neutral polysaccharides. The results confirm that the leaf secretory structures are indeed extrafloral nectaries, and these findings constitute important information for studies on the taxonomy and ecology of this species.


Acknowledgements

We thank the Microscopy and Microanalysis Center of the Federal University of Viçosa; Gilmar Valente and Elton Valente for the help with the collections, Dr Armando Carlos Cervi for the species identification and the Research Support Foundation of the State of Minas Gerais (FAPEMIG) for the financial support to the first author.


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