Differential secretion of lactate and activity of plasma membrane H+-ATPase in the roots of soybean seedlings in response to low-oxygen stress
Hong Shen A B D , Weibin Jing A , Tiancheng Ai A , Ying Lu B and Jianxun Cheng CA Engineering and Technology Research Center of Hubei Waterlogging Lowland Department, Changjiang University, Jingzhou 434103, China.
B College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China.
C College of Life Science, South China Agricultural University, Guangzhou 510642, China.
D Corresponding author. Email: hshen@scau.edu.cn
Australian Journal of Botany 54(5) 471-477 https://doi.org/10.1071/BT05141
Submitted: 16 August 2005 Accepted: 21 December 2005 Published: 3 August 2006
Abstract
Plants display a series of tolerance mechanism following exposure to low-oxygen stress. Increased secretion of end production of carbohydrate catabolism and synthesis of stress-related proteins are important mechanisms enabling the plant to develop tolerance to anoxia stress. In this study, the secretion of lactate and the activity of plasma membrane H+-ATPase in a wild-type (WTS) and a cultivated soybean (CTS) were investigated in response to low-oxygen stress. Low oxygen (0.1% O2, anoxia) increased the secretion of lactate and reduced the activity of plasma membrane H+-ATPase and ATP content in a time-dependent manner. WTS showed greater root elongation and higher survivability than CTS. The higher lactate secretion coincided with the lower accumulation of lactate in WTS than in CTS. Anoxia decreased the cellular pH in soybean roots. Hypoxia (5% O2) increased the secretion of lactate and the activity of plasma membrane H+-ATPase. In comparison to anoxia, hypoxia stress induced increases of 57.4 and 29.7% of endogenous abscisic acid (ABA) in the root apices of WTS and CTS, respectively. Exogenous application of ABA showed a stimulatory effect on the activity of plasma membrane H+-ATPase and the secretion of citrate from soybean roots. However, cycloheximide, an inhibitor of protein synthesis, abolished ABA effects. These results suggest that the modulation of the secretion of lactate and activity of plasma membrane H+-ATPase in soybean roots is associated with the mechanisms of tolerance to low-oxygen stress. ABA might be involved in the hypoxia signal transmitted in soybean roots.
Acknowledgments
This research was supported by the Foundation of Engineering and Technology Research Center of Hubei Waterlogging Lowland (HNKFJ2002C04), Changjiang University, National Natural Science Foundation of China (30471040, 30100110), International Foundation for Science (C/3042-1, 2), Guangdong Province Natural Science Foundation (No. 000642) and the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry.
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