Hyperhydricity reversal and clonal propagation of four-wing saltbush (Atriplex canescens, Chenopodiaceae) cultivated in vitro
Isaac Reyes-Vera A C , Carol Potenza B and Jerry Barrow BA New Mexico State University, Las Cruces, New Mexico 88003, USA.
B United States Department of Agriculture, Agricultural Research Service, Jornada Experimental Range, Las Cruces, New Mexico 88003, USA.
C Corresponding author. Email: ireyes@nmsu.edu
Australian Journal of Botany 56(4) 358-362 https://doi.org/10.1071/BT07116
Submitted: 21 June 2007 Accepted: 28 February 2008 Published: 16 June 2008
Abstract
In vitro propagated shoots of four-wing saltbush (Atriplex canescens, Pursh Nutt) showed severe symptoms of hyperhydricity. We show that the reversion of hyperhydric A. canescens shoots to normal shoots was significantly affected by the presence of inorganic nitrogen in the culture vessel. When the culture vessel was vented or when ammonium nitrate was deleted from Murashige and Skoog basal medium, rates of reversion were significantly higher. Although statistically significant differences were evident when comparing vented v. non-vented treatments for each medium, the modified culture medium with vented closures was consistently the best treatment, showing a total cumulative frequency of 39.7% reversion to normal morphology, compared with a total cumulative frequency of 7.1% observed in the control treatment. Resulting normal shoots also showed significant improvements in further manipulations, including rooting in vitro, transplantation to soil and survival in native sites.
Andarwulan N, Kalidas S
(1999) Influence of acetyl salicylic acid in combination with fish protein hydrolyzates on hyperhydricity reduction and phenolic synthesis in oregano (Origanum vulgare) tissue cultures. Journal of Food Biochemistry 23, 619–635.
| Crossref | GoogleScholarGoogle Scholar |
Bean T,
Smith S, Karpiscak M
(2004) Intensive revegetation in Arizona’s hot desert, the advantage of container stock. Native Plants Journal 5, 173–180.
| Crossref |
Brand M
(1993) Agar and ammonium nitrate influence hyperhydricity, tissue nitrate and total nitrogen content of serviceberry (Amelanchier arborea) shoots in vitro. Plant Cell, Tissue and Organ Culture 35, 203–209.
| Crossref | GoogleScholarGoogle Scholar |
Colomer J, Passera C
(1990) The nutritional value of Atriplex spp. as fodder for arid regions. Journal of Arid Environments 19, 289–295.
Daguin F, Letouse R
(1984) Relations entre hypolignification et éta vitreux chez Salix babylonica en culture in vitro. Rôle de la nutrition ammoniacale. Canadian Journal of Botany 63, 324–326.
Daguin F, Letouse R
(1986) Ammonium-induced vitrification in cultured tissue. Physiologia Plantarum 66, 94–98.
| Crossref | GoogleScholarGoogle Scholar |
Debergh P,
Aitken-Christie J,
Cohen D,
Grout B,
Von Arnold S,
Zimmerman R, Ziv M
(1992) Reconsideration of the term ‘Vitrification’ as used in micropropagation. Plant Cell, Tissue and Organ Culture 30, 135–140.
| Crossref | GoogleScholarGoogle Scholar |
Eguchi Y,
Milazzo MC,
Ueno K, Kalidas S
(1999) Partial improvement of vitrification and acclimation of Oregano (Origanum vulgare L.) tissue cultures by fish protein hydrolizates. Journal of Herbs, Spice & Medicinal Plants 6, 29–38.
| Crossref | GoogleScholarGoogle Scholar |
Fal MA,
Majada JP,
González A, Sánchez Tamés R
(1999) Differences between Dianthus caryophyllus L. cultivar in in vitro growth and morphogenesis are related to their ethylene production. Plant Growth Regulation 27, 131–136.
| Crossref | GoogleScholarGoogle Scholar |
Ferreira C, Handro W
(1988) Micropropagation of Stevia rebaudiana through leaf explants from adult plants. Planta Medica 54, 157–160.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Franck T,
Gaspar T,
Kevers C,
Penel C,
Dommes J, Hausman JF
(2001) Are hyperhydric shoots of Prunus avium L. energy deficient? Plant Science 160, 1145–1151.
| Crossref | GoogleScholarGoogle Scholar |
Hazarika BN
(2006) Morpho-physiological disorders in in vitro culture of plants. Scientia Horticulturae 108, 105–120.
| Crossref | GoogleScholarGoogle Scholar |
Kenny L, Caligari PDS
(1996) Androgenesis of the salt tolerant shrub Atriplex glauca. Plant Cell Reports 15, 829–832.
| Crossref | GoogleScholarGoogle Scholar |
Kevers C, Gaspar T
(1985) Vitrification of carnation in vitro: Changes in ethylene production, ACC level and capacity to convert ACC to ethylene. Plant Cell, Tissue and Organ Culture 4, 215–223.
| Crossref | GoogleScholarGoogle Scholar |
Kevers C,
Coumans M,
Coumans-Gilles MF, Gaspar T
(1984) Physiological and biochemical events leading to vitrification of plants cultured in vitro. Physiologia Plantarum 61, 69–74.
| Crossref | GoogleScholarGoogle Scholar |
Khalil KJ,
Sawaya WN, Hyder SZ
(1986) Nutrient composition of Atriplex leaves grown in Saudi Arabia. Journal of Range Management 39, 104–107.
| Crossref | GoogleScholarGoogle Scholar |
Kidelman Dantas de Oliveira A,
Cañal MJ,
Centeno ML,
Feito I, Fernández B
(1997) Endogenous plant growth regulators in carnation tissues under different conditions of ventilation. Plant Growth Regulation 22, 169–174.
| Crossref | GoogleScholarGoogle Scholar |
Lai CH,
Lin HM,
Nalawade SM,
Fang W, Tsay HS
(2005) Hyperhydricity in shoots cultures of Scrophularia yoshimurae can be effectively reduced by ventilation of culture vessels. Journal of Plant Physiology 162, 355–361.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Marks T, Sally S
(2000) Interaction of explant type and indole-3- butyric acid during rooting in vitro in a range of difficult and easy–to-root woody plants. Plant Cell, Tissue and Organ Culture 62, 65–74.
| Crossref | GoogleScholarGoogle Scholar |
Murashige T, Skoog F
(1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15, 473–497.
| Crossref | GoogleScholarGoogle Scholar |
Pâques M
(1991) Vitrification and micropropagation: causes, remedies and prospects. Acta Horticulturae 289, 283–290.
Pasqualetto PL,
Zimmerman RH, Fordham I
(1986) Gelling agent and growth regulator effects on shoots vitrification of ‘Gala’ apple in vitro. Journal of the American Society for Horticultural Science 11, 976–980.
Phillips GC, Collins GB
(1979) In vitro tissue culture of selected legumes and plant regeneration of red clover. Crop Science 19, 59–64.
Saher S,
Piqueras A,
Hellin E, Olmos E
(2004) Hyperhydricity in micropropagated carnation shoots: the role of oxidative stress. Physiologia Plantarum 120, 152–161.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Salo L,
Artiola J, Goodrich-Mahoney J
(1999) Evaluation of Revegetation Techniques of a Saline Flue Gas Desulfurization Sludge Pond. Journal of Environmental Quality 8, 218–225.
Schloupf RM,
Barringer SA, Splittstoesser WE
(1995) A review of hyperhydricity (vitrification) in tissue culture. Plant Growth Regulation Society of America Quarterly 23, 149–158.
White PR
(1934) Potentially unlimited growth of excised tomato root tips in a liquid medium. Plant Physiology 9, 585–600.
| PubMed |
Wochok ZS, Sluis CJ
(1980) Gibberelic acid promotes Atriplex shoot multiplication and elongation. Plant Science Letters 17, 363–369.
| Crossref | GoogleScholarGoogle Scholar |
Wurtele ES,
Garton S,
Young D,
Balandrin M, McKell CM
(1987) Propagation of an elite high biomass-producing genotype of Atriplex canescens by axillary enhancement. Biomass 12, 281–291.
| Crossref | GoogleScholarGoogle Scholar |
Zimmerman T
(1996) Genetic transformation through the use of hyperhydric tobacco meristems. Molecular Breeding 20, 73–80.
Ziv M,
Schawartz A, Fleminger D
(1987) Malfunctioning stomata in vitreous leaves of carnation (Dianthus caryiophyllus) plants propagated in vitro; implications for hardening. Plant Science 52, 127–134.
| Crossref | GoogleScholarGoogle Scholar |
