Genetic variation, taxonomy and mountain-hopping of four bipolar Carex species (Cyperaceae) analysed by AFLP fingerprinting
K. Vollan A , O. M. Heide A , K. A. Lye A and M. Heun A BA Department of Ecology and Natural Resource Management, Norwegian University of Life Sciences, PO Box 5003, NO-1432 Ås, Norway.
B Corresponding author. Email: manfred.heun@umb.no
Australian Journal of Botany 54(3) 305-313 https://doi.org/10.1071/BT04214
Submitted: 9 December 2004 Accepted: 31 October 2005 Published:
Abstract
The bipolar sedges Carex canescens, C. echinata, C. lachenalii and C. magellanica (including C. paupercula) were analysed by amplified fragment length polymorphisms (AFLPs). The genetic variation of Carex populations from northern Europe was compared with those from the farthest locations in the Southern Hemisphere (i.e. Australia, New Zealand and Chile). This DNA fingerprinting of 152 plants yielded 223 AFLP bands, which were scored as present/absent and converted to Jaccard’s dichotomy coefficients. Unweighted pair-group methods using arithmetic averages (UPGMA), neighbour-joining (NJ), and principal coordinate analysis (PCA) were performed. We obtained AFLP-based separations of the four Carex species that were in full agreement with previous morphology-based taxonomy. A large number of species-specific bands occurred. Hemisphere-specific bands were not identified, but all analyses showed a clear distinction between populations collected from the Northern and Southern Hemispheres. The results are discussed in relation to the mountain-hopping hypothesis, which is one possible mechanism underlying the bipolar distribution of those species.
Acknowledgments
We gratefully acknowledge Drs Elizabeth Edgar and Peter N. Johnson of Landcare Research New Zealand for the collection of C. lachenalii in New Zealand, Dr Karen L. Wilson, Royal Botanic Gardens, Sydney, and Ms Genevieve Wright, New South Wales National Parks and Wildlife Service, for help with the localisation of C. canescens and C. echinata in Kosciuszko National Park, Australia. Thanks are also due to CSIRO Plant Industry in Canberra, for logistic assistance and support. Mr Per Salvesen, Arboretum and Botanical Garden, University of Bergen, and Mr Arve Elvebakk, University of Tromsø, kindly collected seeds of C. canescens and C. magellanica in Chile. The laboratory expenses were covered by a grant from the Research Council of Norway; project number NFR 148083/730. Thanks also go to Professor Jon Swenson for improving the English.
Backeljau T,
De Bruyn L,
De Wolf H,
Jordaens K,
Van Dongen S, Winnepenninckx B
(1996) Multiple UPGMA and neighbor-joining trees and the performance of some computer packages. Molecular Biology and Evolution 13, 309–313.
Ball PW
(1990) Some aspects of the phytogeography of Carex. Canadian Journal of Botany 68, 1462–1472.
Choler P,
Erschbaumer B,
Tribsch A,
Gielly L, Taberlet P
(2004) Genetic introgression as a potential to widen a species’ niche: insights from Carex curvula. Proceedings of the National Academy of Sciences, USA 101, 171–176.
| Crossref | GoogleScholarGoogle Scholar |
Després L,
Gielly L,
Redoutet B, Taberlet P
(2003) Using AFLP to resolve phylogenetic relationships in a morphologically diversified plant species complex when nuclear and chloroplast sequences fail to reveal variability. Molecular Phylogenetics and Evolution 27, 185–196.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Drossou A,
Katsiotis A,
Leggett JM,
Loukas M, Tsakas S
(2004) Genome and species relationships in genus Avena based on RAPD and AFLP molecular markers. Theoretical and Applied Genetics 109, 48–54.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Du Rietz GE
(1940) Problems of bipolar plant distribution. Acta Phytogeographica Suecica 13, 215–282.
El-Rabey HA,
Badr A,
Schäfer-Pregl R,
Martin W, Salamini F
(2002) Speciation and species separation in Hordeum L. (Poaceae) resolved by discontinuous molecular markers. Plant Biology 4, 567–575.
| Crossref | GoogleScholarGoogle Scholar |
Ford BA,
Starr JR, McQueens DAR
(1998) Relationships among species of Carex sect. Phyllostachys (Cyperaceae) based on allozyme divergence. Plant Systematics and Evolution 212, 31–51.
| Crossref | GoogleScholarGoogle Scholar |
Heide OM
(2002) Climatic flowering requirements of bipolar sedges Carex spp. and the feasibility of their trans-equatorial migration by mountain-hopping. Oikos 99, 352–362.
| Crossref | GoogleScholarGoogle Scholar |
Heide OM
(2004) Environmental control of flowering and sex expression in Carex flava. Physiologia Plantarum 121, 691–698.
| Crossref | GoogleScholarGoogle Scholar |
Hendrichs M,
Oberwinkler F,
Begerow D, Bauer R
(2004) Carex subgenus Carex (Cyperaceae)—A phylogenetic approach using ITS sequences. Plant Systematics and Evolution 246, 89–107.
| Crossref | GoogleScholarGoogle Scholar |
Jaccard P
(1908) Nouvelles rescherches sur la distribution florale. Bulletin de la société vaudoise des scences naturelle 44, 223–270.
Kardolus JP,
van Eck HJ, van den Berg RG
(1998) The potential of AFLPs in biosystematics: a first application in Solanum taxonomy (Solanaceae). Plant Systematics and Evolution 210, 87–103.
| Crossref | GoogleScholarGoogle Scholar |
Kropf M,
Kadereit JW, Comes HP
(2003) Differential cycles of range contraction and expansion in European high mountain plants during the late Quaternary: insights from Pritzelago alpina (L.) O.Kuntze (Brassicaceae). Molecular Ecology 12, 931–949.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Moore DM
(1967) Chromosome numbers of Falkland Islands angiosperms. British Antarctic Survey Bulletin 14, 69–82.
Moore DM, Chater AO
(1971) Studies on bipolar species. I. Carex. Botaniska Notiser 124, 317–334.
Muasya AM,
Simpsom DA,
Chase MW, Culham A
(1998) An assessment of suprageneric phylogeny in Cyperaceae using rbcL DNA sequences. Plant Systematics and Evolution 211, 257–271.
| Crossref | GoogleScholarGoogle Scholar |
Nelmes E
(1951) The genus Carex in Malaysia. Reinwardtia 1, 221–450.
Petrie D
(1881) Descriptions of new species of Carex. Transaction of the New Zealand Institute 13, 332–333.
Roalson EH, Friar EA
(2004) Phylogenetic relationships and biogeographic patterns in North American members of Carex section Acrocystis (Cyperaceae) using nrDNA ITS and ETS sequence data. Plant Systematics and Evolution 243, 175–187.
| Crossref | GoogleScholarGoogle Scholar |
Roalson EH,
Columbus JT, Friar EA
(2001) Phylogenetic relationships in Cariceae (Cyperaceae) based on ITS (nrDNA) and trn T-L-F (cpDNA) region sequences: assessment of subgeneric and sectional relationships in Carex with special emphasis on section Acrocystis. Systematic Botany 26, 318–341.
Rokas A,
Williams BL,
King N, Caroll SB
(2003) Genome-scale approaches to resolving incongruence in molecular phylogenies. Nature 425, 798–804.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Saitou N, Nei M
(1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4, 406–425.
| PubMed |
Sasanuma T,
Chabane K,
Endo TR, Valkoun J
(2004) Characterization of genetic variation in and phylogenetic ralationships among diploid Aegilops species by AFLP: incongruity of chloroplast and nuclear data. Theoretical and Applied Genetics 108, 612–618.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Schoenswetter P,
Tribsch A,
Schneeweiss GM, Niklfeld H
(2003) Disjunctions in relict alpine plants: phylogeography of Androsace brevis and A. wulfeniana (Primulaceae). Botanical Journal of the Linnean Society 141, 437–446.
| Crossref | GoogleScholarGoogle Scholar |
Semerikov VL,
Zhang H,
Sun M, Lascoux M
(2003) Conflicting phylogenies of Larix (Pinaceae) based on cytoplasmic and nuclear DNA. Molecular Phylogenetics and Evolution 27, 173–184.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Skottsberg C
(1960) Remarks on the plant geography of the southern cold temperate zone. Proceedings of the Royal Society of London. Series B. Biological Sciences 152, 447–457.
Starr JR,
Bayer RJ, Ford BA
(1999) The phylogenetic position of Carex section Phyllostachys and its implications for phylogeny and subgeneric circumscription in Carex (Cyperaceae) American Journal of Botany 86, 563–577.
| Crossref |
PubMed |
Steffen H
(1939) Ueber die floristische Beziehungen der beiden Polargebiete zueinander. Beihefte Botanisches Centralblatt 59B, 531–560.
Sudupak MA, Kence A
(2004) Genetic relationships among perennial and annual Cicer species growing in Turkey assessed by AFLP fingerprinting. Genetic Resources and Crop Evolution 51, 241–249.
| Crossref | GoogleScholarGoogle Scholar |
Takezaki N
(1998) Tie trees generated by distance methods of phylogenetic reconstruction. Molecular Biology and Evolution 15, 727–737.
| PubMed |
Toivonen H
(1979) The status of the New Zealand Carex lachenalii (Cyperaceae). Annales Botanici Fennici 16, 151–156.
Toivonen H
(1981) Notes on the nomenclature and taxonomy of Carex canescens (Cyperaceae). Annales Botanici Fennici 18, 91–97.
Ude G,
Pillay M,
Nwakanma D, Tenkouano A
(2002) Analysis of genetic diversity and sectional relationships in Musa using AFLP markers. Theoretical and Applied Genetics 104, 1239–1245.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Vos P,
Hogers R,
Bleeker M,
Reijans M,
van de Lee T,
Hornes M,
Frijters A,
Pot J,
Peleman J,
Kuiper M, Zabeau M
(1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acid Research 23, 4407–4414.
Waterway MJ, Olmstead RG
(1998) Phylogenetic relationships in Carex section Hymenochlaena inferred from non-coding nuclear and chloroplast DNA sequence data. American Journal of Botany abstract 85, 165–
.
Wilson JB
(1991) A comparison of biographic models: migration, vicariance and panbiogeography. Global Ecological and Biographical Letters 1, 84–87.
Winfield MO,
Wilson M,
Labra M, Parker JS
(2003) A brief evolutionary excursion comes to an end: the genetic relationship of British species of Gentianella sect. Gentianella (Gentianaceae). Plant Systematics and Evolution 237, 137–151.
| Crossref | GoogleScholarGoogle Scholar |
Yen AC, Olmstead RG
(2000) Molecular systematics of Cyperaceae tribe Cariceae based on two chloroplast DNA regions: ndhF and trnL intron-intergenic spacer. Systematic Botany 25, 479–494.
| Crossref |