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RESEARCH ARTICLE
Contents Vol 57(5)

Essential oil variation in Eucalyptus crebra, E. melanophloia (Myrtaceae) and their hybrids

Joseph J. Brophy A C , Paul I. Forster B , Robert J. Goldsack A , D. Brynn Hibbert A and Acharaporn Punruckvong A
+ Author Affiliations
- Author Affiliations

A School of Chemistry, University of New South Wales, UNSW Sydney, NSW 2052, Australia.

B Queensland Herbarium, Environmental Protection Agency, Brisbane Botanic Gardens, Mt Coot-tha Road, Toowong, Qld 4066, Australia.

C Corresponding author. Email: J.Brophy@unsw.edu.au

Australian Journal of Botany 57(5) 425-431 https://doi.org/10.1071/BT08171
Submitted: 15 September 2008  Accepted: 6 July 2009   Published: 14 September 2009

Abstract

The leaf essential oils obtained from Eucalyptus melanophloia F.Muell., E. crebra F.Muell. and their hybrids from four widely separated sites were investigated. The major components that were observed, of the predominantly monoterpenoid oils, were α-pinene (1–58%), 1,8-cineole (0.2–60%) and p-cymene (0.2–21%). Oils tended to be unique to each locality investigated and although the parental species were discrete on their profiles, the oils of the hybrids appeared not to differ from those of their putative parents when commonly used methods of analysis (e.g. principal components analysis, PCA) were applied. Leaf oils are a complex mixture of components and elucidating differences among large datasets such as these can be difficult by traditional methods of analysis. Linear discriminant analysis (LDA) was applied to the oils data and it was found that with a forward stepwise LDA with 13 or 16 components of 70, it was possible to distinguish between the parents and their hybrids at a particular site or where the parents and hybrids originated.


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