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RESEARCH ARTICLE
Contents Vol 57(7)

Morphoanatomy and development of leaf secretory structures in Passiflora amethystina Mikan (Passifloraceae)

Diego Ismael Rocha A , Luzimar Campos da Silva A C , Vânia Maria Moreira Valente B , Dayana Maria Teodoro Francino A and Renata Maria Strozi Alves Meira A
+ Author Affiliations
- Author Affiliations

A Departamento de Biologia Vegetal, Universidade Federal de Viçosa (UFV), Av. P.H. Rolfs, s/n, Campus Universitário, CEP 36.570-000, Viçosa, MG, Brasil.

B Departamento de Química, Universidade Federal de Viçosa (UFV), CEP 36.570-000, Viçosa, MG, Brasil.

C Corresponding author. Email: luzimar@ufv.br

Australian Journal of Botany 57(7) 619-626 https://doi.org/10.1071/BT09158
Submitted: 10 September 2009  Accepted: 26 October 2009   Published: 21 December 2009

Abstract

Extrafloral nectaries (EFNs) are commonly found in Passiflora L. Reports have been made on the occurrence of resin-producing structures morphologically similar to EFNs in the genus. The objective of this study was to characterise the morphoanatomy and development of leaf secretory structures in Passiflora amethystina and to use chemical and histochemical tests to detect the presence of sugars in the exudates. Samples of leaf blade and petioles in different developmental stages were collected and subjected to usual techniques using light and scanning electron microscopy. Secretion samples were analysed by high performance liquid chromatography. The concentration of total sugars in the secretion amounted to 39.67% for blade EFNs and 52.82% for petiolar EFNs. EFNs consist of a secretory, uni- or bistratified palisade epidermis, arising from the protoderm by means of anticlinal and periclinal divisions, glandular parenchyma originated from the ground meristem, and xylem and phloem elements formed from the procambium. Exudate accumulated in a subcuticular space formed outside the epidermal cells from where it was then released. Histochemical tests showed a positive reaction for neutral polysaccharides. The results confirm that the leaf secretory structures are indeed extrafloral nectaries, and these findings constitute important information for studies on the taxonomy and ecology of this species.


Acknowledgements

We thank the Microscopy and Microanalysis Center of the Federal University of Viçosa; Gilmar Valente and Elton Valente for the help with the collections, Dr Armando Carlos Cervi for the species identification and the Research Support Foundation of the State of Minas Gerais (FAPEMIG) for the financial support to the first author.


References


APG (2003) An update of the Angiosperm Phylogeny Group classification for the orders and families of flowering plants: APG II. Botanical Journal of the Linnean Society 141, 399–436.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Beckmann RL, Stucky JM (1981) Extrafloral nectaries and plant guarding in Ipomea pandurata (L.) G.F.W. Mey. (Convolvulaceae). American Journal of Botany 68, 72–79.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Cervi AC (1997) Passifloraceae do Brasil. Estudo do gênero Passiflora L., subgênero Passiflora. Fontqueria 45, 1–92. open url image1

Dave YS, Patel ND (1975) A developmental study of extrafloral nectaries in slipper spurge (Pedilanthus tithymaloides, Euphorbiaceae). American Journal of Botany 62(8), 808–812.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Deginani NB (2001) Las especies argentinas del género Passiflora (Passifloraceae). Darwiniana 39, 43–129. open url image1

Díaz-Castelazo C, Rico-Gray V, Ortega F, Ángeles G (2005) Morphological and secretory characterization of extrafloral nectaries in plants of coastal Veracruz, Mexico. Annals of Botany 96, 1175–1189.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Durkee L (1982) The floral and extrafloral nectaries of Passiflora. II. The extrafloral nectary. American Journal of Botany 69, 1420–1428.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Durkee L, Baird CH, Cohen P (1984) Light and electron microscopy of the resin glands of Passiflora foetida (Passifloraceae). American Journal of Botany 71, 596–602.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Elias TS (1983) Extrafloral nectaries: their structure and distribution. In ‘The Biology of Nectaries’. (Eds BL Bentley, TS Elias) pp. 174–203. (Columbia University Press: New York)

Elias TS, Gelband H (1977) Morphology, anatomy and relationship of extrafloral nectarines and hydathodes in two species of Impatiens (Balsaminaceae). Botanical Gazette (Chicago, Ill.) 138, 206–212.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Elias TS, Rozich WR, Newcombe L (1975) The Foliar and Floral Nectaries of Turneara ulmifolia L. American Journal of Botany 62, 570–576.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Farinazzo NM, Salimena FRG (2007) Passifloraceae na Reserva Biológica da Represa do Grama, Descoberto, Minas Gerais, Brasil. Rodriguésia 58, 823–833. open url image1

Fahn A (1979) ‘Secretory tissues in plants’. 1st edn. (Academic Press: London) 312 pp.

Fahn A (2000) Structure and function of secretory cells. Advances in Botanical Research 31, 37–75.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Gallo MBC, Weinberg B (2007) Contribuição ao Conhecimento das espécies de Passiflora L. do município de Poços de Caldas-MG. Revista Brasileira de Biociências 5(2), 417–419. open url image1

Gonzalez AM (1996) Nectários extraflorales em Turnera series Canaligerae y Leiocarpae. Bonplandia 9, 129–143. open url image1

Gonzalez AM, Ocantos MN (2006) Nectarios Extraflorales en Piriqueta y Turnera (Turneraceae). Boletín de La Sociedad Argentina de Botánica 41(3–4), 269–284. open url image1

Heil M (2008) Indirect defence via tritrophic interactions. The New Phytologist 178, 41–61.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Heil M, Fiala B, Baumann B, Linsenmair KE (2000) Temporal, spatial and biotic variations in extrafloral nectar secretion by Macaranga tanarius. Functional Ecology 14, 749–757.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Heil M, Rattke J, Boland W (2005) Postsecretory hydrolysis of nectar sucrose and specialization in ant/plant mutualism. Science 308, 560–563.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Jáuregui D, García M, Pérez D (2001) Morfoanatomia de las glándulas secretoras de Passiflora guazumaefolia Juss. y Passiflora aff. P. tiliaefolia L. (Passifloraceae) presents en Venezuela. Phyton- Revista Internacional de Botanica Experimental 70, 229–235. open url image1

Jáuregui D, García M, Pérez D (2002) Morfoanatomía de las glándulas em cuatro espécies de Passiflora L. (Passifloraceae) de Venezuela. Caldasia 24, 33–40. open url image1

Jensen WA (1962) ‘Botanical histochemistry: Principles and practice.’ 1st edn. (WH Freeman: San Francisco) 408 pp.

Johansen DA (1940) ‘Plant microtechnique.’ 1st edn. (McGraw-Hill: New York) 523 pp.

Karnovsky MJ (1965) A formaldehyde-glutaraldehyde fixative of high osmolarity for use in electron microscopy. The Journal of Cell Biology 27, 137–138. open url image1

Koptur S (1992) Plants with extrafloral nectaries and ants in everglades habitats. The Florida Entomologist 75(1), 38–50.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Koptur S (1994) Floral and extrafloral nectars of Costa Rica Inga trees: a comparison of their constituents and composition. Biotropica 26, 276–284.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Lanza J (1988) Ant preferences for Passiflora nectar mimics that contain amino acids. Biotropica 20, 341–344.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Lapinjoki SP, Elo HA, Taipale HT (1991) Development and structure of resin glands on tissues of Betula pendula Roth, during growth. The New Phytologist 117, 219–223.
| Crossref | GoogleScholarGoogle Scholar | open url image1

McManus JF (1948) A Histological and histochemical uses of periodic acid. Stain Technology 23, 99–108.
| PubMed |
open url image1

Milward-de-Azevedo MA, Baumgratz JFA (2004) Passiflora L. subg. Decaloba (DC.) Rchb. (Passifloraceae) na região Sudeste. Rodriguesia 55(85), 17–54. open url image1

Milward-de-Azevedo MA, Valente MC (2004) Passifloraceae da mata de encosta do jardim botânico do Rio de janeiro e arredores, Rio de Janeiro, RJ. Arquivos do Museu Nacional. Museu Nacional (Brazil) 62, 367–374. open url image1

Nepi M (2007) Nectary structure and ultrastructure. In ‘Nectaries and Nectar’. 1st edn. (Eds S Nicolson, M Nepi, E Pacini) pp.129–166. (Springer: Dordrecht)

Nunes TS, Queiroz LP (2006) Flora da Bahia: Passifloraceae. Sitientibus 6, 194–226. open url image1

O’Brien TP , McCully ME (1981) ‘The study of plant structure principles and selected methods.’ 1st edn. (Termarcarphi Pty: Melbourne) 357 pp.

Paiva EAS, Machado SR (2005) Role of intermediary cells in Peltodon radicans (Lamiaceae) in the transfer of calcium and formation of calcium oxalate crystals. Brazilian Archives of Biology and Technology 48, 147–153.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Paiva EAS, Machado SR (2006) Ontogênese, anatomia e ultra-estrutura dos nectários extraflorais de Hymenaea stigonocarpa Mart. ex Hayne (Fabaceae : Caesalpinioideae). Acta Botânica Brasílica 20, 471–482. open url image1

Paiva EAS, Bueno RA, Delgado MN (2007) Distribution and structural aspects of extrafloral nectaries in Cedrela fissilis (Meliaceae). Flora 202, 455–461. open url image1

Pancini E , Nepi M (2007) Nectar production and presentation. In ‘Nectaries and Nectar’. (Eds S Nicolson, M Nepi, E Pacini) pp. 167–214. (Springer: Dordrecht)

Pearse AGE (1980) ‘Histochemistry theoretical and applied, Vol. 2.’ 4th edn. (Churchill Livingston: Edinburgh) 1518 pp.

Roth I (1974) Morfologia, anatomia y desarrollo de la hoja pinnada y de las glandulas laminales en Passiflora (Passifloraceae). Acta Botanica Venezuelica 9(1–4), 363–380. open url image1

Rudgers JA, Gardener MC (2004) Extrafloral nectar as a resource mediating multispecies interactions. Ecology 85(6), 1495–1502.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Schmid R (1988) Reproductive versus extra-reproductive nectarines – historical perspective and terminological recommendations. Botanical Review 54, 179–227.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Souza VC , Lorenzi H (2005) ‘Botânica Sistemática: Guia ilustrado para identificação das famílias de Angiospermas da flora brasileira, baseado em APG II.’ (Instituto Plantarum: Nova Odessa) 640 pp.

Thadeo M, Cassino MF, Vitarelli NC, Azevedo AA, Araújo JM, Valente VMM, Meira RMSA (2008) Anatomical and histochemical characterization of extrafloral nectaries of Prockia crucis (Salicaceae). American Journal of Botany 95(12), 1515–1522.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Ulmer T , MacDougal JM (2004) ‘Passiflora: Passionflowers of the world.’ (Timer Press: Cambridge) 430 pp.

Varassin IG, Penneys DS, Michelangeli FA (2008) Comparative anatomy and morphology of nectar-producing Melastomataceae. Annals of Botany 102, 899–909.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Vitta FA (2006) Flora De Grão-Mogol, Minas Gerais: Passifloraceae. Boletim de Botânica da Universidade de São Paulo 24, 9–12. open url image1