Epicuticular waxes and plant primary metabolites on the surfaces of juvenile Eucalyptus globulus and E. nitens (Myrtaceae) leaves

Martin J. Steinbauer; Noel W. Davies; Cyril Gaertner; Sylvie Derridj

doi:10.1071/BT09108

RESEARCH ARTICLE (Open Access)

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Epicuticular waxes and plant primary metabolites on the surfaces of juvenile Eucalyptus globulus and E. nitens (Myrtaceae) leaves

Martin J. Steinbauer ^A ^D , Noel W. Davies ^B , Cyril Gaertner ^C and Sylvie Derridj ^C

+ Author Affiliations

- Author Affiliations

^A Co-operative Research Centre for Sustainable Production Forestry/CSIRO Entomology, Canberra and Department of Zoology, La Trobe University, Melbourne, Victoria 3086, Australia.

^B Central Science Laboratory, University of Tasmania, Private Bag 74, Hobart, Tasmania 7001, Australia.

^C Institut National de la Recherche Agronomique, Unité 1272, Physiologie de l’Insecte-Signalisation et Communication, F 78 000 Versailles, France.

^D Corresponding author. Email: M.Steinbauer@latrobe.edu.au

Australian Journal of Botany 57(6) 474-485 https://doi.org/10.1071/BT09108
Submitted: 19 June 2009 Accepted: 22 September 2009 Published: 9 November 2009

Abstract

Our knowledge of the composition of the waxes on the surfaces of Eucalyptus leaves is growing but that of plant primary metabolites has been completely overlooked. The diffusion of primary metabolites above the cuticle exposes them to a variety of herbivorous taxa and has the potential to influence their responses to that plant. Juvenile leaves of two families of Eucalyptus globulus Labill. ssp. globulus and two families of E. nitens (Deane & Maiden) Maiden had 11 out of 16 of the epicuticular waxes that were detected in common. However, two phenylethyl esters (waxes) were only detected on leaves of one family of E. globulus and two benzyl esters (waxes) were not detected or were uncommon in samples from E. nitens. Wax compounds were generally found in samples from both leaf surfaces but a few were only detected in samples from particular sides. Species and families of eucalypt did not differ significantly in the concentrations of free sugars, polyols, malic acid or γ-aminobutyric acid (GABA) (all plant primary metabolites) collected from the surfaces of leaves. However, concentrations of all these metabolites were usually higher in collections from the upper surfaces of leaves. High wax abundance, especially on the lower surfaces of E. globulus leaves, is suspected to have hindered dissolution of all the primary metabolites quantified. Several free amino acids exhibited significant species-level differences in concentrations, namely the aromatic, amide and sulfur-containing amino acids as well as proline; family-level differences in amino acid concentrations were not significant. Australian and overseas evidence showing that differences in waxes and primary metabolites can be influential in plant susceptibility to herbivorous taxa is considered with respect to the threats posed by the autumn gum moth and Mycosphaerella leaf spot fungi.

Acknowledgements

Financial support for MJS to work at INRA in August–September 2003 was provided by the Australian Academy of Science under the Academy’s Scientific Visits to Europe program. The GC-MS used for the wax analyses was purchased with funds from the Australian Research Council’s Linkage Infrastructure, Equipment and Facilities scheme (reference number LE0345743). We thank Prof. Dr Caroline Müller (University of Bielefeld, Germany) for her advice on terminology and an anonymous reviewer for their constructive comments on an earlier draft of the manuscript.

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Appendix 1. Summary of statistically significant ANOVAs comparing quantities of sugars, polyols, malic acid and GABA collected from the leaves of *Eucalyptus globulus* and *E. nitens*
Non-significant analyses (except for total sugars) not shown and results of *post hoc* one-way ANOVAs given in text and of paired t-tests in Fig. 2

Appendix 2. Summary of statistically significant ANOVAs comparing quantities of amino acids collected from the leaves of *Eucalyptus globulus* and *E. nitens*
Non-significant analyses not shown and results of *post hoc* one-way ANOVAs given in text and of paired t-tests in Fig. 3