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RESEARCH ARTICLE
Contents Vol 57(5)

Essential oil variation in Eucalyptus crebra, E. melanophloia (Myrtaceae) and their hybrids

Joseph J. Brophy A C , Paul I. Forster B , Robert J. Goldsack A , D. Brynn Hibbert A and Acharaporn Punruckvong A
+ Author Affiliations
- Author Affiliations

A School of Chemistry, University of New South Wales, UNSW Sydney, NSW 2052, Australia.

B Queensland Herbarium, Environmental Protection Agency, Brisbane Botanic Gardens, Mt Coot-tha Road, Toowong, Qld 4066, Australia.

C Corresponding author. Email: J.Brophy@unsw.edu.au

Australian Journal of Botany 57(5) 425-431 https://doi.org/10.1071/BT08171
Submitted: 15 September 2008  Accepted: 6 July 2009   Published: 14 September 2009

Abstract

The leaf essential oils obtained from Eucalyptus melanophloia F.Muell., E. crebra F.Muell. and their hybrids from four widely separated sites were investigated. The major components that were observed, of the predominantly monoterpenoid oils, were α-pinene (1–58%), 1,8-cineole (0.2–60%) and p-cymene (0.2–21%). Oils tended to be unique to each locality investigated and although the parental species were discrete on their profiles, the oils of the hybrids appeared not to differ from those of their putative parents when commonly used methods of analysis (e.g. principal components analysis, PCA) were applied. Leaf oils are a complex mixture of components and elucidating differences among large datasets such as these can be difficult by traditional methods of analysis. Linear discriminant analysis (LDA) was applied to the oils data and it was found that with a forward stepwise LDA with 13 or 16 components of 70, it was possible to distinguish between the parents and their hybrids at a particular site or where the parents and hybrids originated.


References


Adams RP (2001) ‘Identification of essential oil components by gas chromatography/quadrupole mass spectrometry.’ 4th edn. (Allured Publishing Corp.: Carol Stream, IL)

Aishima T, Nagasawa M, Fukushima D (1979) Differentiation of the aroma quality of soy sauce by statistical evaluation of gas chromatic profiles. Journal of Food Science 44, 1723–1727.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Brooker I , Kleinig D (1994) ‘Field guide to eucalypts. Vol. 3.’ (Inkata Press: Melbourne)

Brooker MIH, Lassak EV (1981) The volatile leaf oils of Eucalyptus ovata Labill. and E. brookerana A.M.Gray (Myrtaceae). Australian Journal of Botany 29, 605–615.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Brophy JJ , Southwell IA (2002) Eucalyptus chemistry. In ‘Eucalyptus – the genus Eucalyptus’. (Ed. JJW Coppen) pp. 102–160. (Taylor and Francis: London)

Brophy JJ , House APN , Boland DJ , Lassak EV (1991) Digests of the essential oils of 111 species from northern and eastern Australia. In ‘Eucalyptus leaf oils – use, chemistry, distillation and marketing’. (Eds DJ Boland, JJ Brophy, APN House) (Inkata Press: Melbourne)

Chippendale G (1988) Eucalyptus, Angophora (Myrtaceae). In ‘Flora of Australia, Vol. 19’. (Australian Government Publishing Service: Canberra)

Drake DW (1981a) Reproductive success of two Eucalyptus hybrid populations. I. Generalised seed output model and comparison of fruit parameters. Australian Journal of Botany 29, 25–35.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Drake DW (1981b) Reproductive success of two Eucalyptus hybrid populations. II. Comparison of predispersal seed parameters. Australian Journal of Botany 29, 37–48.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Dungey HS, Potts BM, Whitham TG, Li H-F (2000) Plant genetics affects arthropod community richness and composition: evidence from a synthetic eucalypt hybrid population. Evolution 54, 1938–1946.
|  CAS | PubMed |
open url image1

Dunlop PJ, Bignell CM, Jackson JF, Hibbert DB (1995) Chemometric analysis of gas chromatographic data of oils from Eucalyptus species. Chemometrics and Intelligent Laboratory Systems 30, 59–67.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Dunlop PJ, Bignell CM, Hibbert DB (1997) Use of gas chromatograms of the essential leaf oils of the genus Eucalyptus for taxonomic purposes. Australian Journal of Botany 45, 1–13.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Gaydou EM, Randriamcharisoa RP, Bianchini JP, Llinas JP (1988) Multidimensional data analysis of essential oils. Application to ylang-ylang (Cananga odorata Hook Fil. et Thomson, Forma genuina) grades classification. Journal of Agricultural and Food Chemistry 36, 574–579.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Grayling PM, Brooker MIH (1996) Evidence for the identity of the hybrid Eucalyptus ‘brachyphylla’ (Myrtaceae) from morphology and essential-oil composition. Australian Journal of Botany 44, 1–13.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Griffin AR, Burgess IP, Wolf L (1988) Patterns of natural and manipulated hybridisation in the genus Eucalyptus L’Herit. – A review. Australian Journal of Botany 36, 41–66.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Heller SR , Milne GWA (1978) ‘EPA/NIH mass spectral data base.’ (US Government Printing Office: Washington, DC)

Heller SR , Milne GWA (1980) ‘EPA/NIH mass spectral data base.’ (US Government Printing Office: Washington, DC)

Heller SR , Milne GWA (1983) ‘EPA/NIH mass spectral data base.’ (US Government Printing Office: Washington, DC)

Hibbert DB (1997) Chemometric analysis of data from essential oils. In ‘Modern methods of plant analysis. Vol. 19’. (Eds HF Linskens, JF Jackson) pp. 119–140. (New Series, Springer: New York)

Holman JE, Hughes JM, Fensham RJ (2003) A morphological cline in Eucalyptus: a genetic perspective. Molecular Ecology 12, 3013–3025.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Joulain D , König WA (1998) ‘The atlas of spectral data of sesquiterpene hydrocarbons.’ (E. B. Verlag: Hamburg)

Levin DA (2000) ‘The origin, expansion, and demise of plant species.’ (Oxford University Press: Oxford)

Li H, Madden JL, Davies NW (1994) Variation in leaf oils of Eucalyptus nitens and E. denticulata. Biochemical Systematics and Ecology 22, 631–640.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Shepherd M, Chaparro JX, Teasdale R (1999) Genetic mapping of monoterpene composition in an interspecific eucalypt hybrid. Theoretical and Applied Genetics 99, 1207–1215.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Stenhagen E , Abrahamsson S , McLafferty FW (1974) ‘Registry of mass spectral data.’ (Wiley: New York)

Swigar AA , Silverstein RM (1981) ‘Monoterpenes.’ (Aldrich: Milwaukee, WI)